##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545236_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3812529
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20574610711158	31.0	31.0	33.0	30.0	34.0
2	31.47681211080624	31.0	31.0	34.0	30.0	34.0
3	31.571200114149953	31.0	31.0	34.0	30.0	34.0
4	35.33796778988435	37.0	35.0	37.0	33.0	37.0
5	35.18918492160978	37.0	35.0	37.0	32.0	37.0
6	35.19477622334151	37.0	35.0	37.0	32.0	37.0
7	35.83650537477879	37.0	35.0	37.0	35.0	37.0
8	35.912431879206686	37.0	35.0	37.0	35.0	37.0
9	37.533330762861084	39.0	37.0	39.0	35.0	39.0
10	36.73988788019711	39.0	37.0	39.0	32.0	39.0
11	36.45137623871189	38.0	35.0	39.0	32.0	39.0
12	35.76897513435308	37.0	35.0	39.0	31.0	39.0
13	35.47973195744872	37.0	35.0	39.0	30.0	39.0
14	36.31862183867978	38.0	35.0	40.0	30.0	41.0
15	36.66920776209178	38.0	35.0	40.0	31.0	41.0
16	36.53477599776946	38.0	35.0	40.0	31.0	41.0
17	36.57446985977025	38.0	35.0	40.0	31.0	41.0
18	36.63812498213128	38.0	35.0	40.0	31.0	41.0
19	36.57861068073187	38.0	35.0	40.0	31.0	41.0
20	36.47029517677112	38.0	35.0	40.0	31.0	41.0
21	36.358884614385886	38.0	35.0	40.0	31.0	41.0
22	36.23650102071355	38.0	35.0	40.0	30.0	41.0
23	36.15185825471754	38.0	34.0	40.0	30.0	41.0
24	35.95661331362987	37.0	34.0	40.0	30.0	41.0
25	35.90101504801668	37.0	34.0	40.0	30.0	41.0
26	35.76413425314273	37.0	34.0	40.0	30.0	41.0
27	35.771160035766286	37.0	34.0	40.0	30.0	41.0
28	35.77705087620317	37.0	34.0	40.0	30.0	41.0
29	35.729147502878014	37.0	34.0	40.0	30.0	41.0
30	35.63345144391033	37.0	34.0	40.0	29.0	41.0
31	35.54659912095095	37.0	34.0	40.0	29.0	41.0
32	35.423397172847736	37.0	34.0	40.0	29.0	41.0
33	35.24388955467617	37.0	34.0	40.0	27.0	41.0
34	35.07582053802083	37.0	34.0	40.0	27.0	41.0
35	34.94235715977505	37.0	34.0	40.0	27.0	41.0
36	34.74281323499441	36.0	33.0	40.0	25.0	41.0
37	34.57801343937318	36.0	33.0	40.0	25.0	41.0
38	34.60381652178908	36.0	33.0	40.0	25.0	41.0
39	34.594550493910994	36.0	33.0	40.0	25.0	41.0
40	34.433851126116025	36.0	33.0	40.0	24.0	41.0
41	34.41070113827331	36.0	33.0	40.0	24.0	41.0
42	34.337957035867795	36.0	33.0	40.0	24.0	41.0
43	34.20844169316483	35.0	33.0	40.0	24.0	41.0
44	34.14668609733854	35.0	33.0	40.0	24.0	41.0
45	34.08622544248188	35.0	33.0	40.0	23.0	41.0
46	33.99729365993019	35.0	33.0	40.0	23.0	41.0
47	34.01433956305644	35.0	33.0	40.0	23.0	41.0
48	33.97891714397451	35.0	33.0	40.0	23.0	41.0
49	33.952425279912624	35.0	33.0	39.0	24.0	41.0
50	33.771413148595066	35.0	33.0	39.0	24.0	41.0
51	33.59424754539572	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	4.0
12	8.0
13	24.0
14	46.0
15	126.0
16	366.0
17	946.0
18	2100.0
19	3946.0
20	7097.0
21	11258.0
22	16920.0
23	24152.0
24	34419.0
25	47222.0
26	61361.0
27	73485.0
28	84079.0
29	97170.0
30	116580.0
31	141745.0
32	176217.0
33	226941.0
34	357946.0
35	496662.0
36	295122.0
37	359819.0
38	487430.0
39	688866.0
40	469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.03619251158483	23.981325781390776	26.712452547901933	14.27002915912246
2	32.69428245660558	25.412737844092465	28.826718432830283	13.06626126647168
3	29.808612603340194	26.145112601110704	30.865129157050347	13.181145638498752
4	26.830720500748978	28.09646824981528	29.532234377758176	15.540576871677567
5	22.720246849269866	32.75665575265133	29.342675163913505	15.1804222341653
6	21.58732431936911	41.48057627889519	26.547548884218326	10.384550517517376
7	86.68542586823602	4.826691154349253	6.66654601184673	1.8213369655679996
8	87.6059434564301	4.076139486414398	6.4945342055103055	1.823382851645194
9	82.77715395738629	5.879535604843924	8.296382794727593	3.0469276430421908
10	46.67167121876319	26.09268021305543	14.950050216011471	12.285598352169911
11	39.581521871702485	23.799005856742337	21.989419621463863	14.630052650091319
12	36.493650277807724	22.6608112357965	25.942622338085826	14.902916148309952
13	22.083058253458532	36.85705734959656	24.67131397557894	16.388570421365976
14	16.635335757446043	38.4272486845346	27.82885061333304	17.10856494468632
15	15.739919617660615	25.06651096949033	42.734206087350415	16.45936332549864
16	17.983758287477944	20.69969828426223	41.54326432664512	19.7732791016147
17	17.265311293369834	21.300428141005618	29.914316717328575	31.519943848295974
18	20.370022103438426	24.507800465255478	34.257680400594985	20.86449703071111
19	28.80284976192968	25.169539693993148	26.0867523892933	19.94085815478387
20	30.99934452957604	23.36517309114239	26.41383186855759	19.221650510723983
21	23.237777338874015	28.99267126886117	27.5450757227027	20.22447566956212
22	22.34789558322048	25.478730784736324	24.962721595035735	27.210652037007456
23	20.621692320242026	30.219704558312866	26.11670625980812	23.04189686163699
24	19.850471957065768	24.29940860777715	37.920524670107426	17.929594765049657
25	19.209375194260815	26.516834363751723	34.19024484797362	20.083545594013845
26	18.18226169558317	33.618052479076226	27.07134817859746	21.128337646743145
27	18.065488813330994	34.10667827051283	27.814424493557947	20.01340842259823
28	17.521125740945184	28.87839017093378	35.236453283371745	18.364030804749287
29	17.91545716767007	25.858688550303487	34.33754864553161	21.88830563649483
30	19.932412317388273	31.542081384823568	29.589335582758846	18.936170715029316
31	27.806345866483902	28.153097327259673	25.605339657744242	18.43521714851218
32	29.331999835279944	26.02409057085205	26.571312637884198	18.072596955983812
33	28.186303632051064	27.241497704017466	24.704703885531103	19.867494778400374
34	20.14536807457727	27.367345927073604	27.01094208070286	25.476343917646265
35	21.191891261679583	26.242397107012174	30.149541157588573	22.416170473719678
36	27.91084343227291	28.477894856668627	24.870892785340125	18.740368925718336
37	20.999184530793077	34.088134149274666	27.006220805140106	17.90646051479215
38	20.605272773007105	34.16029097745879	24.982288659312495	20.252147590221607
39	21.489226704898506	32.99909325279886	25.29955837712972	20.21212166517291
40	24.594435871832058	26.20635803688313	27.606085094696986	21.593120996587828
41	18.174760113300124	25.858163964129847	28.68334903157458	27.283726890995453
42	20.284540786443852	27.170888405045574	25.268529104958937	27.276041703551634
43	20.537207716977367	26.602682891067847	26.884595500781767	25.975513891173023
44	19.34122992900513	29.578476648964507	29.752245818982626	21.32804760304774
45	18.409827177708024	36.309861511873095	24.53284945504677	20.747461855372116
46	20.68207218882794	33.907178148677694	25.58383162462502	19.82691803786935
47	20.32076346173367	29.1621912908728	27.178967032119623	23.338078215273903
48	20.304265226572703	27.245589476171855	31.496285011864828	20.953860285390615
49	21.1163246233668	28.00031160418714	30.99040033531548	19.892963437130575
50	19.478960028894207	33.88427996219832	26.9036117495762	19.733148259331273
51	18.710887182759787	32.412684598595845	26.802287930137712	22.07414028850666
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8962.0
1	8945.5
2	8929.0
3	21385.5
4	33842.0
5	24411.5
6	14981.0
7	14935.5
8	14890.0
9	15584.0
10	16278.0
11	16973.0
12	17668.0
13	17960.0
14	18252.0
15	17645.5
16	17039.0
17	16360.5
18	15682.0
19	15534.5
20	15387.0
21	16574.0
22	17761.0
23	19846.0
24	21931.0
25	23836.0
26	34260.0
27	42779.0
28	49156.0
29	55533.0
30	67951.0
31	80369.0
32	93655.5
33	106942.0
34	119849.0
35	132756.0
36	138096.5
37	143437.0
38	153058.5
39	162680.0
40	214375.5
41	266071.0
42	301507.0
43	336943.0
44	342367.5
45	347792.0
46	329580.5
47	311369.0
48	292519.0
49	273669.0
50	252698.5
51	231728.0
52	208076.0
53	184424.0
54	167655.5
55	150887.0
56	145222.0
57	139557.0
58	136089.0
59	132621.0
60	127481.0
61	122341.0
62	110702.5
63	99064.0
64	86601.5
65	74139.0
66	62367.0
67	50595.0
68	44708.0
69	38821.0
70	33824.5
71	28828.0
72	25758.0
73	22688.0
74	17911.5
75	10427.0
76	7719.0
77	6036.5
78	4354.0
79	3203.0
80	2052.0
81	1566.5
82	1081.0
83	818.5
84	556.0
85	333.0
86	110.0
87	96.0
88	82.0
89	54.0
90	26.0
91	26.0
92	26.0
93	14.0
94	2.0
95	3.5
96	5.0
97	5.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3812529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.35012033626535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76990862755092	12.878974849370836
2	7.2636050768780684	2.3752163416412864
3	2.459197917594381	1.2062454565008385
4	1.436522215124185	0.9394924433199552
5	0.8887787590657507	0.7265819831520806
6	0.6907599454552777	0.6776404938999528
7	0.5063217135371788	0.579489466163786
8	0.4256529961319806	0.5567582166599817
9	0.34723201808111864	0.5109556752207499
>10	4.268397932622311	16.942962579546837
>50	1.6326738921901067	19.016533115543492
>100	1.286736942150812	39.38182622477043
>500	0.019071067233320044	1.9966686199191435
>1k	0.0046433902828953155	1.3237192523588397
>5k	3.316707344925225E-4	0.3598878663776911
>10k+	1.6583536724626126E-4	0.5270474155540992
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19438	0.5098453021603245	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7918	0.20768366614391653	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5355	0.14045794799200217	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4989	0.13085802101439753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.622930868197986E-5	0.0	0.0	0.01568512659182396	0.0
2	2.622930868197986E-5	0.0	0.0	0.05201271911636607	0.0
3	2.622930868197986E-5	0.0	0.0	0.0802354552581764	0.0
4	2.622930868197986E-5	0.0	0.0	0.1111073515768667	0.0
5	2.622930868197986E-5	0.0	0.0	0.20012962524350633	0.0
6	2.622930868197986E-5	0.0	0.0	0.3153025196660799	0.0
7	2.622930868197986E-5	0.0	0.0	0.3892167115318992	0.0
8	2.622930868197986E-5	0.0	0.0	0.5839693284955997	0.0
9	2.622930868197986E-5	0.0	0.0	0.6762178071301228	0.0
10	2.622930868197986E-5	0.0	0.0	0.8264330579518214	0.0
11	2.622930868197986E-5	0.0	0.0	0.9545370015546111	0.0
12	2.622930868197986E-5	0.0	0.0	1.065303372118612	0.0
13	2.622930868197986E-5	0.0	0.0	1.1141161155757766	0.0
14	5.245861736395972E-5	0.0	0.0	1.1343126832609012	0.0
15	5.245861736395972E-5	0.0	0.0	1.1620632918464358	0.0
16	5.245861736395972E-5	0.0	0.0	1.2207644846767067	0.0
17	5.245861736395972E-5	0.0	0.0	1.2925541025392857	0.0
18	5.245861736395972E-5	0.0	0.0	1.4021401542125975	0.0
19	5.245861736395972E-5	0.0	0.0	1.4510840442131718	0.0
20	1.0491723472791945E-4	0.0	0.0	1.5066901786189693	0.0
21	1.0491723472791945E-4	0.0	0.0	1.577404394825587	0.0
22	1.0491723472791945E-4	0.0	0.0	1.6564857605017562	0.0
23	1.0491723472791945E-4	0.0	0.0	1.7502817683485161	0.0
24	1.311465434098993E-4	0.0	0.0	1.8232779344104662	0.0
25	1.8360516077385903E-4	0.0	0.0	1.8847856632697089	0.0
26	1.8360516077385903E-4	0.0	0.0	1.9440639008909835	0.0
27	1.8360516077385903E-4	0.0	0.0	2.0052568780460422	0.0
28	1.8360516077385903E-4	0.0	0.0	2.072141615185091	0.0
29	2.098344694558389E-4	0.0	0.0	2.150645936070257	0.0
30	2.098344694558389E-4	0.0	0.0	2.2578451206535086	0.0
31	2.098344694558389E-4	0.0	0.0	2.3465788719246463	0.0
32	2.098344694558389E-4	0.0	0.0	2.434263450848505	0.0
33	2.098344694558389E-4	0.0	0.0	2.522997202119643	0.0
34	2.098344694558389E-4	0.0	0.0	2.6144325721850246	0.0
35	2.098344694558389E-4	0.0	0.0	2.7369759023472344	0.0
36	2.098344694558389E-4	0.0	0.0	2.836909568425578	0.0
37	2.098344694558389E-4	0.0	0.0	2.936948151738649	0.0
38	2.098344694558389E-4	0.0	0.0	3.0420489916273423	0.0
39	2.098344694558389E-4	0.0	0.0	3.1528415915000254	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGCGA	3320	0.0	40.527107	6
ACGGGCT	890	0.0	40.44944	5
CAATTCG	85	0.0	39.705883	13
GGGCGAT	5980	0.0	39.469067	7
CGGTCTA	395	0.0	39.303795	31
GCTACGA	735	0.0	39.183674	10
AGTACGG	575	0.0	39.130436	2
CGTTTTT	13650	0.0	38.983517	1
AAGGGCG	1690	0.0	38.87574	5
AGGGTAC	1480	0.0	38.76689	6
AACGGGA	890	0.0	38.679775	4
CGTAAGG	660	0.0	38.522728	2
GGGTACC	1915	0.0	38.420364	7
GTAGGGC	1490	0.0	37.75168	4
ACGGGAC	835	0.0	37.724552	5
CTACGAA	765	0.0	37.647057	11
GCGCGAC	1910	0.0	37.578533	9
GGCGATT	1955	0.0	37.51918	8
CGTATGG	350	0.0	37.285713	2
ACCCGCT	1830	0.0	37.13115	34
>>END_MODULE