FastQCFastQC Report
Thu 26 May 2016
SRR1545238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545238_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3713215
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509541.37223403438799No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71120.1915321358984061No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT64430.17351540376735525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT372750.043.4849131
AGGGCGA27900.039.9193536
CGGTCTA2000.039.37531
GGGCGAT52350.039.197717
GCTACGA8700.038.79310210
CTACGAA8650.038.7572311
GCGATCG3000.038.2500049
AGGGATC49800.037.951816
ATACTAT57500.037.64347545
GTTTTTT442150.037.555132
GCCCTAC58750.037.53191426
CCCTACA58700.037.48722527
ACGGGAT17950.037.4791075
AGGGATG83350.037.4685066
TAGGGAT67300.037.3439835
AAGGGAT71050.037.3363845
GCCCTAG59750.037.2050220
CGTTGAT10550.037.1090125
TAAGGGA49550.037.0534824
TACGGGA12900.036.9767464