FastQCFastQC Report
Thu 26 May 2016
SRR1545236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545236_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3812529
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194380.5098453021603245No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79180.20768366614391653No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT53550.14045794799200217No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC49890.13085802101439753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA33200.040.5271076
ACGGGCT8900.040.449445
CAATTCG850.039.70588313
GGGCGAT59800.039.4690677
CGGTCTA3950.039.30379531
GCTACGA7350.039.18367410
AGTACGG5750.039.1304362
CGTTTTT136500.038.9835171
AAGGGCG16900.038.875745
AGGGTAC14800.038.766896
AACGGGA8900.038.6797754
CGTAAGG6600.038.5227282
GGGTACC19150.038.4203647
GTAGGGC14900.037.751684
ACGGGAC8350.037.7245525
CTACGAA7650.037.64705711
GCGCGAC19100.037.5785339
GGCGATT19550.037.519188
CGTATGG3500.037.2857132
ACCCGCT18300.037.1311534