##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545244_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2796268
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.271085604098033	31.0	31.0	34.0	30.0	34.0
2	31.513761198855047	31.0	31.0	34.0	30.0	34.0
3	31.59699928619145	31.0	31.0	34.0	30.0	34.0
4	35.34464436169924	37.0	35.0	37.0	33.0	37.0
5	35.19635135115804	37.0	35.0	37.0	32.0	37.0
6	35.18638699867108	37.0	35.0	37.0	32.0	37.0
7	35.856862432356266	37.0	35.0	37.0	35.0	37.0
8	35.97487222254805	37.0	35.0	37.0	35.0	37.0
9	37.6593845797327	39.0	37.0	39.0	35.0	39.0
10	36.881683372266174	39.0	37.0	39.0	32.0	39.0
11	36.44460688317429	38.0	35.0	39.0	32.0	39.0
12	35.217304278416805	35.0	35.0	39.0	30.0	39.0
13	34.57938974375847	35.0	33.0	39.0	27.0	39.0
14	35.25968290593033	36.0	33.0	40.0	27.0	41.0
15	35.8423555968169	36.0	34.0	40.0	30.0	41.0
16	35.88767135338959	36.0	34.0	40.0	31.0	41.0
17	35.98491668180589	36.0	34.0	40.0	31.0	41.0
18	36.099245494351756	36.0	35.0	40.0	31.0	41.0
19	36.01857654559577	36.0	35.0	40.0	31.0	41.0
20	35.842040176406556	36.0	34.0	40.0	31.0	41.0
21	35.66536004417316	35.0	34.0	40.0	30.0	41.0
22	35.55978003539003	35.0	34.0	40.0	30.0	41.0
23	35.53740163675299	35.0	34.0	40.0	30.0	41.0
24	35.38001078580451	35.0	34.0	40.0	30.0	41.0
25	35.37922438049572	35.0	34.0	40.0	30.0	41.0
26	35.26222951448145	35.0	34.0	40.0	30.0	41.0
27	35.20582397681481	35.0	34.0	40.0	29.0	41.0
28	35.297404969766845	36.0	34.0	40.0	30.0	41.0
29	35.33911484879132	36.0	34.0	40.0	30.0	41.0
30	35.26121208696734	36.0	34.0	40.0	30.0	41.0
31	35.089397725826	35.0	34.0	40.0	29.0	41.0
32	34.909937101880075	35.0	34.0	40.0	29.0	41.0
33	34.713504571092614	35.0	34.0	40.0	28.0	41.0
34	34.59963780295737	35.0	34.0	40.0	27.0	41.0
35	34.43265953048849	35.0	33.0	40.0	27.0	41.0
36	34.15886674667807	35.0	33.0	39.0	24.0	41.0
37	33.991212930949395	35.0	33.0	39.0	24.0	41.0
38	34.11289153972366	35.0	33.0	39.0	25.0	41.0
39	34.168267848432265	35.0	33.0	39.0	25.0	41.0
40	34.01817458126331	35.0	33.0	39.0	24.0	41.0
41	34.07293900298541	35.0	33.0	39.0	24.0	41.0
42	34.00053070735709	35.0	33.0	39.0	25.0	41.0
43	33.855514922031794	35.0	33.0	39.0	24.0	41.0
44	33.82899922325042	35.0	33.0	39.0	24.0	41.0
45	33.75274186880514	35.0	33.0	39.0	24.0	41.0
46	33.71657759556666	35.0	33.0	39.0	24.0	41.0
47	33.74400701220341	35.0	33.0	39.0	24.0	41.0
48	33.77325206310697	35.0	33.0	39.0	24.0	41.0
49	33.82418173079262	35.0	33.0	39.0	24.0	41.0
50	33.57282957141447	35.0	33.0	39.0	24.0	40.0
51	33.38113228059685	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	17.0
15	73.0
16	243.0
17	630.0
18	1470.0
19	2909.0
20	5066.0
21	8191.0
22	12476.0
23	17730.0
24	25720.0
25	35918.0
26	46795.0
27	56060.0
28	63384.0
29	73599.0
30	88974.0
31	110954.0
32	142275.0
33	188619.0
34	330255.0
35	509347.0
36	178163.0
37	204324.0
38	280509.0
39	412277.0
40	289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.01949884631945	24.639912912496225	29.68134670925677	13.659241531927554
2	29.41542083949035	27.282578064763463	30.50294177811283	12.79905931763336
3	26.689323054871707	26.661500256770807	32.78398207897097	13.86519460938651
4	24.98283426338248	29.227241451820785	31.50960494487653	14.280319339920208
5	21.8928586244237	32.69160895879794	30.57360739385495	14.84192502292341
6	20.564945849253363	41.7779340177694	27.02276748866704	10.634352644310201
7	86.83255682216439	5.393438683273563	6.1543457207964325	1.6196587737656045
8	89.22367240908238	3.637205017544813	5.492642336142316	1.6464802372304803
9	85.88643863892874	4.548777155837709	7.163512224150188	2.401271981083358
10	58.184337123623344	21.67227890888856	11.85522989928004	8.288154068208055
11	51.54373615118436	18.6919136506229	18.201867632143987	11.562482566048748
12	46.476267653887255	21.274641772533965	21.640772629805156	10.60831794377363
13	20.10358091570622	48.849573789064564	19.911038569979702	11.13580672524951
14	11.817679850429215	50.69131428031935	25.537251794177095	11.953754075074349
15	10.252522290424238	23.646445905757247	54.58475367883193	11.51627812498659
16	12.246251074646636	17.244555958155654	51.391247190898724	19.11794577629898
17	12.411399765687694	17.322624297814087	29.70587940783931	40.56009652865891
18	17.64601962329791	22.155279822964037	40.48864414998848	19.71005640374957
19	31.6116695538482	22.898913838015527	26.052402702459137	19.43701390567714
20	34.20112092260113	21.81554128574228	25.157459871514458	18.82587792014213
21	19.33484201085161	32.756767234041945	27.933695911836775	19.97469484326967
22	19.582171665949044	26.106045629388884	22.127492786814425	32.18428991784765
23	15.125874916138224	34.05313796817758	24.192852759463683	26.628134356220507
24	15.2287262880382	23.362066869126995	46.45187800310986	14.957328839724948
25	13.291894768312623	25.611672414804303	42.94688491947124	18.149547897411836
26	13.469810475962962	39.08101798540054	27.06067515703073	20.38849638160577
27	13.367531295283571	42.512520259145404	26.922419453357115	17.197528992213908
28	11.526863662567393	31.559957772287923	41.37042658285973	15.54275198228496
29	11.807559218215136	25.539504797108147	40.173402549397984	22.479533435278736
30	15.89307605708752	37.30586624744123	30.747124381497056	16.0539333139742
31	31.30107700692494	29.79453328507854	23.83720015391944	15.067189554077077
32	33.921069082076535	24.550937177695413	26.52066969260457	15.007324047623474
33	31.619465659228656	27.519429468133954	22.344675116977342	18.516429755660045
34	18.32252845578464	26.680024947537216	26.24866429111945	28.748782305558695
35	18.232050719029793	24.455488529711744	34.61989337216605	22.692567379092417
36	34.04176566766848	25.69549842861986	24.16456505599606	16.098170847715597
37	18.491718247321074	38.696720056875805	28.281302078341565	14.53025961746156
38	16.799462712443873	38.707341356407895	25.09945398652776	19.393741944620473
39	18.648033736394364	37.475342134587954	26.14516920409632	17.73145492492136
40	26.134869762125806	24.179978457000544	26.002300208706746	23.682851572166904
41	14.739431270536302	23.908259151125716	27.032029834050242	34.320279744287745
42	17.321551439275492	23.87875554131435	25.277763075642252	33.52192994376791
43	17.925856892114776	23.44660812196828	28.395704560507074	30.231830425409868
44	16.048640545183794	29.654131864327738	32.972197228591824	21.325030361896644
45	13.652446761183121	44.84580877083312	22.087332115519686	19.41441235246407
46	19.351077936735678	38.582782480077014	24.822370387959953	17.243769195227355
47	17.99405493321813	29.701909831246503	26.11992126648805	26.184113969047317
48	18.484673142917632	25.745851256031255	35.40576225168689	20.363713349364225
49	19.942509087111823	25.196261588660313	36.39887163891301	18.462357685314856
50	18.255653606878884	39.18293954656707	25.129708597316135	17.43169824923791
51	14.88598374690838	37.70672195941162	25.00511395903397	22.402180334646037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13213.0
1	10668.5
2	8124.0
3	14540.5
4	20957.0
5	14919.0
6	8881.0
7	9282.0
8	9683.0
9	11038.5
10	12394.0
11	13132.5
12	13871.0
13	13876.0
14	13881.0
15	13160.0
16	12439.0
17	11726.5
18	11014.0
19	11084.5
20	11155.0
21	10771.5
22	10388.0
23	12617.5
24	14847.0
25	15663.5
26	16596.0
27	16712.0
28	22863.0
29	29014.0
30	39417.0
31	49820.0
32	59891.0
33	69962.0
34	81393.0
35	92824.0
36	97477.0
37	102130.0
38	119065.0
39	136000.0
40	195099.5
41	254199.0
42	312442.0
43	370685.0
44	367654.0
45	364623.0
46	329803.0
47	294983.0
48	257552.0
49	220121.0
50	189908.0
51	159695.0
52	136381.0
53	113067.0
54	95001.0
55	76935.0
56	68944.0
57	60953.0
58	56133.5
59	51314.0
60	48738.0
61	46162.0
62	40128.0
63	34094.0
64	29715.0
65	25336.0
66	21156.5
67	16977.0
68	14144.5
69	11312.0
70	9820.5
71	8329.0
72	7066.0
73	5803.0
74	4625.5
75	2868.5
76	2289.0
77	1668.5
78	1048.0
79	816.5
80	585.0
81	395.0
82	205.0
83	175.0
84	145.0
85	126.5
86	108.0
87	67.0
88	26.0
89	20.5
90	15.0
91	15.0
92	15.0
93	10.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2796268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.629807872859287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.51043383536107	12.114731889886652
2	9.29059310856221	2.904203706234757
3	3.419721239978938	1.6034875787882077
4	1.7694195275351463	1.1062274904743916
5	1.0513888606387878	0.821650294572434
6	0.6844510504054135	0.6418703049728005
7	0.48257174117237606	0.5279752519576791
8	0.35651781017959383	0.44578439010876547
9	0.286384323595713	0.4028518760039781
>10	2.6386057330288115	9.677657242445926
>50	1.0412344359494323	11.90329720632381
>100	1.404225202638834	49.065228918365015
>500	0.057581289785821915	6.097006081081646
>1k	0.006160961047042675	1.4789745224564963
>5k	2.369600402708721E-4	0.2824025426021282
>10k+	4.739200805417442E-4	0.9266507037252782
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13160	0.4706272789303458	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11860	0.4241367422578952	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7625	0.2726848785595658	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3387	0.12112572900737698	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3332	0.11915882168661944	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.059722458648455726	0.0
2	0.0	0.0	0.0	0.19576092134230338	0.0
3	0.0	0.0	0.0	0.28337770199422946	0.0
4	0.0	0.0	0.0	0.3854780729171882	0.0
5	0.0	0.0	0.0	0.6760081651687178	0.0
6	0.0	0.0	0.0	0.9265563958819398	0.0
7	0.0	0.0	0.0	1.0969978557134008	0.0
8	0.0	0.0	0.0	1.5087967247774534	0.0
9	0.0	0.0	0.0	1.6861760031584956	0.0
10	0.0	0.0	0.0	1.975061045650846	0.0
11	0.0	0.0	0.0	2.2788945837809536	0.0
12	3.576195128650044E-5	0.0	0.0	2.547109218429707	0.0
13	7.152390257300088E-5	0.0	0.0	2.6492811132552387	0.0
14	7.152390257300088E-5	0.0	0.0	2.685400684054604	0.0
15	7.152390257300088E-5	0.0	0.0	2.7467324305109524	0.0
16	7.152390257300088E-5	0.0	0.0	2.8950730044473563	0.0
17	7.152390257300088E-5	0.0	0.0	3.0770298125930706	0.0
18	7.152390257300088E-5	0.0	0.0	3.392629032696437	0.0
19	7.152390257300088E-5	0.0	0.0	3.5061017041285027	0.0
20	7.152390257300088E-5	0.0	0.0	3.6247598584971112	0.0
21	7.152390257300088E-5	0.0	0.0	3.779323011957366	0.0
22	7.152390257300088E-5	0.0	0.0	3.9444359410471383	0.0
23	7.152390257300088E-5	0.0	0.0	4.158650029253276	0.0
24	1.7880975643250218E-4	0.0	0.0	4.318398665650074	0.0
25	1.7880975643250218E-4	0.0	0.0	4.433623672695178	0.0
26	1.7880975643250218E-4	0.0	0.0	4.551352016330338	0.0
27	1.7880975643250218E-4	0.0	0.0	4.66189220775691	0.0
28	1.7880975643250218E-4	0.0	0.0	4.779870885051075	0.0
29	1.7880975643250218E-4	0.0	0.0	4.918341160432405	0.0
30	1.7880975643250218E-4	0.0	0.0	5.109739123717755	0.0
31	1.7880975643250218E-4	0.0	0.0	5.274565957197236	0.0
32	1.7880975643250218E-4	0.0	0.0	5.416719713561075	0.0
33	1.7880975643250218E-4	0.0	0.0	5.55819399285047	0.0
34	1.7880975643250218E-4	0.0	0.0	5.724987733650709	0.0
35	1.7880975643250218E-4	0.0	0.0	5.963341138975234	0.0
36	2.145717077190026E-4	0.0	0.0	6.135856791981312	0.0
37	2.145717077190026E-4	0.0	0.0	6.295748476183256	0.0
38	2.5033365900550304E-4	0.0	0.0	6.457964687218822	0.0
39	2.5033365900550304E-4	0.0	0.0	6.621325280695555	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	230	0.0	45.000004	1
GTACGAC	25	3.892244E-5	45.000004	2
TACGAAC	55	1.8189894E-12	45.0	34
GCGATAT	195	0.0	45.0	9
CCGCGAA	20	7.035024E-4	45.0	29
ACTCCGA	35	1.2127566E-7	45.0	10
TCGGTAA	45	3.8562575E-10	45.0	37
TCGCGCG	20	7.035024E-4	45.0	1
CTTACGC	35	1.2127566E-7	45.0	34
ACGGTAC	20	7.035024E-4	45.0	17
AAGTCGA	35	1.2127566E-7	45.0	16
CCGTATG	30	2.1666237E-6	44.999996	35
CGTAGCT	30	2.1666237E-6	44.999996	33
CGACGGT	210	0.0	42.857143	28
AACGAGT	80	0.0	42.1875	29
GCGACGG	240	0.0	42.187496	2
CGTTTTT	9020	0.0	41.78215	1
GTCGATG	125	0.0	41.399998	1
TAATGCG	60	3.6379788E-12	41.249996	1
CTAATCC	5060	0.0	41.17589	11
>>END_MODULE