##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545240_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3617850
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.169159860137928	31.0	31.0	33.0	28.0	34.0
2	31.415406111364483	31.0	31.0	34.0	30.0	34.0
3	31.50976933814282	31.0	31.0	34.0	30.0	34.0
4	35.252383321586024	37.0	35.0	37.0	33.0	37.0
5	35.093937836007576	37.0	35.0	37.0	32.0	37.0
6	35.08278535594345	37.0	35.0	37.0	32.0	37.0
7	35.79456666252056	37.0	35.0	37.0	35.0	37.0
8	35.91645894661194	37.0	35.0	37.0	35.0	37.0
9	37.616291167406054	39.0	37.0	39.0	35.0	39.0
10	36.80094780048924	39.0	37.0	39.0	32.0	39.0
11	36.337433835012504	38.0	35.0	39.0	32.0	39.0
12	34.77129068369335	35.0	34.0	39.0	30.0	39.0
13	33.97006979283276	35.0	33.0	39.0	25.0	39.0
14	34.599734372624624	35.0	33.0	40.0	25.0	41.0
15	35.32539795735036	35.0	33.0	40.0	30.0	41.0
16	35.444844313611675	35.0	34.0	40.0	30.0	41.0
17	35.5662280083475	35.0	34.0	40.0	31.0	41.0
18	35.68445651422806	36.0	34.0	39.0	31.0	41.0
19	35.57230454551736	36.0	34.0	40.0	30.0	41.0
20	35.38244648064458	35.0	34.0	40.0	30.0	41.0
21	35.157403430214075	35.0	34.0	39.0	29.0	41.0
22	35.045919814254326	35.0	34.0	39.0	29.0	41.0
23	35.057790953190434	35.0	34.0	39.0	29.0	41.0
24	34.90450433268378	35.0	34.0	39.0	29.0	41.0
25	34.88973478723551	35.0	34.0	39.0	29.0	41.0
26	34.735306051936924	35.0	34.0	39.0	29.0	41.0
27	34.63268681675581	35.0	34.0	39.0	29.0	41.0
28	34.78031897397626	35.0	34.0	39.0	29.0	41.0
29	34.86459748192988	35.0	34.0	39.0	29.0	41.0
30	34.811220752656965	35.0	34.0	39.0	29.0	41.0
31	34.5942230882983	35.0	34.0	39.0	29.0	41.0
32	34.390390148845306	35.0	34.0	39.0	28.0	41.0
33	34.199492792680736	35.0	33.0	39.0	27.0	41.0
34	34.09626933123264	35.0	33.0	39.0	27.0	41.0
35	33.91146260900811	35.0	33.0	39.0	25.0	41.0
36	33.620423455919955	35.0	33.0	38.0	23.0	41.0
37	33.43398814212861	35.0	33.0	38.0	23.0	41.0
38	33.59253423994914	35.0	33.0	38.0	24.0	41.0
39	33.647527122462236	35.0	33.0	38.0	24.0	41.0
40	33.42839531766104	35.0	32.0	38.0	23.0	41.0
41	33.54334701549263	35.0	33.0	38.0	23.0	41.0
42	33.504998272454635	35.0	33.0	38.0	23.0	41.0
43	33.33568832317537	35.0	33.0	38.0	23.0	41.0
44	33.328455298036125	35.0	33.0	38.0	23.0	40.0
45	33.266569647718946	35.0	32.0	38.0	23.0	40.0
46	33.23749215694404	35.0	32.0	38.0	23.0	40.0
47	33.25469823237558	35.0	32.0	38.0	23.0	40.0
48	33.30653786088423	35.0	33.0	38.0	23.0	40.0
49	33.36215514739417	35.0	33.0	38.0	24.0	40.0
50	33.08953052227152	35.0	32.0	38.0	24.0	40.0
51	32.86376217919483	35.0	32.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	0.0
11	3.0
12	6.0
13	12.0
14	34.0
15	130.0
16	382.0
17	1121.0
18	2543.0
19	4880.0
20	8407.0
21	13429.0
22	19621.0
23	28268.0
24	39607.0
25	53729.0
26	68421.0
27	80976.0
28	91686.0
29	105327.0
30	126586.0
31	156214.0
32	197592.0
33	262767.0
34	468719.0
35	704716.0
36	207022.0
37	228005.0
38	304553.0
39	442635.0
40	457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.073565791837694	24.7291899885291	29.854858548585483	14.342385671047722
2	29.251959036444298	26.414997857843748	31.638818635377362	12.694224470334593
3	27.150904542753292	26.542587448346396	32.82236687535415	13.484141133546167
4	23.911687880923754	29.030169852260318	32.358693699296545	14.699448567519383
5	22.442914990947664	32.58902386776677	31.69293917658278	13.275121964702793
6	20.59430324640325	40.674571914258465	27.952789640255954	10.778335199082328
7	86.45543623975566	5.648658733778349	5.986069074173888	1.9098359522921073
8	88.99238498002957	3.7047141257929432	5.509377116243073	1.7935237779344086
9	86.38307281949224	4.386279143690313	6.944234835606783	2.2864132012106637
10	64.39758420056111	17.441159804856476	10.844838785466507	7.316417209115911
11	59.97434940641541	15.399560512459058	16.034495625855136	8.591594455270396
12	53.29565902400597	19.76306369805271	18.35518332711417	8.586093950827149
13	18.660143455367137	54.254156474149006	18.132979532042512	8.952720538441339
14	10.631784070649696	54.697320231629284	24.20788589908371	10.463009798637312
15	8.545545006011858	21.52242353884213	59.51775225617425	10.414279198971766
16	10.065840208963888	14.894702654891717	57.3789128902525	17.660544245891895
17	10.596459223019195	15.532512403775723	28.26347139875893	45.60755697444615
18	17.642964744254183	20.94158132592562	42.60408806335254	18.811365866467654
19	33.95773733018229	20.681592658623217	24.791630388213996	20.5690396229805
20	35.55777602719847	20.485481708749674	24.931160772281878	19.025581491769973
21	18.44739278853463	33.79482289204915	28.847934546761202	18.90984977265503
22	20.799010462014735	24.133255939300966	20.69071409815222	34.377019500532086
23	13.771300634354658	35.27280014373177	23.115496772945257	27.84040244896831
24	14.584850118164102	20.92300675815747	50.42013903285101	14.072004090827425
25	11.246126843290904	23.70764404273256	45.52651436626726	19.519714747709273
26	11.33548930994928	41.7999640670564	26.88102049559821	19.983526127396107
27	12.460079881697693	46.446066033694045	25.744765537543017	15.349088547065245
28	9.637491880536784	32.36104316099341	43.403983028594325	14.597481929875478
29	9.69993228022168	23.862819077628977	42.90669872990865	23.530549912240694
30	14.804649170087206	39.21483754163385	30.485398786572137	15.495114501706814
31	33.70775460563594	28.139834432052186	23.202592700084303	14.949818262227566
32	36.48907500310958	22.836795334245476	26.223585831363934	14.45054383128101
33	34.65602498721616	26.723164310294788	20.973782771535582	17.647027930953467
34	17.78177646945009	25.980292162472185	25.69476346448858	30.543167903589147
35	17.338640352695663	23.79742112027862	35.66610003178683	23.197838495238884
36	37.978965407631605	23.038434429288113	23.678538358417292	15.304061804662991
37	17.096784001547878	39.85405696753597	28.45797918653344	14.591179844382712
38	16.54369307738021	40.35167295493179	22.02059234075487	21.08404162693312
39	18.33436986055254	38.591953784706384	25.72326105283525	17.350415301905826
40	27.579805685697306	23.023259670798954	24.283649128626116	25.11328551487762
41	13.605428638556049	22.001824287905798	27.235374600937018	37.15737247260113
42	18.072114653730807	21.4178863136946	24.611191729894827	35.89880730267977
43	18.8247716185027	22.396312727172216	27.197036914189365	31.581878740135714
44	14.778031151098029	30.25813673867076	32.90924167668643	22.054590433544785
45	12.66116616222342	48.503696947081835	19.32446066033694	19.51067623035781
46	20.48426551681247	40.121038738477274	22.901806321434	16.492889423276257
47	18.657821634396118	28.46289923573393	25.08097350636428	27.798305623505676
48	20.40413505258648	23.861271196981633	36.77054051439391	18.964053236037977
49	21.772765592824467	22.68703788161477	37.20389181419904	18.33630471136172
50	18.74806860428155	40.83215169230344	23.936067001119447	16.483712702295563
51	15.538759207816796	39.4583799770582	22.401315698550246	22.601545116574762
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16498.0
1	13309.5
2	10121.0
3	15922.5
4	21724.0
5	16131.0
6	10538.0
7	11431.5
8	12325.0
9	13944.5
10	15564.0
11	16901.0
12	18238.0
13	18130.0
14	18022.0
15	17013.0
16	16004.0
17	15321.0
18	14638.0
19	12807.0
20	10976.0
21	11123.0
22	11270.0
23	11517.5
24	11765.0
25	11686.0
26	16477.5
27	21348.0
28	25949.5
29	30551.0
30	35186.5
31	39822.0
32	51206.5
33	62591.0
34	76912.0
35	91233.0
36	96444.5
37	101656.0
38	134484.5
39	167313.0
40	264784.5
41	362256.0
42	441497.0
43	520738.0
44	517723.5
45	514709.0
46	452816.5
47	390924.0
48	357299.0
49	323674.0
50	282073.0
51	240472.0
52	197906.0
53	155340.0
54	127118.5
55	98897.0
56	83894.0
57	68891.0
58	65840.5
59	62790.0
60	55561.0
61	48332.0
62	43180.0
63	38028.0
64	34106.0
65	30184.0
66	24491.0
67	18798.0
68	14804.5
69	10811.0
70	9166.5
71	7522.0
72	6773.5
73	6025.0
74	4398.0
75	2295.0
76	1819.0
77	1145.5
78	472.0
79	329.5
80	187.0
81	270.0
82	353.0
83	195.0
84	37.0
85	24.0
86	11.0
87	6.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3617850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.079229889804592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3542438096833	10.890881815485251
2	9.514964815892197	2.6792675407269697
3	3.6560545770757353	1.5442329864096476
4	1.9487318118402295	1.0974657268989607
5	1.1633352646744344	0.8189432315134016
6	0.8219170788770949	0.6943175702320366
7	0.5848266892427192	0.576373658247909
8	0.4291714155925023	0.48339224178077667
9	0.3320663378623941	0.4207714478487153
>10	2.1083520816208168	6.079404733060775
>50	0.6061662506521319	6.208599522861266
>100	1.171843554227057	35.94460237339605
>500	0.28024114367635666	25.85784370538102
>1k	0.02747462192905457	5.571805802673509
>5k	0.0	0.0
>10k+	6.105471539789905E-4	1.1320976434837384
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15200	0.4201390328510027	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12343	0.3411694791105215	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11967	0.33077656619262824	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4782	0.13217795099299307	No Hit
GGATATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	4071	0.11252539491687051	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.76407258454607E-5	0.0	0.0	0.07847202067526293	0.0
2	2.76407258454607E-5	0.0	0.0	0.2605138410934671	0.0
3	2.76407258454607E-5	0.0	0.0	0.35236397307793305	0.0
4	2.76407258454607E-5	0.0	0.0	0.4651104938015672	0.0
5	2.76407258454607E-5	0.0	0.0	0.7887833934519121	0.0
6	2.76407258454607E-5	0.0	0.0	1.0459527067180785	0.0
7	2.76407258454607E-5	0.0	0.0	1.258261121937062	0.0
8	2.76407258454607E-5	0.0	0.0	1.761571098857056	0.0
9	2.76407258454607E-5	0.0	0.0	1.9337175394225852	0.0
10	2.76407258454607E-5	0.0	0.0	2.2288099285487237	0.0
11	2.76407258454607E-5	0.0	0.0	2.5291264148596544	0.0
12	2.76407258454607E-5	0.0	0.0	2.8024379120195695	0.0
13	8.292217753638211E-5	0.0	0.0	2.9045427532927013	0.0
14	8.292217753638211E-5	0.0	0.0	2.935942617853145	0.0
15	8.292217753638211E-5	0.0	0.0	2.9967245739873127	0.0
16	8.292217753638211E-5	0.0	0.0	3.1425017620962725	0.0
17	8.292217753638211E-5	0.0	0.0	3.313100322014456	0.0
18	1.105629033818428E-4	0.0	0.0	3.617811683734815	0.0
19	1.105629033818428E-4	0.0	0.0	3.712978702820736	0.0
20	1.382036292273035E-4	0.0	0.0	3.8118772198957944	0.0
21	1.382036292273035E-4	0.0	0.0	3.960833091476982	0.0
22	1.382036292273035E-4	0.0	0.0	4.1034039553878685	0.0
23	1.382036292273035E-4	0.0	0.0	4.2922177536382105	0.0
24	1.6584435507276422E-4	0.0	0.0	4.418701715107039	0.0
25	1.9348508091822491E-4	0.0	0.0	4.518899346296834	0.0
26	1.9348508091822491E-4	0.0	0.0	4.637118730737869	0.0
27	1.9348508091822491E-4	0.0	0.0	4.732893845792391	0.0
28	2.211258067636856E-4	0.0	0.0	4.8343905910969225	0.0
29	2.211258067636856E-4	0.0	0.0	4.94766228561162	0.0
30	2.211258067636856E-4	0.0	0.0	5.129980513288279	0.0
31	2.211258067636856E-4	0.0	0.0	5.296681730862252	0.0
32	2.211258067636856E-4	0.0	0.0	5.422308829829872	0.0
33	2.211258067636856E-4	0.0	0.0	5.545310059842172	0.0
34	2.4876653260914633E-4	0.0	0.0	5.686775294719239	0.0
35	2.4876653260914633E-4	0.0	0.0	5.910167641002253	0.0
36	2.76407258454607E-4	0.0	0.0	6.058736542421604	0.0
37	2.76407258454607E-4	0.0	0.0	6.200865154718963	0.0
38	2.76407258454607E-4	0.0	0.0	6.3379355141866025	0.0
39	2.76407258454607E-4	0.0	0.0	6.479677156322125	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	40	6.8212103E-9	45.000004	37
TGAACGT	20	7.035297E-4	45.000004	44
CGGGTAT	490	0.0	45.0	6
GCGCGTT	35	1.2129021E-7	45.0	15
TAGACGT	45	3.8562575E-10	45.0	3
GACGTAG	110	0.0	45.0	1
TCGATTG	190	0.0	45.0	1
CGCGACA	25	3.892473E-5	45.0	29
ATCACGC	25	3.892473E-5	45.0	38
GCCGAAT	50	2.1827873E-11	45.0	29
ATGACCG	95	0.0	45.0	18
ACGCTAT	45	3.8562575E-10	45.0	45
ACATCGC	55	1.8189894E-12	45.0	32
GTGCGTA	25	3.892473E-5	45.0	16
TGTCGGT	35	1.2129021E-7	45.0	12
TACCGGC	25	3.892473E-5	45.0	25
CCCGTGC	30	2.1668002E-6	44.999996	42
GTGCTAC	30	2.1668002E-6	44.999996	2
AGTCGAC	30	2.1668002E-6	44.999996	14
CGATATC	240	0.0	44.062496	10
>>END_MODULE