FastQCFastQC Report
Thu 26 May 2016
SRR1545237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545237_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1586616
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77770.4901627110781689No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34140.21517493835937618No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29620.18668663369082375No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT22110.13935318942957842No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19660.1239115198636595No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16720.10538151638455683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA207.0341E-445.021
CGTATTA207.0341E-445.019
ACGGGTA1350.043.3333365
ATATACG1000.042.751
CGAATAT3500.042.42857414
GGTACCT7950.041.6037758
CTATACG650.041.5384641
GCTACGA3750.040.810
CGTATGA501.0822987E-940.515
GGGTACC8750.039.8571437
CTACGAA3850.039.74025711
TTGATCC7550.039.6357617
CATATGC7350.039.48979633
TGAATCG403.4593359E-739.3751
TACGAAT3900.039.2307712
AGCTACG3850.039.155849
TATAGCG750.039.0000041
ATAGGGT5250.039.04
TAGGGTA6200.038.8306475
AACGGGA4200.038.571434
Produced by FastQC (version 0.11.5)