FastQCFastQC Report
Thu 26 May 2016
SRR1545241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545241_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2179441
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116300.5336230712370741No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51900.23813445741362121No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG27720.12718857725444277No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG23020.10562341444434606No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT22780.1045222146412773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGA207.0346793E-445.00000426
GACTACG650.044.9999961
TGTAACG1050.040.7142871
ACTACGG1500.039.0000042
ACGGGTA2450.038.571435
ACCCGCT9800.038.5714334
AGGGCGA19650.038.473286
AAGGGCG8450.038.3431935
CGTTTTT81350.038.0854341
CGTATGG2600.038.076922
TAACGGG6150.038.048783
ATAGGGC9700.038.0412374
TACGGGC3850.037.9870154
CGTAAGG2850.037.8947372
CGGTAGT4400.037.84090812
GGGCGAT37100.037.6010787
CGGTAAG301.1402575E-437.5000041
TATCGCG601.5643309E-1037.5000041
ACGGTCT1500.037.50000430
AGGGATC12200.037.4385266