##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545243_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4071561
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203161883120504	31.0	31.0	33.0	30.0	34.0
2	31.46335987597877	31.0	31.0	34.0	30.0	34.0
3	31.549933796890187	31.0	31.0	34.0	30.0	34.0
4	35.315683346018886	37.0	35.0	37.0	33.0	37.0
5	35.168807737376405	37.0	35.0	37.0	32.0	37.0
6	35.16564654195283	37.0	35.0	37.0	32.0	37.0
7	35.82552809598088	37.0	35.0	37.0	35.0	37.0
8	35.916353948768055	37.0	35.0	37.0	35.0	37.0
9	37.55525460628	39.0	37.0	39.0	35.0	39.0
10	36.7541294358601	39.0	37.0	39.0	32.0	39.0
11	36.40355357564334	38.0	35.0	39.0	32.0	39.0
12	35.45131068894707	37.0	35.0	39.0	30.0	39.0
13	34.996447062932376	37.0	34.0	39.0	29.0	39.0
14	35.78259001891412	38.0	34.0	40.0	28.0	41.0
15	36.23114500802026	38.0	35.0	40.0	30.0	41.0
16	36.173374290597636	38.0	35.0	40.0	31.0	41.0
17	36.251649428806296	37.0	35.0	40.0	31.0	41.0
18	36.31526164043717	37.0	35.0	40.0	31.0	41.0
19	36.24423237181022	37.0	35.0	40.0	31.0	41.0
20	36.108032029975725	37.0	34.0	40.0	31.0	41.0
21	35.95560842635048	37.0	34.0	40.0	30.0	41.0
22	35.85314330302309	36.0	34.0	40.0	30.0	41.0
23	35.816478004382105	36.0	34.0	40.0	30.0	41.0
24	35.63604646964641	36.0	34.0	40.0	30.0	41.0
25	35.62172444426106	36.0	34.0	40.0	30.0	41.0
26	35.472416107728705	36.0	34.0	40.0	29.0	41.0
27	35.4334077274048	36.0	34.0	40.0	29.0	41.0
28	35.47007449968206	36.0	34.0	40.0	29.0	41.0
29	35.474377763221526	36.0	34.0	40.0	29.0	41.0
30	35.39270859505728	36.0	34.0	40.0	29.0	41.0
31	35.265189198933776	36.0	34.0	40.0	29.0	41.0
32	35.12621621044116	36.0	34.0	40.0	29.0	41.0
33	34.9522313432121	36.0	34.0	40.0	27.0	41.0
34	34.80067227287028	36.0	34.0	40.0	27.0	41.0
35	34.63102136993649	35.0	33.0	40.0	26.0	41.0
36	34.43733865217787	35.0	33.0	40.0	25.0	41.0
37	34.275402480768435	35.0	33.0	40.0	24.0	41.0
38	34.3315340234372	35.0	33.0	40.0	25.0	41.0
39	34.345803734734666	35.0	33.0	40.0	25.0	41.0
40	34.181202246509386	35.0	33.0	40.0	24.0	41.0
41	34.183990120742386	35.0	33.0	40.0	24.0	41.0
42	34.14649098957378	35.0	33.0	40.0	24.0	41.0
43	34.004994153348065	35.0	33.0	40.0	24.0	41.0
44	33.97580043624546	35.0	33.0	40.0	24.0	41.0
45	33.92020234990953	35.0	33.0	39.0	23.0	41.0
46	33.849574647168495	35.0	33.0	39.0	23.0	41.0
47	33.87484726373988	35.0	33.0	39.0	23.0	41.0
48	33.86474720629263	35.0	33.0	39.0	23.0	41.0
49	33.848631765556256	35.0	33.0	39.0	24.0	41.0
50	33.644760080961575	35.0	33.0	39.0	24.0	41.0
51	33.44182440100983	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	12.0
12	11.0
13	25.0
14	39.0
15	145.0
16	373.0
17	1003.0
18	2262.0
19	4591.0
20	7905.0
21	12601.0
22	18633.0
23	26811.0
24	37889.0
25	52828.0
26	68657.0
27	82003.0
28	93791.0
29	108581.0
30	129563.0
31	159092.0
32	199341.0
33	258712.0
34	429151.0
35	609624.0
36	287089.0
37	344756.0
38	464581.0
39	670987.0
40	500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.21343804010304	23.770342627802947	28.490424188658846	13.52579514343516
2	31.65911059664831	25.550249646265893	29.448533375774062	13.342106381311739
3	29.09434489622039	25.818795297430153	31.136436369245114	13.950423437104345
4	26.574647905311995	28.0193272310055	30.799391191732116	14.606633671950389
5	23.045190775724596	32.503111214593126	29.067500155345826	15.384197854336456
6	21.51685803061774	41.584394781264486	26.50465018208004	10.394097006037734
7	86.14175251212004	5.110717977699462	6.747436671094944	2.00009283908555
8	87.51532888737268	4.064092371451637	6.521847517450924	1.8987312237247582
9	83.29218204025433	5.51670477244477	7.917307391440286	3.273805795860605
10	52.94824270101811	22.783325609023173	13.779776356046245	10.488655333912472
11	47.120649795004915	20.937620730722198	19.28650952300604	12.655219951266849
12	42.21641282053738	20.943073184952894	23.795345323329308	13.045168671180415
13	21.29856337655263	41.04536319116919	23.42256446606105	14.233508966217133
14	14.755814784550692	43.251224775952025	27.181270279384247	14.81169016011304
15	13.837469216352154	23.963757389364915	48.50535212416073	13.693421270122197
16	15.803668421030656	18.689146496884117	46.771299754565874	18.735885327519348
17	15.642821021225028	18.97522841976333	30.142837108421073	35.23911345059057
18	20.27347742057653	23.028563246381424	37.07455199615086	19.623407336891184
19	30.97946954497305	23.1003538937523	26.23067663728973	19.68949992398493
20	32.533296197699116	22.41602176659026	25.99366680248681	19.05701523322382
21	21.96803633790578	29.79405196188882	28.297844487654732	19.940067212550666
22	21.261796151402372	25.78497534483703	24.6356618505777	28.317566653182897
23	18.92635281652418	30.937814759498877	26.121529310257174	24.014303113719777
24	18.351438183045765	23.13842283094862	41.44933601633378	17.06080296967183
25	17.207478900598566	25.071538901173284	38.180835311076024	19.540146887152126
26	15.856645645245152	36.35274038630393	27.54189363735432	20.2487203310966
27	16.922232038277212	37.28601880212528	28.597238258250336	17.194510901347172
28	15.243883120012203	29.724938420424007	38.2558433976551	16.775335061908688
29	15.578398555246991	26.56229392117667	36.998610606595356	20.86069691698098
30	19.08422838316803	33.47996014305078	29.20597284432187	18.229838629459316
31	29.814609187974835	27.566970014694608	24.84474627790177	17.773674519428788
32	31.697940912588564	25.9701868644483	26.76496803068897	15.566904192274167
33	30.163369773902442	26.534417634907104	23.99612826628411	19.306084324906344
34	19.876848216200127	27.31458524138531	27.148801160046474	25.65976538236809
35	20.772082255429797	25.424253744448382	31.2236756369363	22.57998836318552
36	30.83895832581165	26.680062020438843	24.81728752191113	17.663692131838378
37	19.834481173191314	36.55794423809443	26.907616022454288	16.69995856625997
38	19.521922918507176	36.18209330524583	23.308210290844226	20.987773485402773
39	20.89994476320016	33.840165970741936	26.022869361407086	19.237019904650822
40	25.5464673131509	25.135470155058464	26.20233369953195	23.115728832258682
41	16.355275040703063	24.74456357156383	27.77909995699438	31.121061430738727
42	19.74348413298978	25.731654272157535	24.905853062253026	29.61900853259966
43	19.994886482113372	25.748576528756416	27.452173748594213	26.804363240536
44	18.08905724364685	29.8331278838755	30.949284561867056	21.1285303106106
45	16.72400339820526	39.45567805566464	23.61433366711195	20.205984879018146
46	20.596400250419926	34.90184722763579	24.936725742289013	19.565026779655273
47	20.681478184902545	28.069258940244296	26.659455673143544	24.589807201709615
48	20.902474505478366	26.22998894035973	32.58858211875003	20.278954435411872
49	20.60111588651134	26.653020794727134	33.32618619738228	19.419677121379245
50	19.087544064794805	35.85804068758887	25.923546276231647	19.130868971384686
51	17.945746115556172	34.7008432392392	25.721265136393633	21.632145508810993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8198.0
1	8573.5
2	8949.0
3	20506.5
4	32064.0
5	23595.0
6	15126.0
7	15230.5
8	15335.0
9	16312.5
10	17290.0
11	17692.0
12	18094.0
13	18194.0
14	18294.0
15	17592.0
16	16890.0
17	15816.5
18	14743.0
19	15921.0
20	17099.0
21	18896.5
22	20694.0
23	20558.0
24	20422.0
25	22320.0
26	31414.5
27	38611.0
28	47786.5
29	56962.0
30	67344.0
31	77726.0
32	87654.5
33	97583.0
34	109886.0
35	122189.0
36	128369.5
37	134550.0
38	158883.5
39	183217.0
40	250046.0
41	316875.0
42	384233.5
43	451592.0
44	445963.5
45	440335.0
46	407581.0
47	374827.0
48	345490.0
49	316153.0
50	272461.5
51	228770.0
52	204128.0
53	179486.0
54	166383.0
55	153280.0
56	140274.5
57	127269.0
58	126088.0
59	124907.0
60	118332.5
61	111758.0
62	101260.5
63	90763.0
64	76658.0
65	62553.0
66	53279.5
67	44006.0
68	37983.0
69	31960.0
70	27202.0
71	22444.0
72	19505.0
73	16566.0
74	12900.5
75	7356.0
76	5477.0
77	3967.5
78	2458.0
79	1906.0
80	1354.0
81	1000.5
82	647.0
83	510.5
84	374.0
85	283.0
86	192.0
87	105.5
88	19.0
89	12.0
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4071561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.163663468225122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.80470869681848	11.949680823900689
2	8.379623043964633	2.5413156765852785
3	3.078186250379838	1.4002974117983287
4	1.5815001636160584	0.9592534502406751
5	0.9601090825889861	0.7279385510582873
6	0.6906752172355572	0.6283899936001962
7	0.4824683758805396	0.5121191660139551
8	0.4009999079363949	0.4864502123789399
9	0.2783559424157459	0.37988062516557186
>10	2.684863582191801	9.657332403935776
>50	1.0937928999574	12.124103644328233
>100	1.4715392707254538	47.884888663705254
>500	0.08260065335902711	7.653407436965663
>1k	0.010073250409637451	2.2370108269088576
>5k	3.357750136545817E-4	0.39049867503448193
>10k+	1.6788750682729086E-4	0.4674324383798242
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18361	0.450957261846255	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8204	0.20149520048944372	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7135	0.17523991412630194	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4646	0.11410856917039927	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4632	0.11376472070540021	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4286	0.1052667514989951	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02927624073420489	0.0
2	0.0	0.0	0.0	0.10074760024472186	0.0
3	0.0	0.0	0.0	0.14056034037068338	0.0
4	0.0	0.0	0.0	0.1990391400251648	0.0
5	0.0	0.0	0.0	0.3685073120604112	0.0
6	0.0	0.0	0.0	0.5205128941946344	0.0
7	0.0	0.0	0.0	0.6241832063918482	0.0
8	0.0	0.0	0.0	0.8876448123950494	0.0
9	0.0	0.0	0.0	0.9830382008276433	0.0
10	0.0	0.0	0.0	1.163877932812501	0.0
11	4.9121209285578674E-5	0.0	0.0	1.353485800654835	0.0
12	4.9121209285578674E-5	0.0	0.0	1.5202523061793745	0.0
13	7.368181392836801E-5	0.0	0.0	1.5874255598774032	0.0
14	7.368181392836801E-5	0.0	0.0	1.6104634070323396	0.0
15	9.824241857115735E-5	0.0	0.0	1.6548689802265029	0.0
16	1.2280302321394668E-4	0.0	0.0	1.7536517320998015	0.0
17	1.4736362785673602E-4	0.0	0.0	1.8767986037787472	0.0
18	1.4736362785673602E-4	0.0	0.0	2.0598242295768134	0.0
19	1.4736362785673602E-4	0.0	0.0	2.134242861644465	0.0
20	1.4736362785673602E-4	0.0	0.0	2.2196646445920862	0.0
21	1.964848371423147E-4	0.0	0.0	2.335467895482838	0.0
22	1.964848371423147E-4	0.0	0.0	2.458835812603569	0.0
23	1.964848371423147E-4	0.0	0.0	2.5992487893463956	0.0
24	1.964848371423147E-4	0.0	0.0	2.7149046766092906	0.0
25	1.964848371423147E-4	0.0	0.0	2.806147322857253	0.0
26	1.964848371423147E-4	0.0	0.0	2.905151120172337	0.0
27	2.2104544178510403E-4	0.0	0.0	2.9929798423749516	0.0
28	2.2104544178510403E-4	0.0	0.0	3.095078275875027	0.0
29	2.2104544178510403E-4	0.0	0.0	3.207516723929716	0.0
30	2.2104544178510403E-4	0.0	0.0	3.346799912858975	0.0
31	2.2104544178510403E-4	0.0	0.0	3.4817604353711022	0.0
32	2.2104544178510403E-4	0.0	0.0	3.6089106856068227	0.0
33	2.2104544178510403E-4	0.0	0.0	3.7319101936579115	0.0
34	2.2104544178510403E-4	0.0	0.0	3.8669689585886102	0.0
35	2.2104544178510403E-4	0.0	0.0	4.036805539693498	0.0
36	2.4560604642789337E-4	0.0	0.0	4.188197106711652	0.0
37	2.4560604642789337E-4	0.0	0.0	4.3379431132187385	0.0
38	2.4560604642789337E-4	0.0	0.0	4.477299983961925	0.0
39	2.7016665107068273E-4	0.0	0.0	4.628986278235792	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGC	20	7.0354046E-4	45.0	14
TATATCG	20	7.0354046E-4	45.0	40
CAATTCG	50	2.1827873E-11	45.0	1
TACGATC	25	3.8925595E-5	45.0	11
ACGTCCA	20	7.0354046E-4	45.0	14
TACGTCC	25	3.8925595E-5	45.0	31
GCGTGTT	60	0.0	44.999996	44
TCACGAC	310	0.0	42.09677	25
CGGTCTA	305	0.0	42.049183	31
ATATACG	75	0.0	42.0	1
GTCGATA	70	0.0	41.785713	8
TTACGAG	140	0.0	41.785713	1
ACGTAAG	130	0.0	41.53846	1
TACGGGA	1015	0.0	40.788177	4
TAACGGG	980	0.0	40.408165	3
TATAGCG	140	0.0	40.17857	1
CTAACGG	135	0.0	40.0	2
TCATCGA	850	0.0	39.441174	16
CGGGAAC	400	0.0	39.375	6
ATCGGAT	40	3.4611185E-7	39.375	16
>>END_MODULE