FastQCFastQC Report
Thu 26 May 2016
SRR1545244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545244_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2796268
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131600.4706272789303458No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118600.4241367422578952No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC76250.2726848785595658No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33870.12112572900737698No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT33320.11915882168661944No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG2300.045.0000041
GTACGAC253.892244E-545.0000042
TACGAAC551.8189894E-1245.034
GCGATAT1950.045.09
CCGCGAA207.035024E-445.029
ACTCCGA351.2127566E-745.010
TCGGTAA453.8562575E-1045.037
TCGCGCG207.035024E-445.01
CTTACGC351.2127566E-745.034
ACGGTAC207.035024E-445.017
AAGTCGA351.2127566E-745.016
CCGTATG302.1666237E-644.99999635
CGTAGCT302.1666237E-644.99999633
CGACGGT2100.042.85714328
AACGAGT800.042.187529
GCGACGG2400.042.1874962
CGTTTTT90200.041.782151
GTCGATG1250.041.3999981
TAATGCG603.6379788E-1241.2499961
CTAATCC50600.041.1758911