FastQCFastQC Report
Thu 26 May 2016
SRR1545234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545234_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1543900
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56400.3653086339788847No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35270.22844743830558972No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34920.22618045210182008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTC302.165989E-645.00000444
GCCTATA302.165989E-645.00000417
AATCGGT302.165989E-645.00000426
AAATGCG302.165989E-645.0000041
TATCCGC302.165989E-645.00000432
CTATATG253.8914302E-545.01
CCGGATA453.8562575E-1045.020
ACGGGTA253.8914302E-545.05
TCGATAG207.0340396E-445.01
CGTTATT207.0340396E-445.045
CTCGGTT207.0340396E-445.033
ACTCGCG207.0340396E-445.025
GTATCAC207.0340396E-445.035
CGGCTAA207.0340396E-445.038
CACGTAC207.0340396E-445.042
ATACGCC406.8175723E-945.02
TATACGA207.0340396E-445.037
CGCCGAT453.8562575E-1045.038
TCGCGAT207.0340396E-445.037
AATCGAC253.8914302E-545.037