FastQCFastQC Report
Thu 26 May 2016
SRR1545240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545240_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3617850
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA152000.4201390328510027No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123430.3411694791105215No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC119670.33077656619262824No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47820.13217795099299307No Hit
GGATATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA40710.11252539491687051No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA406.8212103E-945.00000437
TGAACGT207.035297E-445.00000444
CGGGTAT4900.045.06
GCGCGTT351.2129021E-745.015
TAGACGT453.8562575E-1045.03
GACGTAG1100.045.01
TCGATTG1900.045.01
CGCGACA253.892473E-545.029
ATCACGC253.892473E-545.038
GCCGAAT502.1827873E-1145.029
ATGACCG950.045.018
ACGCTAT453.8562575E-1045.045
ACATCGC551.8189894E-1245.032
GTGCGTA253.892473E-545.016
TGTCGGT351.2129021E-745.012
TACCGGC253.892473E-545.025
CCCGTGC302.1668002E-644.99999642
GTGCTAC302.1668002E-644.9999962
AGTCGAC302.1668002E-644.99999614
CGATATC2400.044.06249610
Produced by FastQC (version 0.11.5)