FastQCFastQC Report
Thu 26 May 2016
SRR1545243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545243_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4071561
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183610.450957261846255No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC82040.20149520048944372No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71350.17523991412630194No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG46460.11410856917039927No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT46320.11376472070540021No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG42860.1052667514989951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGC207.0354046E-445.014
TATATCG207.0354046E-445.040
CAATTCG502.1827873E-1145.01
TACGATC253.8925595E-545.011
ACGTCCA207.0354046E-445.014
TACGTCC253.8925595E-545.031
GCGTGTT600.044.99999644
TCACGAC3100.042.0967725
CGGTCTA3050.042.04918331
ATATACG750.042.01
GTCGATA700.041.7857138
TTACGAG1400.041.7857131
ACGTAAG1300.041.538461
TACGGGA10150.040.7881774
TAACGGG9800.040.4081653
TATAGCG1400.040.178571
CTAACGG1350.040.02
TCATCGA8500.039.44117416
CGGGAAC4000.039.3756
ATCGGAT403.4611185E-739.37516