FastQCFastQC Report
Thu 26 May 2016
SRR1545242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545242_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1345361
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55320.4111907510326225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26910.20002066359884074No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23950.17801913389789062No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13470.10012182603776978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAT351.2121382E-745.0000049
CCACGCG207.033717E-445.01
GTACCGC253.891163E-544.99999630
CTATACG253.891163E-544.9999961
CTAAGCG253.891163E-544.9999961
TACGGAC253.891163E-544.99999635
CCCGTAT253.891163E-544.99999634
CACGACG1100.042.95454426
AATGCGG2000.042.7499962
AATACGG1450.041.896552
ACGGGCT2500.041.3999985
GAGTACG603.6379788E-1241.2499961
CGGGCTA556.184564E-1140.9090926
GTCGTTT1100.040.9090929
CGTAAGG1650.040.9090922
ATGGGCG4350.040.862075
GCTACGA1050.040.71428710
TGTACCG501.0822987E-940.4999961
ATAGGGC4850.040.3608254
CGGGTAC1450.040.3448266