FastQCFastQC Report
Thu 26 May 2016
SRR1545233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545233_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1454842
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48400.3326821744216898No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29690.2040771437723134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24260.16675350312954945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA302.1659016E-645.00000413
TATAGCG302.1659016E-645.0000041
CCGAACG551.8189894E-1245.0000041
AAACTCG207.033905E-445.019
CGTATCA207.033905E-445.022
CTATACG351.2122291E-745.01
CCGTATT207.033905E-445.030
CCGTACT351.2122291E-745.033
CTACGAC207.033905E-445.042
CGGTGAT207.033905E-445.017
CATACCG207.033905E-445.012
CTCGCGA207.033905E-445.011
CCGAATA207.033905E-445.024
GTACGTC207.033905E-445.09
GTTTGCG253.89132E-544.9999961
GCCCAAT253.89132E-544.99999624
TACGGGA3000.042.7500044
GGCGATA4650.042.5806478
AATGCGG2650.042.452832
ACGGGAA2050.041.7073175
Produced by FastQC (version 0.11.5)