FastQCFastQC Report
Thu 26 May 2016
SRR1545239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545239_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences937762
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31230.333026930073942No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20110.21444673595219255No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18980.2023967701826263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG453.8380676E-1045.0000041
TCCGTTG453.8380676E-1045.0000041
CCGACGG453.8380676E-1045.0000042
CTAGACG207.0326217E-445.01
AACGTAG253.8902544E-545.01
CGGGTCC253.8902544E-545.06
CGTATTA207.0326217E-445.010
TCCGCAA207.0326217E-445.032
CTCCGAA207.0326217E-445.027
CACGGGT551.8189894E-1245.04
CGACGGT406.8121153E-945.028
CTATACG253.8902544E-545.01
CACGCTA207.0326217E-445.017
GGTCCGC207.0326217E-445.030
CCGTGCA207.0326217E-445.025
CGCGAGT207.0326217E-445.021
ACGGGTC750.045.05
AGGTTCG253.8902544E-545.01
GTCCGCA207.0326217E-445.031
CGGTGCC207.0326217E-445.027