##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545234_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1543900
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.202694475030768	31.0	31.0	33.0	30.0	34.0
2	31.44555282077855	31.0	31.0	34.0	30.0	34.0
3	31.529870457931214	31.0	31.0	34.0	30.0	34.0
4	35.27608977265367	37.0	35.0	37.0	33.0	37.0
5	35.12465120797979	37.0	35.0	37.0	32.0	37.0
6	35.11500550553792	37.0	35.0	37.0	32.0	37.0
7	35.801882246259474	37.0	35.0	37.0	35.0	37.0
8	35.92024807306173	37.0	35.0	37.0	35.0	37.0
9	37.594986074227606	39.0	37.0	39.0	35.0	39.0
10	36.817699332858346	39.0	37.0	39.0	32.0	39.0
11	36.405465379882116	38.0	35.0	39.0	32.0	39.0
12	35.10539866571669	35.0	35.0	39.0	30.0	39.0
13	34.47517779648941	35.0	33.0	39.0	26.0	39.0
14	35.15421270807695	36.0	33.0	40.0	25.0	41.0
15	35.748683852581124	36.0	34.0	40.0	30.0	41.0
16	35.78599132068139	36.0	34.0	40.0	31.0	41.0
17	35.861132197681194	36.0	34.0	40.0	31.0	41.0
18	35.9571222229419	36.0	35.0	40.0	31.0	41.0
19	35.862244964052074	36.0	34.0	40.0	30.0	41.0
20	35.64211477427295	36.0	34.0	40.0	30.0	41.0
21	35.48602564932962	35.0	34.0	40.0	30.0	41.0
22	35.36817345683011	35.0	34.0	40.0	30.0	41.0
23	35.306190815467325	35.0	34.0	40.0	29.0	41.0
24	35.19426258177343	35.0	34.0	40.0	29.0	41.0
25	35.21650430727379	35.0	34.0	40.0	29.0	41.0
26	35.05227411101755	35.0	34.0	40.0	29.0	41.0
27	34.96629315370166	35.0	34.0	40.0	29.0	41.0
28	35.06499125591036	36.0	34.0	40.0	29.0	41.0
29	35.11880950838785	36.0	34.0	40.0	29.0	41.0
30	35.05378651467064	36.0	34.0	40.0	29.0	41.0
31	34.87321199559557	35.0	34.0	40.0	29.0	41.0
32	34.6590284344841	35.0	34.0	40.0	27.0	41.0
33	34.42746550942419	35.0	33.0	40.0	27.0	41.0
34	34.25072867413692	35.0	33.0	40.0	26.0	41.0
35	34.08910033033227	35.0	33.0	40.0	25.0	41.0
36	33.81795841699592	35.0	33.0	39.0	23.0	41.0
37	33.64451065483516	35.0	33.0	39.0	23.0	41.0
38	33.74032191204093	35.0	33.0	39.0	23.0	41.0
39	33.751822656907834	35.0	33.0	39.0	23.0	41.0
40	33.594031349180646	35.0	33.0	39.0	23.0	41.0
41	33.65889630157394	35.0	33.0	39.0	23.0	41.0
42	33.60812746939568	35.0	33.0	39.0	23.0	41.0
43	33.450417773171836	35.0	33.0	39.0	23.0	41.0
44	33.45738130707947	35.0	33.0	39.0	23.0	41.0
45	33.38200012954207	35.0	32.0	39.0	23.0	41.0
46	33.34482414664162	35.0	32.0	39.0	23.0	41.0
47	33.375202409482476	35.0	32.0	39.0	23.0	41.0
48	33.41350281754	35.0	33.0	38.0	23.0	41.0
49	33.4363805945981	35.0	32.0	38.0	24.0	40.0
50	33.13315240624393	35.0	32.0	38.0	23.0	40.0
51	32.97552820778548	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	1.0
13	5.0
14	23.0
15	50.0
16	138.0
17	455.0
18	953.0
19	1937.0
20	3586.0
21	5865.0
22	9086.0
23	12870.0
24	17690.0
25	23381.0
26	29319.0
27	33734.0
28	37793.0
29	43372.0
30	51232.0
31	63126.0
32	79603.0
33	104478.0
34	180147.0
35	268771.0
36	95924.0
37	111706.0
38	152127.0
39	216368.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.86566487466805	23.742081741045403	28.745579376902647	13.646674007383897
2	30.138739555670703	26.766889047218083	29.949089966966774	13.14528143014444
3	28.61856337845715	26.505214068268668	31.28473346719347	13.591489086080704
4	25.270937236867674	27.287194766500424	32.716626724528794	14.725241272103116
5	23.692790983872012	32.132845391540904	29.429043331822008	14.745320292765077
6	20.025260703413434	42.36569726018525	27.70185892868709	9.90718310771423
7	85.5729645702442	5.227670185892869	7.452490446272426	1.7468747975905177
8	88.05453721095927	4.22579182589546	6.107908543299437	1.611762419845845
9	84.69926808731135	4.460975451777965	7.349439730552497	3.4903167303581837
10	60.242178897597	19.263553338946824	11.677958416995919	8.816309346460264
11	55.328000518168274	17.848694863656974	16.397953235313167	10.425351382861585
12	47.5413563054602	21.289008355463437	19.879784960165814	11.289850378910552
13	20.57205777576268	48.01956085238681	20.49517455793769	10.913206813912819
14	12.71150981281171	50.078243409547255	26.139646350152212	11.070600427488827
15	11.767212902390051	21.445689487661117	55.26640326446013	11.520694345488698
16	13.039445559945593	15.96495887039316	52.35779519398924	18.637800375672
17	13.45158365179092	16.545825506833346	29.266403264460134	40.736187576915604
18	19.30591359544012	21.157652697713583	39.47308763520954	20.063346071636765
19	33.27313945203705	20.9606192110888	25.733985361746225	20.03225597512792
20	36.09262257918259	21.2667271196321	24.69764881145152	17.94300148973379
21	20.945333246971952	31.5383768378781	28.217954530733856	19.29833538441609
22	21.16296392253384	24.369648293283245	23.58682557160438	30.880562212578532
23	17.3445171319386	33.23259278450677	24.757238163093465	24.66565192046117
24	16.14495757497247	22.140812228771292	46.092687350216984	15.621542846039253
25	14.071377679901548	24.831336226439536	42.69421594662867	18.40307014703025
26	13.994624004145345	38.50663903102532	27.881663320163224	19.617073644666107
27	15.65710214392124	40.7224561176242	27.648876222553277	15.97156551590129
28	12.58948118401451	30.88529049808925	41.66584623356435	14.859382084331887
29	13.508452619988343	24.622320098451972	40.44407021180128	21.425157069758406
30	17.480277220027205	35.9685212772848	29.927974609754514	16.62322689293348
31	32.350346525034006	27.494267763456183	24.173197745968004	15.982187965541812
32	36.71798691625106	22.891314204287845	26.171708012176953	14.21899086728415
33	33.643953623939375	25.51732625170024	22.944232139387267	17.89448798497312
34	19.8887881339465	25.528920266856662	26.806593691301252	27.77569790789559
35	20.704449770062826	23.79260314787227	33.81585594921951	21.68709113284539
36	35.929529114579964	25.17391022734633	23.460392512468424	15.436168145605286
37	20.073191268864562	37.53546214133039	26.994559233110955	15.396787356694086
38	19.0108167627437	38.43053306561306	21.975646091068075	20.58300408057517
39	21.4379817345683	36.62309735086469	24.601139970205324	17.33778094436168
40	27.558326316471277	23.685342314916767	23.69181941835611	25.064511950255845
41	15.638124230843967	23.077530928168922	26.498024483450997	34.78632035753611
42	20.151434678411814	22.892285769803745	24.944037826284085	32.01224172550036
43	19.61551913984066	23.642852516354687	27.174298853552692	29.567329490251957
44	16.895200466351447	29.91404883735993	32.277867737547766	20.91288295874085
45	15.25176501068722	43.100654187447375	22.1102403005376	19.537340501327808
46	20.456765334542393	37.52179545307339	24.156551590128895	17.864887622255328
47	19.87175335190103	28.111989118466223	25.472439924865597	26.54381760476715
48	20.945462789040743	24.193147224561177	35.28000518168275	19.58138480471533
49	21.680484487337264	23.905628602888786	36.17999870457931	18.23388820519464
50	19.01910745514606	38.91009780426194	24.503141395168083	17.567653345423924
51	17.249174169311484	37.26782822721679	24.222682816244575	21.260314787227152
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3928.0
1	3612.0
2	3296.0
3	6826.0
4	10356.0
5	7681.0
6	5006.0
7	5295.0
8	5584.0
9	6127.0
10	6670.0
11	7173.5
12	7677.0
13	7518.5
14	7360.0
15	7284.5
16	7209.0
17	6480.0
18	5751.0
19	5626.0
20	5501.0
21	5917.0
22	6333.0
23	5652.5
24	4972.0
25	7129.0
26	9829.5
27	10373.0
28	12766.5
29	15160.0
30	16460.0
31	17760.0
32	23751.0
33	29742.0
34	33899.0
35	38056.0
36	43288.5
37	48521.0
38	62648.5
39	76776.0
40	107060.0
41	137344.0
42	162858.5
43	188373.0
44	187759.5
45	187146.0
46	168242.5
47	149339.0
48	135560.0
49	121781.0
50	110216.0
51	98651.0
52	89292.0
53	79933.0
54	69553.0
55	59173.0
56	52919.5
57	46666.0
58	43970.0
59	41274.0
60	37106.0
61	32938.0
62	28460.5
63	23983.0
64	21178.5
65	18374.0
66	15176.5
67	11979.0
68	10119.5
69	8260.0
70	7190.0
71	6120.0
72	4878.0
73	3636.0
74	2722.0
75	1381.0
76	954.0
77	671.5
78	389.0
79	371.5
80	354.0
81	212.0
82	70.0
83	39.0
84	8.0
85	8.5
86	9.0
87	10.0
88	11.0
89	7.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	2.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1543900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.468902694097295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.75124739771337	13.931667805235351
2	7.549650502878675	2.637682200184606
3	2.6291952860057033	1.3778746984503882
4	1.2939182530524416	0.9041332822675786
5	0.7885935304605362	0.6887931824404879
6	0.5472389416159934	0.5735798292906349
7	0.3863146773703722	0.47239454557992355
8	0.3148217391447829	0.4399672261685355
9	0.2283633736618141	0.3590331798054611
>10	3.796760818967683	16.70100157290241
>50	1.4144503837166997	17.183094969422232
>100	1.2830157690071726	42.10844412838149
>500	0.014518939613541561	1.6085477984903722
>1k	0.0015283094330043748	0.6373453497006043
>5k	3.820773582510937E-4	0.37644023167990454
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5640	0.3653086339788847	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3527	0.22844743830558972	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3492	0.22618045210182008	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03083101237126757	0.0
2	0.0	0.0	0.0	0.0978690329684565	0.0
3	0.0	0.0	0.0	0.13232722326575555	0.0
4	6.477103439341926E-5	0.0	0.0	0.19178703283891443	0.0
5	6.477103439341926E-5	0.0	0.0	0.37852192499514214	0.0
6	6.477103439341926E-5	0.0	0.0	0.5048254420623097	0.0
7	6.477103439341926E-5	0.0	0.0	0.6066455081287648	0.0
8	6.477103439341926E-5	0.0	0.0	0.8450676857309412	0.0
9	6.477103439341926E-5	0.0	0.0	0.9425480924930371	0.0
10	6.477103439341926E-5	0.0	0.0	1.2002720383444523	0.0
11	6.477103439341926E-5	0.0	0.0	1.3627825636375412	0.0
12	6.477103439341926E-5	0.0	0.0	1.5684305978366475	0.0
13	6.477103439341926E-5	0.0	0.0	1.6341084267115746	0.0
14	6.477103439341926E-5	0.0	0.0	1.655936265302157	0.0
15	6.477103439341926E-5	0.0	0.0	1.7028952652373859	0.0
16	6.477103439341926E-5	0.0	0.0	1.7933804002849925	0.0
17	6.477103439341926E-5	0.0	0.0	1.8957834056609884	0.0
18	6.477103439341926E-5	0.0	0.0	2.0669732495627957	0.0
19	6.477103439341926E-5	0.0	0.0	2.155256169441026	0.0
20	6.477103439341926E-5	0.0	0.0	2.2279292700304425	0.0
21	6.477103439341926E-5	0.0	0.0	2.323466545760736	0.0
22	1.943131031802578E-4	0.0	0.0	2.4187447373534554	0.0
23	1.943131031802578E-4	0.0	0.0	2.5413563054601984	0.0
24	1.943131031802578E-4	0.0	0.0	2.626854070859512	0.0
25	2.5908413757367703E-4	0.0	0.0	2.692078502493685	0.0
26	3.2385517196709633E-4	0.0	0.0	2.764686832048708	0.0
27	3.2385517196709633E-4	0.0	0.0	2.83917352160114	0.0
28	3.2385517196709633E-4	0.0	0.0	2.912882958740851	0.0
29	3.886262063605156E-4	0.0	0.0	2.9955955696612473	0.0
30	3.886262063605156E-4	0.0	0.0	3.1193730163870717	0.0
31	4.533972407539348E-4	0.0	0.0	3.2321393872660145	0.0
32	4.533972407539348E-4	0.0	0.0	3.3236608588639163	0.0
33	4.533972407539348E-4	0.0	0.0	3.417902713906341	0.0
34	4.533972407539348E-4	0.0	0.0	3.5210182006606647	0.0
35	4.533972407539348E-4	0.0	0.0	3.675108491482609	0.0
36	4.533972407539348E-4	0.0	0.0	3.7878100913271586	0.0
37	4.533972407539348E-4	0.0	0.0	3.9004469201373144	0.0
38	4.533972407539348E-4	0.0	0.0	4.003173780685278	0.0
39	4.533972407539348E-4	0.0	0.0	4.117300343286482	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACTC	30	2.165989E-6	45.000004	44
GCCTATA	30	2.165989E-6	45.000004	17
AATCGGT	30	2.165989E-6	45.000004	26
AAATGCG	30	2.165989E-6	45.000004	1
TATCCGC	30	2.165989E-6	45.000004	32
CTATATG	25	3.8914302E-5	45.0	1
CCGGATA	45	3.8562575E-10	45.0	20
ACGGGTA	25	3.8914302E-5	45.0	5
TCGATAG	20	7.0340396E-4	45.0	1
CGTTATT	20	7.0340396E-4	45.0	45
CTCGGTT	20	7.0340396E-4	45.0	33
ACTCGCG	20	7.0340396E-4	45.0	25
GTATCAC	20	7.0340396E-4	45.0	35
CGGCTAA	20	7.0340396E-4	45.0	38
CACGTAC	20	7.0340396E-4	45.0	42
ATACGCC	40	6.8175723E-9	45.0	2
TATACGA	20	7.0340396E-4	45.0	37
CGCCGAT	45	3.8562575E-10	45.0	38
TCGCGAT	20	7.0340396E-4	45.0	37
AATCGAC	25	3.8914302E-5	45.0	37
>>END_MODULE