FastQCFastQC Report
Thu 26 May 2016
SRR1545235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545235_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1610387
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74440.46224913638771303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26080.16194864961031108No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20600.12791956219219355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT1800.042.528
ACGGGTA1250.041.45
AGTACGG1500.040.52
TCGATGG950.040.2631572
CGGTCTA1850.040.13513631
TAATACG451.9292202E-840.01
AGGGCGA16300.039.75466
CGCTAAT403.4593722E-739.37500414
ACGTAAG403.4593722E-739.3750041
CAATCGA800.039.37500441
GGGCGAT31550.038.866887
CGGGTAT1750.038.5714266
TATGGGC5450.038.3944974
CGTTTTT56500.038.3893781
GGCGATA5700.038.2894748
ACGGTCT2000.038.25000430
ACGGGAT4700.037.819155
TAGGGCG7400.037.70275
CGTTGAT2450.037.6530625
CGCATAT601.5643309E-1037.50000436
Produced by FastQC (version 0.11.5)