##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545245_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	168748
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14828620191054	31.0	31.0	33.0	30.0	34.0
2	31.408330765401665	31.0	31.0	34.0	30.0	34.0
3	31.513700903121816	31.0	31.0	34.0	30.0	34.0
4	35.27255434138479	37.0	35.0	37.0	33.0	37.0
5	35.1244518453552	37.0	35.0	37.0	32.0	37.0
6	35.11460876573352	37.0	35.0	37.0	32.0	37.0
7	35.79392348353758	37.0	35.0	37.0	35.0	37.0
8	35.86877474103397	37.0	35.0	37.0	35.0	37.0
9	37.47646786924882	39.0	37.0	39.0	35.0	39.0
10	36.68765852039728	39.0	35.0	39.0	32.0	39.0
11	36.336715101808615	38.0	35.0	39.0	32.0	39.0
12	35.56519188375566	37.0	35.0	39.0	30.0	39.0
13	35.22887974968592	37.0	35.0	39.0	30.0	39.0
14	35.986583544693865	38.0	35.0	40.0	29.0	41.0
15	36.35601607130159	38.0	35.0	40.0	30.0	41.0
16	36.254586721027806	38.0	35.0	40.0	31.0	41.0
17	36.304211012871264	38.0	35.0	40.0	31.0	41.0
18	36.38023561760732	38.0	35.0	40.0	31.0	41.0
19	36.29848057458459	37.0	35.0	40.0	31.0	41.0
20	36.18330291321971	37.0	34.0	40.0	30.0	41.0
21	36.09064403726266	37.0	34.0	40.0	30.0	41.0
22	35.97894493564368	37.0	34.0	40.0	30.0	41.0
23	35.913527864033945	37.0	34.0	40.0	30.0	41.0
24	35.70934174034655	37.0	34.0	40.0	30.0	41.0
25	35.65917225685638	37.0	34.0	40.0	30.0	41.0
26	35.521078768340956	36.0	34.0	40.0	29.0	41.0
27	35.49318510441605	37.0	34.0	40.0	29.0	41.0
28	35.490204328347595	36.0	34.0	40.0	29.0	41.0
29	35.46384549742812	36.0	34.0	40.0	29.0	41.0
30	35.373888875720006	36.0	34.0	40.0	29.0	41.0
31	35.27125654822576	36.0	34.0	40.0	29.0	41.0
32	35.142644653566265	36.0	34.0	40.0	28.0	41.0
33	35.0056237703558	36.0	34.0	40.0	27.0	41.0
34	34.78554412496741	36.0	34.0	40.0	26.0	41.0
35	34.63692606727191	36.0	33.0	40.0	25.0	41.0
36	34.42531467039609	36.0	33.0	40.0	24.0	41.0
37	34.251742242871025	35.0	33.0	40.0	24.0	41.0
38	34.30516509825301	35.0	33.0	40.0	24.0	41.0
39	34.28283594472231	35.0	33.0	40.0	24.0	41.0
40	34.11949178656932	35.0	33.0	40.0	23.0	41.0
41	34.08363358380544	35.0	33.0	40.0	23.0	41.0
42	33.985060563680754	35.0	33.0	40.0	23.0	41.0
43	33.8879631165999	35.0	33.0	40.0	23.0	41.0
44	33.825017185388866	35.0	33.0	39.0	23.0	41.0
45	33.74121767369095	35.0	33.0	39.0	23.0	41.0
46	33.67072202337213	35.0	33.0	39.0	23.0	41.0
47	33.689122241448786	35.0	33.0	39.0	23.0	41.0
48	33.6121850333041	35.0	32.0	39.0	23.0	41.0
49	33.58464100315263	35.0	32.0	39.0	24.0	41.0
50	33.39974992296205	35.0	32.0	39.0	23.0	41.0
51	33.210248417759026	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	4.0
16	19.0
17	52.0
18	113.0
19	229.0
20	380.0
21	600.0
22	887.0
23	1215.0
24	1635.0
25	2222.0
26	2860.0
27	3431.0
28	4109.0
29	4584.0
30	5545.0
31	6570.0
32	8202.0
33	10388.0
34	16990.0
35	22713.0
36	12930.0
37	15565.0
38	20339.0
39	27147.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.38824756441558	24.362955412804894	27.925071704553535	14.323725318225994
2	31.67681987342072	25.757934908858175	29.32064380022282	13.24460141749828
3	29.375162964894397	26.333941735605755	30.74525327707588	13.545642022423971
4	26.96861592433688	27.740773223978948	30.032948538649347	15.25766231303482
5	22.79434422926494	33.44632232678313	29.080048356128664	14.679285087823263
6	21.919667196055656	41.03633820845284	26.52475881195629	10.519235783535212
7	86.57998909616707	5.030578140185365	6.570151942541541	1.8192808211060278
8	87.65259440111883	3.9816768198734205	6.472965605518288	1.8927631734894634
9	83.30350581932822	5.676511721620405	8.00128001517055	3.0187024438808163
10	49.6877000023704	24.543698295683505	14.054685092564059	11.713916609382037
11	42.30864958399507	21.731220518169103	21.26543721999668	14.694692677839145
12	38.67305093986299	21.39047573897172	25.109038329343164	14.827434991822125
13	22.675231706449857	36.17524355844217	24.73036717472207	16.419157560385898
14	16.835755090430702	38.746533292246426	27.58432692535615	16.833384691966717
15	15.73589020314315	24.238509493445846	43.52940479294569	16.49619551046531
16	18.33858771659516	19.503045962026217	41.154265532035936	21.00410078934269
17	17.450873491833978	20.370019200227556	29.942873397018037	32.236233910920426
18	21.82662905634437	23.836726953800934	33.86529025529191	20.47135373456278
19	30.034133737881337	24.599402659587078	25.34252257804537	20.023941024486216
20	30.800365041363452	23.535686348875245	25.74489771730628	19.919050892455022
21	23.5546495365871	28.6195984545002	27.152321805295472	20.673430203617226
22	22.24500438523716	25.324151989949513	24.23554649536587	28.19529712944746
23	20.441131154147012	30.125394078744637	25.90667741247304	23.526797354635313
24	19.88408751511129	24.82696091212933	38.172304264346835	17.116647308412546
25	19.95342167018276	26.038234527224024	33.86588285490791	20.142460947685308
26	17.760803090999598	33.27743143622443	26.693649702514993	22.268115770260984
27	18.201104605684215	33.45817431910304	28.14374096285586	20.19698011235689
28	16.57027046246474	29.543461255837105	35.288714532912984	18.59755374878517
29	17.43368810297011	25.773935098490057	34.21492402872923	22.577452769810606
30	20.195794913124896	31.305259914191574	29.816649678811007	18.68229549387252
31	29.375755564510392	26.91765235736127	24.649181027330695	19.05741105079764
32	30.165098253016332	25.28800341337379	26.002678550264303	18.544219783345582
33	29.56420224239695	26.5887595704838	23.49479697537156	20.352241211747696
34	20.80557991798421	26.278237371702183	27.505511176428758	25.410671533884848
35	22.11344727048617	25.090075141631306	30.546139806101408	22.25033778178112
36	29.823760874202954	27.191433379951167	24.087396591367007	18.89740915447887
37	20.829876502240026	34.39922250930381	26.386090501813353	18.384810486642806
38	21.17654727759736	33.81847488562828	24.541920496835516	20.463057339938846
39	21.580700215706262	32.56216369971792	24.572143077251283	21.28499300732453
40	25.113186526655127	25.811861473913766	26.366534714485507	22.7084172849456
41	18.827482399791403	24.75940455590585	27.245952544622753	29.167160499679994
42	19.85801313200749	26.81454002417806	24.491549529475908	28.83589731433854
43	20.47550193187475	26.15734705003911	26.32564534098182	27.041505677104322
44	19.133856401261053	28.4767819470453	30.137246071064546	22.252115580629102
45	18.44170004977837	35.61049612439851	24.255102282693723	21.6927015431294
46	20.423353165667148	34.28248038495271	25.272003223741912	20.022163225638227
47	21.591959608410175	28.026406238888757	27.26669353118259	23.114940621518475
48	21.218029250717045	26.033493730296065	31.586744731789413	21.161732287197477
49	21.394031336667695	27.340175883566026	31.42200203854268	19.8437907412236
50	19.596084101737503	33.07476236755399	27.717661838955127	19.611491691753383
51	18.915187142958732	32.47445895655059	26.566833384691968	22.043520515798708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	507.0
1	464.0
2	421.0
3	894.0
4	1367.0
5	1011.0
6	655.0
7	632.5
8	610.0
9	639.0
10	668.0
11	733.0
12	798.0
13	771.5
14	745.0
15	736.5
16	728.0
17	695.0
18	662.0
19	681.5
20	701.0
21	693.0
22	685.0
23	698.0
24	711.0
25	861.5
26	1192.0
27	1372.0
28	1840.0
29	2308.0
30	2792.0
31	3276.0
32	3649.5
33	4023.0
34	4715.5
35	5408.0
36	5517.5
37	5627.0
38	6453.0
39	7279.0
40	9174.0
41	11069.0
42	13285.5
43	15502.0
44	15684.0
45	15866.0
46	15039.5
47	14213.0
48	13033.5
49	11854.0
50	10631.0
51	9408.0
52	8849.0
53	8290.0
54	7631.5
55	6973.0
56	6743.0
57	6513.0
58	6473.0
59	6433.0
60	6077.0
61	5721.0
62	5250.0
63	4779.0
64	4197.0
65	3615.0
66	3134.0
67	2653.0
68	2291.5
69	1930.0
70	1736.5
71	1543.0
72	1362.0
73	1181.0
74	996.5
75	613.5
76	415.0
77	296.5
78	178.0
79	162.0
80	146.0
81	99.5
82	53.0
83	40.5
84	28.0
85	18.5
86	9.0
87	5.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	168748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.34982340531443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.63579689582528	28.380780809254034
2	9.447239061581897	7.8128333372839975
3	5.4831821373805125	6.801858392395761
4	3.8537053756968627	6.374001469647047
5	2.714361465812517	5.6119183634769
6	1.9906272840620836	4.93872519970607
7	1.4847299253335626	4.297532415198995
8	1.1020823480516502	3.645672837604001
9	0.8899780730039984	3.3120392537985635
>10	4.358169597431819	26.622537748595537
>50	0.025796465884173867	0.7081565411145614
>100	0.012898232942086934	1.0548273164718989
>500	0.001433136993565215	0.4391163154526275
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	741	0.4391163154526275	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	443	0.2625216298859838	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	296	0.17540948633465284	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	227	0.1345201128309669	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	179	0.10607533126318533	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02133358617583616	0.0
2	0.0	0.0	0.0	0.06340815891151302	0.0
3	0.0	0.0	0.0	0.10903832934316258	0.0
4	0.0	0.0	0.0	0.150520302462844	0.0
5	0.0	0.0	0.0	0.2743736222058928	0.0
6	0.0	0.0	0.0	0.4444497119965866	0.0
7	0.0	0.0	0.0	0.5641548344276673	0.0
8	0.0	0.0	0.0	0.8456396520255055	0.0
9	0.0	0.0	0.0	0.9878635598644132	0.0
10	0.0	0.0	0.0	1.2130514139426838	0.0
11	0.0	0.0	0.0	1.3973498945172684	0.0
12	0.0	0.0	0.0	1.5555739919880531	0.0
13	0.0	0.0	0.0	1.6219451489795436	0.0
14	0.0	0.0	0.0	1.651575129779316	0.0
15	0.0	0.0	0.0	1.679427311731102	0.0
16	0.0	0.0	0.0	1.761206058738474	0.0
17	0.0	0.0	0.0	1.8495034015217957	0.0
18	0.0	0.0	0.0	1.98698651243274	0.0
19	0.0	0.0	0.0	2.0527650698082347	0.0
20	0.0	0.0	0.0	2.1220992248797024	0.0
21	0.0	0.0	0.0	2.2133595657430014	0.0
22	0.0	0.0	0.0	2.318842297390191	0.0
23	0.0	0.0	0.0	2.4314362244293264	0.0
24	0.0	0.0	0.0	2.5179557683646623	0.0
25	0.0	0.0	0.0	2.587882523052125	0.0
26	0.0	0.0	0.0	2.672624268139474	0.0
27	0.0	0.0	0.0	2.7561808139948325	0.0
28	0.0	0.0	0.0	2.8385521606182	0.0
29	0.0	0.0	0.0	2.941664493801408	0.0
30	0.0	0.0	0.0	3.0613696162324886	0.0
31	0.0	0.0	0.0	3.1550003555597694	0.0
32	0.0	0.0	0.0	3.2658164837509185	0.0
33	0.0	0.0	0.0	3.37366961386209	0.0
34	0.0	0.0	0.0	3.4886339393652075	0.0
35	0.0	0.0	0.0	3.632635646052101	0.0
36	0.0	0.0	0.0	3.747007371939223	0.0
37	0.0	0.0	0.0	3.8756014886102355	0.0
38	0.0	0.0	0.0	4.009529001825207	0.0
39	0.0	0.0	0.0	4.2187166662716	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCGG	20	7.016165E-4	45.0	27
TGGGTAT	40	6.7557266E-9	45.0	6
AGGGTAC	80	0.0	45.0	6
TGCTGTA	20	7.016165E-4	45.0	26
TGATTCA	40	6.7557266E-9	45.0	44
TAGGGCG	85	0.0	45.0	5
GCGATGT	35	1.203698E-7	45.0	9
CCCCATT	80	0.0	45.0	42
TATAGCG	20	7.016165E-4	45.0	1
CGGTAGT	20	7.016165E-4	45.0	12
CCTATGC	40	6.7557266E-9	45.0	35
CGTCCCC	80	0.0	45.0	39
CGATCAA	20	7.016165E-4	45.0	10
CTAGGGA	45	3.8016879E-10	45.0	4
AATACGG	20	7.016165E-4	45.0	2
GCTTTGA	40	6.7557266E-9	45.0	40
AGCACGG	20	7.016165E-4	45.0	2
GCCTTAT	30	2.1544656E-6	44.999996	25
CATGCAT	60	0.0	44.999996	25
CCAGGGA	25	3.8766215E-5	44.999996	4
>>END_MODULE