##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545238_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3713215
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14435011169566	31.0	31.0	33.0	30.0	34.0
2	31.454007376357147	31.0	31.0	34.0	30.0	34.0
3	31.489905917109567	31.0	31.0	34.0	30.0	34.0
4	35.30168438940379	37.0	35.0	37.0	33.0	37.0
5	35.17381891433703	37.0	35.0	37.0	32.0	37.0
6	35.168706094314494	37.0	35.0	37.0	32.0	37.0
7	35.84095696047765	37.0	35.0	37.0	35.0	37.0
8	35.92403025410594	37.0	35.0	37.0	35.0	37.0
9	37.5443756959939	39.0	37.0	39.0	35.0	39.0
10	36.74741591855037	39.0	37.0	39.0	32.0	39.0
11	36.43034809457573	39.0	35.0	39.0	32.0	39.0
12	36.18572557743088	38.0	35.0	39.0	31.0	39.0
13	36.0682958029632	38.0	35.0	39.0	31.0	39.0
14	37.012910375510174	39.0	35.0	41.0	31.0	41.0
15	37.23722461532661	39.0	35.0	41.0	32.0	41.0
16	37.00547288535676	39.0	35.0	41.0	31.0	41.0
17	37.04833681863291	39.0	35.0	41.0	31.0	41.0
18	36.97150420861706	39.0	35.0	40.0	31.0	41.0
19	36.83309988783305	38.0	35.0	40.0	31.0	41.0
20	36.66475547470319	38.0	35.0	40.0	31.0	41.0
21	36.577889510841686	38.0	35.0	40.0	31.0	41.0
22	36.500903933653184	38.0	35.0	40.0	31.0	41.0
23	36.38679392386382	38.0	35.0	40.0	30.0	41.0
24	36.17443751573771	38.0	35.0	40.0	30.0	41.0
25	36.1362547011148	38.0	35.0	40.0	30.0	41.0
26	36.04218635333532	38.0	35.0	40.0	30.0	41.0
27	35.99423572295167	38.0	35.0	40.0	30.0	41.0
28	35.93886887777842	38.0	35.0	40.0	30.0	41.0
29	35.851539164847715	38.0	34.0	40.0	30.0	41.0
30	35.704664556186486	38.0	34.0	40.0	29.0	41.0
31	35.54031775698418	38.0	34.0	40.0	28.0	41.0
32	35.25329532494079	38.0	34.0	40.0	26.0	41.0
33	34.90243791431415	38.0	34.0	40.0	24.0	41.0
34	34.56301049090882	38.0	33.0	40.0	23.0	41.0
35	34.29395739271763	38.0	33.0	40.0	22.0	41.0
36	34.11202529344517	37.0	33.0	40.0	21.0	41.0
37	33.946592912072155	37.0	33.0	40.0	20.0	41.0
38	33.93794272618203	37.0	33.0	40.0	20.0	41.0
39	33.90221061802239	37.0	33.0	40.0	20.0	41.0
40	33.79369468237094	37.0	33.0	40.0	18.0	41.0
41	33.70922071574094	37.0	33.0	40.0	18.0	41.0
42	33.614057629305066	37.0	33.0	40.0	18.0	41.0
43	33.50455333181623	37.0	32.0	40.0	18.0	41.0
44	33.429801398518535	37.0	32.0	40.0	18.0	41.0
45	33.38475795234049	37.0	32.0	40.0	18.0	41.0
46	33.29011166872912	36.0	32.0	40.0	18.0	41.0
47	33.307159967844576	36.0	32.0	40.0	18.0	41.0
48	33.23764958398584	36.0	32.0	40.0	18.0	41.0
49	33.180731791722266	36.0	32.0	40.0	18.0	41.0
50	33.038216208864824	36.0	32.0	40.0	18.0	41.0
51	32.897485063482726	36.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	6.0
13	17.0
14	36.0
15	176.0
16	491.0
17	1316.0
18	3198.0
19	5906.0
20	9831.0
21	15251.0
22	22156.0
23	31565.0
24	46019.0
25	65858.0
26	87381.0
27	96020.0
28	95483.0
29	98287.0
30	109820.0
31	128319.0
32	154249.0
33	192138.0
34	273716.0
35	352615.0
36	296147.0
37	364649.0
38	514313.0
39	747953.0
40	298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.064158687283122	22.359976462445616	25.487212563775596	21.088652286495666
2	36.951482744737376	23.62211722186838	27.49816533650758	11.92823469688666
3	26.62135103946311	24.696738540590836	36.60332084191193	12.07858957803413
4	23.99753313503258	27.27986394539503	35.327768524041836	13.394834395530559
5	21.11897641262356	31.213921089944968	34.109632757596856	13.557469739834618
6	19.86903532383662	38.9884237783161	31.666843961365014	9.475696936482267
7	82.71748336683979	3.3823788819123046	12.308605884657904	1.5915318665900033
8	83.57840846813342	2.9028214094793863	12.15566564284589	1.363104479541314
9	78.67761495092527	5.583571110210424	13.433426289616948	2.30538764924735
10	36.666042768867406	34.070502246705345	19.459498036068474	9.803956948358767
11	25.531756173558495	23.593058845232502	35.05261612914954	15.822568852059469
12	26.84493087526577	21.788827202303125	35.88130501465711	15.48493690777399
13	20.464556994410504	29.101358256928293	34.086822335900294	16.34726241276091
14	16.452454274799603	31.085407120244856	34.87223336111698	17.58990524383856
15	15.590209562333449	26.308414675692088	41.82090183304764	16.28047392892682
16	18.527502447340108	23.318849029749153	40.020332784393034	18.133315738517698
17	18.186584940543437	23.11789109976126	35.52379811026294	23.171725849432363
18	19.20758695631683	24.764577327194896	38.00741406032239	18.020421656165883
19	22.940928548441175	26.70039305561353	32.806180089221876	17.552498306723418
20	24.849328681479527	24.5773541257374	33.68978095801078	16.883536234772293
21	21.178385846227595	26.124450105905527	34.89350872491897	17.803655322947904
22	20.237018325090254	24.17950482264022	33.69845268857311	21.88502416369642
23	17.89185382478526	27.437732530973836	34.69069256695344	19.979721077287472
24	17.969414644721624	25.100916591148103	40.23763773441613	16.692031029714144
25	18.08974163898401	28.249535779641093	35.842605397209695	17.818117184165207
26	18.408333479208718	29.93640282073621	32.849700327075055	18.805563372980018
27	16.66049501577474	29.26016942191605	34.68711076519943	19.392224797109783
28	16.792213755465276	27.075431937014148	38.04931844776023	18.083035859760344
29	17.26520010287581	24.998929499099837	37.82991289219719	19.90595750582716
30	18.392605868499402	27.806361872393598	36.287341293192014	17.513690965914982
31	21.664595236203667	27.839675321789876	33.28282903090718	17.212900411099277
32	22.699816735632062	26.722745653025747	32.34251181253981	18.234925798802383
33	22.447420900755812	28.3351489208139	30.998609022100794	18.218821156329486
34	18.902056573616125	27.481252768827012	32.28636101060671	21.33032964695015
35	19.392359451311062	27.811559524562945	33.13066978346258	19.665411240663413
36	22.563034997973457	29.082722115471366	29.810958966825247	18.543283919729937
37	19.498197653515888	31.746559248521834	31.098899471212953	17.656343626749326
38	19.232767291955895	30.269456522178217	32.02973703381032	18.468039152055564
39	19.330041486959416	31.6141672378249	30.1710243010437	18.884766974171978
40	21.876971842459973	28.114773855001662	31.45977273063908	18.548481571899284
41	18.319596360566248	28.370239805667058	31.438093404233257	21.872070429533437
42	19.213727187895127	28.804768913192476	29.811901546234193	22.169602352678204
43	19.42174099803001	28.17310605499547	31.167034497059827	21.238118449914698
44	19.36486306341001	29.145928797551445	32.12504527747518	19.36416286156336
45	18.5376553741165	32.388913650300346	29.00860844308773	20.064822532495423
46	18.962247001587574	31.63913212674192	30.281575400293274	19.11704547137723
47	18.771280413334537	30.156697094027685	31.03580051249389	20.036221980143893
48	19.484624510027025	27.77310228467783	32.69544047409051	20.04683273120463
49	18.82605774241459	28.591018834083133	32.988205638510024	19.59471778499225
50	18.369687723441817	31.457268162495307	31.37677188097107	18.79627223309181
51	17.814212212328133	30.696471925272306	31.419107161853006	20.07020870054656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7878.0
1	7025.5
2	6173.0
3	44838.5
4	83504.0
5	60862.5
6	38221.0
7	37731.5
8	37242.0
9	37928.0
10	38614.0
11	38798.5
12	38983.0
13	37604.5
14	36226.0
15	34674.0
16	33122.0
17	31839.0
18	30556.0
19	29296.5
20	28037.0
21	28118.5
22	28200.0
23	31399.5
24	34599.0
25	37994.0
26	49299.5
27	57210.0
28	66698.5
29	76187.0
30	88673.5
31	101160.0
32	118281.0
33	135402.0
34	152679.0
35	169956.0
36	181922.5
37	193889.0
38	207809.0
39	221729.0
40	236318.0
41	250907.0
42	263249.0
43	275591.0
44	276720.5
45	277850.0
46	263954.0
47	250058.0
48	241513.5
49	232969.0
50	216100.5
51	199232.0
52	182545.5
53	165859.0
54	150535.5
55	135212.0
56	123989.0
57	112766.0
58	105047.5
59	97329.0
60	90467.5
61	83606.0
62	73148.0
63	62690.0
64	52473.0
65	42256.0
66	35301.0
67	28346.0
68	24291.5
69	20237.0
70	17538.5
71	14840.0
72	12866.0
73	10892.0
74	9140.0
75	5442.5
76	3497.0
77	2614.0
78	1731.0
79	1359.0
80	987.0
81	685.0
82	383.0
83	299.5
84	216.0
85	132.5
86	49.0
87	34.5
88	20.0
89	13.0
90	6.0
91	5.0
92	4.0
93	5.5
94	7.0
95	7.5
96	8.0
97	4.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3713215.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.702718448622452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4584809230611	17.02762321379811
2	5.679555312265807	2.4652357971096555
3	2.3033140819326037	1.4996453105679182
4	1.4724025091681117	1.2782054839808306
5	1.1133617442404067	1.2081488233358373
6	0.8454049847986996	1.1008551816088874
7	0.6954433896633729	1.056510845797387
8	0.5527356680962429	0.9596693264963189
9	0.4825028292294314	0.9424460748087098
>10	6.303274278345368	32.89335366653428
>50	1.6944024873077161	25.665033578517267
>100	0.3947898764792691	11.374259285898624
>500	0.0029304133669594335	0.43630949389125673
>1k	0.0010192742145945389	0.30894771374908647
>5k	2.548185536486347E-4	0.3748130546736657
>10k+	1.2740927682431736E-4	1.4089431492321625
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50954	1.37223403438799	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7112	0.1915321358984061	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	6443	0.17351540376735525	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6930840255681397E-5	0.0	0.0	0.014731169619857725	0.0
2	2.6930840255681397E-5	0.0	0.0	0.044974503226987934	0.0
3	2.6930840255681397E-5	0.0	0.0	0.08065786656576579	0.0
4	2.6930840255681397E-5	0.0	0.0	0.11114357773519713	0.0
5	2.6930840255681397E-5	0.0	0.0	0.20408190745755364	0.0
6	2.6930840255681397E-5	0.0	0.0	0.3662324966370113	0.0
7	2.6930840255681397E-5	0.0	0.0	0.48521294888661176	0.0
8	2.6930840255681397E-5	0.0	0.0	0.66319887213641	0.0
9	2.6930840255681397E-5	0.0	0.0	0.7804826814499026	0.0
10	2.6930840255681397E-5	0.0	0.0	0.924481884297031	0.0
11	2.6930840255681397E-5	0.0	0.0	1.041711831930012	0.0
12	5.3861680511362794E-5	0.0	0.0	1.1300719188089028	0.0
13	8.079252076704419E-5	0.0	0.0	1.1756658313617714	0.0
14	8.079252076704419E-5	0.0	0.0	1.2060976808506914	0.0
15	8.079252076704419E-5	0.0	0.0	1.232759212703816	0.0
16	8.079252076704419E-5	0.0	0.0	1.2715126918317414	0.0
17	1.3465420127840698E-4	0.0	0.0	1.323192974282394	0.0
18	1.3465420127840698E-4	0.0	0.0	1.4015347885861713	0.0
19	1.3465420127840698E-4	0.0	0.0	1.4389417257013128	0.0
20	1.6158504153408838E-4	0.0	0.0	1.4818156233883575	0.0
21	1.6158504153408838E-4	0.0	0.0	1.5317723320626466	0.0
22	1.6158504153408838E-4	0.0	0.0	1.588865713404691	0.0
23	1.8851588178976978E-4	0.0	0.0	1.6549001337116218	0.0
24	1.8851588178976978E-4	0.0	0.0	1.7089772609450302	0.0
25	1.8851588178976978E-4	0.0	0.0	1.7583953528142056	0.0
26	1.8851588178976978E-4	0.0	0.0	1.8077057213223582	0.0
27	1.8851588178976978E-4	0.0	0.0	1.862159880319346	0.0
28	2.1544672204545118E-4	0.0	0.0	1.9195225700639473	0.0
29	2.4237756230113258E-4	0.0	0.0	1.9833217306296564	0.0
30	2.4237756230113258E-4	0.0	0.0	2.0770410547194276	0.0
31	2.4237756230113258E-4	0.0	0.0	2.1484077813969833	0.0
32	2.6930840255681395E-4	0.0	0.0	2.2160311212789994	0.0
33	2.962392428124954E-4	0.0	0.0	2.2835467377999925	0.0
34	3.2317008306817675E-4	0.0	0.0	2.3578758569056735	0.0
35	3.2317008306817675E-4	0.0	0.0	2.453911233257433	0.0
36	3.501009233238582E-4	0.0	0.0	2.533545727893483	0.0
37	3.501009233238582E-4	0.0	0.0	2.6159271682356127	0.0
38	3.501009233238582E-4	0.0	0.0	2.7063609298141906	0.0
39	3.501009233238582E-4	0.0	0.0	2.8314546828018305	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	37275	0.0	43.484913	1
AGGGCGA	2790	0.0	39.919353	6
CGGTCTA	200	0.0	39.375	31
GGGCGAT	5235	0.0	39.19771	7
GCTACGA	870	0.0	38.793102	10
CTACGAA	865	0.0	38.75723	11
GCGATCG	300	0.0	38.250004	9
AGGGATC	4980	0.0	37.95181	6
ATACTAT	5750	0.0	37.643475	45
GTTTTTT	44215	0.0	37.55513	2
GCCCTAC	5875	0.0	37.531914	26
CCCTACA	5870	0.0	37.487225	27
ACGGGAT	1795	0.0	37.479107	5
AGGGATG	8335	0.0	37.468506	6
TAGGGAT	6730	0.0	37.343983	5
AAGGGAT	7105	0.0	37.336384	5
GCCCTAG	5975	0.0	37.20502	20
CGTTGAT	1055	0.0	37.10901	25
TAAGGGA	4955	0.0	37.053482	4
TACGGGA	1290	0.0	36.976746	4
>>END_MODULE