##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545235_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1610387 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.26257415143068 31.0 31.0 33.0 30.0 34.0 2 31.522193112587225 31.0 31.0 34.0 30.0 34.0 3 31.610412279781194 31.0 31.0 34.0 30.0 34.0 4 35.386849869006646 37.0 35.0 37.0 33.0 37.0 5 35.25310810382846 37.0 35.0 37.0 33.0 37.0 6 35.25359245945229 37.0 35.0 37.0 32.0 37.0 7 35.872652970993926 37.0 35.0 37.0 35.0 37.0 8 35.95101115446163 37.0 35.0 37.0 35.0 37.0 9 37.58564369930954 39.0 37.0 39.0 35.0 39.0 10 36.80191717891414 39.0 37.0 39.0 32.0 39.0 11 36.51655968410078 39.0 35.0 39.0 32.0 39.0 12 35.89364668244341 37.0 35.0 39.0 31.0 39.0 13 35.6016802172397 37.0 35.0 39.0 30.0 39.0 14 36.41677435299714 38.0 35.0 40.0 30.0 41.0 15 36.75174414597237 38.0 35.0 41.0 31.0 41.0 16 36.621554943004384 38.0 35.0 40.0 31.0 41.0 17 36.676298926903904 38.0 35.0 40.0 31.0 41.0 18 36.72584229753469 38.0 35.0 40.0 31.0 41.0 19 36.68533588510091 38.0 35.0 40.0 31.0 41.0 20 36.557696379814296 38.0 35.0 40.0 31.0 41.0 21 36.46810921846736 38.0 35.0 40.0 31.0 41.0 22 36.35692538501615 38.0 35.0 40.0 31.0 41.0 23 36.242170981261026 38.0 35.0 40.0 30.0 41.0 24 36.07210130235776 38.0 34.0 40.0 30.0 41.0 25 36.045317057328454 37.0 34.0 40.0 30.0 41.0 26 35.92941634526359 37.0 34.0 40.0 30.0 41.0 27 35.901933510392226 37.0 34.0 40.0 30.0 41.0 28 35.90263396314054 37.0 34.0 40.0 30.0 41.0 29 35.8742643848963 37.0 34.0 40.0 30.0 41.0 30 35.78668543648204 37.0 34.0 40.0 30.0 41.0 31 35.680768038986905 37.0 34.0 40.0 29.0 41.0 32 35.553203049950106 37.0 34.0 40.0 29.0 41.0 33 35.36518302743378 37.0 34.0 40.0 28.0 41.0 34 35.18799828861013 37.0 34.0 40.0 27.0 41.0 35 35.0179112225819 37.0 34.0 40.0 27.0 41.0 36 34.8523448090428 37.0 33.0 40.0 25.0 41.0 37 34.67179504057099 37.0 33.0 40.0 25.0 41.0 38 34.70543540155255 37.0 33.0 40.0 25.0 41.0 39 34.666806798614246 37.0 33.0 40.0 25.0 41.0 40 34.54488765743886 36.0 33.0 40.0 24.0 41.0 41 34.521542958307535 36.0 33.0 40.0 24.0 41.0 42 34.44965775307426 36.0 33.0 40.0 24.0 41.0 43 34.3265444889955 36.0 33.0 40.0 24.0 41.0 44 34.27623235905406 36.0 33.0 40.0 24.0 41.0 45 34.22901203251144 36.0 33.0 40.0 24.0 41.0 46 34.1355227035489 35.0 33.0 40.0 23.0 41.0 47 34.13608157542255 35.0 33.0 40.0 24.0 41.0 48 34.112954215353206 35.0 33.0 40.0 24.0 41.0 49 34.09288823121399 36.0 33.0 40.0 24.0 41.0 50 33.91488008782982 35.0 33.0 39.0 24.0 41.0 51 33.738041849567836 35.0 33.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 2.0 12 3.0 13 8.0 14 10.0 15 55.0 16 174.0 17 353.0 18 781.0 19 1584.0 20 2828.0 21 4590.0 22 7011.0 23 10402.0 24 14460.0 25 19687.0 26 25177.0 27 29934.0 28 33306.0 29 38341.0 30 46496.0 31 57551.0 32 72805.0 33 93935.0 34 149561.0 35 203800.0 36 126087.0 37 156072.0 38 214346.0 39 300885.0 40 142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.80841127008601 24.044034135894044 25.949663031308624 14.197891562711323 2 33.20760786071919 25.16289562695178 28.277923256956246 13.351573255372776 3 29.392437966774448 26.49816472686379 30.550730973362306 13.558666332999458 4 26.359005630323644 27.656147249077396 30.866990356976302 15.117856763622658 5 22.80687809824595 32.046272107263654 29.38163311055045 15.765216683939947 6 21.53805265442406 40.976796260774584 27.421731546516458 10.063419538284897 7 86.28981729236513 4.494447607935236 7.356927247922394 1.8588078517772437 8 87.34428432420282 4.027354915309178 6.839039311668562 1.789321448819445 9 82.70434373849267 5.28593437478072 8.34998047053286 3.6597414161937474 10 45.12319088517232 28.106225397994393 15.110343041765736 11.660240675067545 11 37.9986301429408 24.045276073391054 23.76714417093531 14.18894961273284 12 34.36267182981482 22.926476679208164 27.442658193341103 15.268193297635909 13 22.03513813760295 35.03008904070885 27.97824373892735 14.956529082760852 14 17.462075886106877 37.50738176599786 29.333197548166996 15.697344799728263 15 16.368301532488776 24.88917260261043 42.77592901582042 15.966596849080375 16 19.110996300889166 21.44571460152125 40.62892956786164 18.81435952972795 17 19.12782455397367 21.941309759703724 29.466084860347237 29.464780825975375 18 21.332760386168044 24.450830763040187 34.775740241320875 19.44066860947089 19 28.221787682091325 25.324906373436946 26.66762709833102 19.785678846140712 20 29.647966606784582 24.350854794530754 27.313310403027348 18.687868195657316 21 23.48329935599331 28.4332896378324 29.5211647883397 18.562246217834595 22 22.137287496732153 24.2433651041644 27.425022680883536 26.194324718219907 23 19.821881324178598 30.296941045847987 27.579022930512977 22.30215469946044 24 19.62509632777711 25.368995154580855 37.37685413506194 17.629054382580087 25 19.409620172045603 26.66868274520348 34.57423588243074 19.347461200320172 26 18.26598202792248 32.57800764660917 28.050834985627677 21.10517533984067 27 18.49213884612829 33.524488213081696 29.395356519892424 18.588016420897585 28 17.222257755433944 28.789663602599873 35.71358934218918 18.27448929977701 29 17.820623241494125 26.09285842471406 35.33486050247549 20.751657831316322 30 20.052881698622755 31.412821886912894 30.916543663107067 17.617752751357283 31 27.269842590631942 27.288595846836817 27.269283718758285 18.172277843772957 32 28.447013047174373 25.426869441941598 28.366597594242876 17.759519916641157 33 27.626837524147923 26.88670487280387 25.815844266005623 19.670613337042585 34 20.115289057847583 27.230783656350926 28.287548272558087 24.3663790132434 35 20.654041544051214 26.041876890461737 31.40996543066977 21.89411613481728 36 28.49135021581769 26.750402232506843 26.26890306491545 18.489344486760015 37 20.690181925214247 33.079067329778496 27.7638232300683 18.466927514938956 38 20.36653301349303 33.19140057638319 25.50653973237489 20.93552667774889 39 20.48731143507741 32.34303307217458 26.856153210377382 20.313502282370635 40 24.1305350825609 26.215934430667907 27.56356080867518 22.08996967809601 41 18.57677688654963 25.860802403397443 28.24190706954291 27.320513640510015 42 19.828898271036714 27.085166484826317 27.02729219746558 26.058643046671392 43 20.07623012356657 26.679922279551437 28.431861409710834 24.811986187171158 44 19.22829729748191 28.70055458718929 31.260249865405026 20.810898249923778 45 18.981710607450257 34.79679108189522 26.00089295306035 20.220605357594167 46 21.346483795510025 32.64333355895198 26.503442961226092 19.506739684311906 47 20.169002854593337 28.512214765767485 28.643549656076463 22.675232723562722 48 20.76612640315651 27.38012664036657 31.69797073622676 20.155776220250164 49 20.791151443721294 27.228672362606005 31.863769392077806 20.116406801594895 50 20.134725379675817 33.1494230889842 27.943283198386474 18.772568332953508 51 18.905020967009794 32.26379745986524 27.34895400919158 21.48222756393339 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3070.0 1 3158.0 2 3246.0 3 8244.0 4 13242.0 5 9712.0 6 6182.0 7 6180.5 8 6179.0 9 6478.5 10 6778.0 11 7160.5 12 7543.0 13 7400.0 14 7257.0 15 7280.5 16 7304.0 17 7362.0 18 7420.0 19 7398.0 20 7376.0 21 7992.0 22 8608.0 23 9283.5 24 9959.0 25 11553.5 26 15798.0 27 18448.0 28 21860.0 29 25272.0 30 29609.0 31 33946.0 32 40178.5 33 46411.0 34 51523.0 35 56635.0 36 60716.5 37 64798.0 38 72773.0 39 80748.0 40 98789.5 41 116831.0 42 130987.0 43 145143.0 44 147186.0 45 149229.0 46 143325.0 47 137421.0 48 127576.5 49 117732.0 50 105924.0 51 94116.0 52 86458.5 53 78801.0 54 71638.5 55 64476.0 56 60630.0 57 56784.0 58 53734.5 59 50685.0 60 45740.5 61 40796.0 62 38274.5 63 35753.0 64 32389.5 65 29026.0 66 24344.5 67 19663.0 68 16938.5 69 14214.0 70 12068.5 71 9923.0 72 8600.5 73 7278.0 74 5902.0 75 3340.0 76 2154.0 77 1729.0 78 1304.0 79 883.5 80 463.0 81 349.5 82 236.0 83 209.5 84 183.0 85 121.0 86 59.0 87 37.0 88 15.0 89 9.0 90 3.0 91 2.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1610387.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.982707794388624 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3284385003516 16.0157732145484 2 6.779411060590698 2.8450080260484176 3 2.8666066416531972 1.8044750856978824 4 1.5650483814499934 1.313558114881843 5 1.0943878615562344 1.1481610356380147 6 0.8340689653528857 1.050061522822059 7 0.6627780737715568 0.9734815058133426 8 0.564532459431414 0.9476335709357528 9 0.4725741581521915 0.8924296924517967 >10 6.864219846279142 34.06064314119457 >50 1.35627200733045 20.249302951285088 >100 0.6064784674363232 17.25461878534701 >500 0.003354079005187068 0.43365434304167066 >1k 0.0015245813659941218 0.5349341932976407 >5k 3.0491627319882437E-4 0.4762648169964881 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7444 0.46224913638771303 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2608 0.16194864961031108 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 2060 0.12791956219219355 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.01757341558271397 0.0 2 0.0 0.0 0.0 0.062034777975728816 0.0 3 0.0 0.0 0.0 0.09258644040221388 0.0 4 0.0 0.0 0.0 0.14027684028745885 0.0 5 0.0 0.0 0.0 0.28589401181206753 0.0 6 0.0 0.0 0.0 0.42300391148214683 0.0 7 0.0 0.0 0.0 0.516149223758016 0.0 8 0.0 0.0 0.0 0.7277753732487905 0.0 9 0.0 0.0 0.0 0.8303594105019476 0.0 10 0.0 0.0 0.0 1.0383839412513887 0.0 11 0.0 0.0 0.0 1.1899003158868025 0.0 12 0.0 0.0 0.0 1.3392432999024457 0.0 13 0.0 0.0 0.0 1.3967450060140822 0.0 14 0.0 0.0 0.0 1.4213353684549117 0.0 15 0.0 0.0 0.0 1.4592144621137653 0.0 16 0.0 0.0 0.0 1.5329855494362534 0.0 17 0.0 0.0 0.0 1.6134630992426044 0.0 18 0.0 0.0 0.0 1.7376568489437632 0.0 19 0.0 0.0 0.0 1.8072674456512627 0.0 20 0.0 0.0 0.0 1.8780578829809231 0.0 21 0.0 0.0 0.0 1.9689056108873209 0.0 22 0.0 0.0 0.0 2.064596895032064 0.0 23 0.0 0.0 0.0 2.174694654142141 0.0 24 0.0 0.0 0.0 2.2644246383012283 0.0 25 0.0 0.0 0.0 2.33322797563567 0.0 26 0.0 0.0 0.0 2.4019692160952615 0.0 27 0.0 0.0 0.0 2.486234675267498 0.0 28 0.0 0.0 0.0 2.571990459436148 0.0 29 0.0 0.0 0.0 2.6797285373019033 0.0 30 0.0 0.0 0.0 2.8073997119946945 0.0 31 0.0 0.0 0.0 2.914641014861645 0.0 32 0.0 0.0 0.0 3.0180323114878598 0.0 33 0.0 0.0 0.0 3.128254264347638 0.0 34 0.0 0.0 0.0 3.2379173453337615 0.0 35 0.0 0.0 0.0 3.381174835614048 0.0 36 0.0 0.0 0.0 3.498351638457091 0.0 37 0.0 0.0 0.0 3.620061513164227 0.0 38 0.0 0.0 0.0 3.76828675343256 0.0 39 0.0 0.0 0.0 4.00841536847975 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGGT 180 0.0 42.5 28 ACGGGTA 125 0.0 41.4 5 AGTACGG 150 0.0 40.5 2 TCGATGG 95 0.0 40.263157 2 CGGTCTA 185 0.0 40.135136 31 TAATACG 45 1.9292202E-8 40.0 1 AGGGCGA 1630 0.0 39.7546 6 CGCTAAT 40 3.4593722E-7 39.375004 14 ACGTAAG 40 3.4593722E-7 39.375004 1 CAATCGA 80 0.0 39.375004 41 GGGCGAT 3155 0.0 38.86688 7 CGGGTAT 175 0.0 38.571426 6 TATGGGC 545 0.0 38.394497 4 CGTTTTT 5650 0.0 38.389378 1 GGCGATA 570 0.0 38.289474 8 ACGGTCT 200 0.0 38.250004 30 ACGGGAT 470 0.0 37.81915 5 TAGGGCG 740 0.0 37.7027 5 CGTTGAT 245 0.0 37.65306 25 CGCATAT 60 1.5643309E-10 37.500004 36 >>END_MODULE