##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545233_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1454842
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275596250314468	31.0	31.0	33.0	30.0	34.0
2	31.540853920906876	31.0	31.0	34.0	30.0	34.0
3	31.62324224898649	31.0	31.0	34.0	30.0	34.0
4	35.367606929137324	37.0	35.0	37.0	33.0	37.0
5	35.22503199660169	37.0	35.0	37.0	32.0	37.0
6	35.22530144166858	37.0	35.0	37.0	32.0	37.0
7	35.87616524681031	37.0	35.0	37.0	35.0	37.0
8	35.975350587898895	37.0	35.0	37.0	35.0	37.0
9	37.64368776815627	39.0	37.0	39.0	35.0	39.0
10	36.855669550370415	39.0	37.0	39.0	32.0	39.0
11	36.49117567405945	39.0	35.0	39.0	32.0	39.0
12	35.50017321468586	37.0	35.0	39.0	31.0	39.0
13	35.03665277741501	37.0	34.0	39.0	30.0	39.0
14	35.79404842587717	38.0	34.0	40.0	29.0	41.0
15	36.24185994080457	38.0	35.0	40.0	31.0	41.0
16	36.198367245377845	38.0	35.0	40.0	31.0	41.0
17	36.31002679328752	37.0	35.0	40.0	31.0	41.0
18	36.38074100142833	37.0	35.0	40.0	31.0	41.0
19	36.336895690391124	37.0	35.0	40.0	31.0	41.0
20	36.20615915680191	37.0	35.0	40.0	31.0	41.0
21	36.06054746838488	37.0	34.0	40.0	31.0	41.0
22	35.94764448648032	36.0	34.0	40.0	30.0	41.0
23	35.884298088727164	36.0	34.0	40.0	30.0	41.0
24	35.72548977827145	36.0	34.0	40.0	30.0	41.0
25	35.70158065274442	36.0	34.0	40.0	30.0	41.0
26	35.57760430342264	36.0	34.0	40.0	30.0	41.0
27	35.54894620859172	36.0	34.0	40.0	30.0	41.0
28	35.60403947645174	36.0	34.0	40.0	30.0	41.0
29	35.61008480646008	36.0	34.0	40.0	30.0	41.0
30	35.529467804751306	36.0	34.0	40.0	30.0	41.0
31	35.41056485858945	36.0	34.0	40.0	29.0	41.0
32	35.26113488612509	36.0	34.0	40.0	29.0	41.0
33	35.067589470196765	36.0	34.0	40.0	29.0	41.0
34	34.940882927493156	36.0	34.0	40.0	27.0	41.0
35	34.78174399694262	35.0	34.0	40.0	27.0	41.0
36	34.55124680205823	35.0	33.0	40.0	25.0	41.0
37	34.38088122284069	35.0	33.0	40.0	25.0	41.0
38	34.47126904502345	35.0	33.0	40.0	26.0	41.0
39	34.476420119848065	35.0	33.0	40.0	26.0	41.0
40	34.33263474659104	35.0	33.0	40.0	24.0	41.0
41	34.3641873138114	35.0	33.0	40.0	25.0	41.0
42	34.30778600012923	35.0	33.0	40.0	25.0	41.0
43	34.181520055098765	35.0	33.0	40.0	25.0	41.0
44	34.15243579715185	35.0	33.0	40.0	25.0	41.0
45	34.08706924875691	35.0	33.0	39.0	24.0	41.0
46	34.023986797191725	35.0	33.0	39.0	24.0	41.0
47	34.05682472735871	35.0	33.0	39.0	25.0	41.0
48	34.077352042352366	35.0	33.0	39.0	25.0	41.0
49	34.0761642845065	35.0	33.0	39.0	24.0	41.0
50	33.85191794022994	35.0	33.0	39.0	24.0	41.0
51	33.6655499360068	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	8.0
14	11.0
15	50.0
16	104.0
17	321.0
18	742.0
19	1513.0
20	2469.0
21	3984.0
22	5958.0
23	8917.0
24	12246.0
25	17507.0
26	22946.0
27	27455.0
28	31087.0
29	37020.0
30	44148.0
31	54975.0
32	69867.0
33	92012.0
34	156051.0
35	228813.0
36	100925.0
37	121821.0
38	168824.0
39	244894.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.53731882912371	24.355978174949584	28.89365305648311	13.213049939443597
2	32.32241026860649	25.64072249770078	28.665105901534325	13.371761332158405
3	28.815912655807296	25.87628072326754	31.144894084718477	14.162912536206681
4	27.033176111220325	27.832025745751082	30.719830744506964	14.414967398521627
5	22.515297193784615	33.330079829974665	28.97964177553301	15.174981200707705
6	21.463842809047307	40.468449494859236	27.749611297996623	10.318096398096838
7	87.74973502277223	4.6825703409717345	5.854243966011429	1.713450670244604
8	89.69736919885459	3.1443277001901233	5.611605933840239	1.5466971671150544
9	85.35435463094962	4.694049250709012	7.345814871992973	2.6057812463484007
10	53.2370525459122	23.610467665904615	12.820979872728447	10.331499915454737
11	46.462296249352164	20.734416520831818	19.912746538799404	12.89054069101662
12	42.88843736983123	21.316954006002025	23.331193352955164	12.463415271211582
13	21.176113969764415	43.81980998623906	21.912070176692726	13.092005867303804
14	14.93014361697009	44.84899391136632	25.94109875849061	14.279763713172978
15	12.964500612437641	24.194723550736093	49.87613775241573	12.96463808441054
16	14.784629533653826	18.67673603044179	47.42508121156799	19.11355322433639
17	14.8768044914843	18.67783580622501	29.567334459687032	36.87802524260366
18	19.576833773014528	22.555713953817666	38.08214225324812	19.785310019919688
19	30.626830954839086	23.256546071669636	26.261614663310517	19.85500831018076
20	33.07988083929389	22.185020778888703	25.70203499761486	19.033063384202546
21	21.59505980718181	30.782999116055215	27.2244683615128	20.397472715250178
22	21.167384499485166	26.11568816407555	23.265000598003084	29.451926738436203
23	18.0415467796503	32.784041153609806	24.90545365063698	24.26895841610292
24	17.60699787330858	23.297100303675588	42.029100067223794	17.06680175579204
25	16.24011404674872	26.133559520552748	38.21920181023094	19.407124622467595
26	16.099961370375617	36.665562308484354	27.177384210794024	20.057092110346
27	15.615853817802897	38.69588587626698	27.62080005938789	18.067460246542236
28	14.415586022399685	29.79375079905584	39.18824174721379	16.60242143133069
29	14.632929211556995	25.2863884875471	38.1568582705201	21.92382403037581
30	17.86681990209246	34.46401739845289	30.014943203454397	17.654219496000252
31	30.0241538256388	28.527840136592154	24.43344363167959	17.01456240608946
32	32.95959286300506	24.9345977088921	25.929894792699137	16.175914635403707
33	30.682094687945494	26.892679754914965	23.21702287946045	19.208202677679086
34	20.34433979772374	25.75420561133099	26.81720764179203	27.08424694915324
35	20.50683166969334	25.200743448429453	32.41472269840986	21.877702183467346
36	32.677775318556925	26.185867606241775	24.311093575797234	16.82526349940406
37	20.655095192467634	37.125131113894156	25.88590376137065	16.333869932267557
38	19.6960907095066	36.66054458147345	24.189155935833583	19.454208773186366
39	20.344064853777937	35.526538277008775	25.42372298847572	18.705673880737564
40	26.565633931382237	24.9524690653693	26.075890027920558	22.406006975327905
41	16.90912140287399	25.010619709906646	26.915225158470818	31.165033728748554
42	19.119120839238903	24.673675904325005	24.760901871131022	31.44630138530507
43	19.3896656819091	24.668039553436046	27.72349162314533	28.218803141509525
44	17.359617058072285	29.877196286607067	31.872120821367545	20.891065833953103
45	15.806664916190211	40.60798354735428	23.290501648976313	20.29484988747919
46	19.382242195372417	36.47681328969056	25.378151029458866	18.76279348547815
47	19.33708265227427	28.695349735572663	26.74249162452005	25.225075987633023
48	20.156346874780905	24.908203090095007	33.77088371108341	21.16456632404069
49	19.62948553863581	25.796134563065955	35.59032527243508	18.984054625863152
50	19.058083283270623	36.667830596037234	26.340935991674698	17.93315012901745
51	16.889050494830364	35.48316586955834	25.80018998626655	21.82759364934474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2831.0
1	3186.5
2	3542.0
3	6397.5
4	9253.0
5	6902.0
6	4551.0
7	4641.0
8	4731.0
9	5024.5
10	5318.0
11	5693.0
12	6068.0
13	6130.5
14	6193.0
15	6049.5
16	5906.0
17	6007.0
18	6108.0
19	5435.0
20	4762.0
21	5731.5
22	6701.0
23	6735.0
24	6769.0
25	7000.0
26	9893.0
27	12555.0
28	14694.5
29	16834.0
30	21316.5
31	25799.0
32	31445.0
33	37091.0
34	40529.5
35	43968.0
36	48863.0
37	53758.0
38	61831.5
39	69905.0
40	94438.0
41	118971.0
42	142512.5
43	166054.0
44	163395.5
45	160737.0
46	148939.0
47	137141.0
48	123414.0
49	109687.0
50	101479.0
51	93271.0
52	81341.0
53	69411.0
54	61940.0
55	54469.0
56	48925.0
57	43381.0
58	42638.5
59	41896.0
60	38432.5
61	34969.0
62	31492.5
63	28016.0
64	23903.5
65	19791.0
66	16528.0
67	13265.0
68	11168.0
69	9071.0
70	7409.5
71	5748.0
72	5247.0
73	4746.0
74	3370.0
75	1632.0
76	1270.0
77	1010.5
78	751.0
79	474.0
80	197.0
81	134.5
82	72.0
83	56.0
84	40.0
85	27.5
86	15.0
87	9.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1454842.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.609282613116868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7534539826561	13.867918279396857
2	7.084480256656163	2.495052300130102
3	2.4614980256415993	1.3003564315545635
4	1.2444441864855047	0.8765507750429428
5	0.8152166519050766	0.7177690207157708
6	0.5797594540219485	0.6125488844099297
7	0.4730120121859447	0.5830581541386967
8	0.3777620234390417	0.5321694584992772
9	0.3242259464393789	0.5138447689220684
>10	5.088710769640459	22.33782826054804
>50	1.54348277558473	18.921996429020606
>100	1.2459002614240733	35.67664291818199
>500	0.006442923136000379	0.7429191874326321
>1k	0.0016107307840000948	0.8213451320065072
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4840	0.3326821744216898	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2969	0.2040771437723134	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2426	0.16675350312954945	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.873598645076235E-5	0.0	0.0	0.013678461303701707	0.0
2	6.873598645076235E-5	0.0	0.0	0.05986904419861401	0.0
3	6.873598645076235E-5	0.0	0.0	0.0912813900066124	0.0
4	6.873598645076235E-5	0.0	0.0	0.13190435799901296	0.0
5	6.873598645076235E-5	0.0	0.0	0.2453874716292216	0.0
6	6.873598645076235E-5	0.0	0.0	0.3635446323380821	0.0
7	6.873598645076235E-5	0.0	0.0	0.4432783766209664	0.0
8	6.873598645076235E-5	0.0	0.0	0.6500362238648596	0.0
9	6.873598645076235E-5	0.0	0.0	0.7421424457088811	0.0
10	6.873598645076235E-5	0.0	0.0	0.9021598221662559	0.0
11	6.873598645076235E-5	0.0	0.0	1.0475364335096182	0.0
12	6.873598645076235E-5	0.0	0.0	1.1819840230073093	0.0
13	6.873598645076235E-5	0.0	0.0	1.2328486529808735	0.0
14	6.873598645076235E-5	0.0	0.0	1.2487266658509997	0.0
15	6.873598645076235E-5	0.0	0.0	1.2764960043771076	0.0
16	6.873598645076235E-5	0.0	0.0	1.3488749981097603	0.0
17	6.873598645076235E-5	0.0	0.0	1.4317705977693798	0.0
18	6.873598645076235E-5	0.0	0.0	1.5733667298579501	0.0
19	6.873598645076235E-5	0.0	0.0	1.6256060795605296	0.0
20	6.873598645076235E-5	0.0	0.0	1.6886369791358786	0.0
21	6.873598645076235E-5	0.0	0.0	1.7768252497522068	0.0
22	6.873598645076235E-5	0.0	0.0	1.8591022255337692	0.0
23	6.873598645076235E-5	0.0	0.0	1.9594567657518824	0.0
24	1.374719729015247E-4	0.0	0.0	2.0360286546580317	0.0
25	1.374719729015247E-4	0.0	0.0	2.100640481921748	0.0
26	1.374719729015247E-4	0.0	0.0	2.1632589655783927	0.0
27	1.374719729015247E-4	0.0	0.0	2.222990537804105	0.0
28	1.374719729015247E-4	0.0	0.0	2.2873961571084696	0.0
29	1.374719729015247E-4	0.0	0.0	2.361218606556588	0.0
30	1.374719729015247E-4	0.0	0.0	2.4641163782733795	0.0
31	1.374719729015247E-4	0.0	0.0	2.5560163921580488	0.0
32	1.374719729015247E-4	0.0	0.0	2.636025080386736	0.0
33	2.0620795935228706E-4	0.0	0.0	2.717958376236045	0.0
34	2.749439458030494E-4	0.0	0.0	2.8103395420258694	0.0
35	2.749439458030494E-4	0.0	0.0	2.9417627481197273	0.0
36	2.749439458030494E-4	0.0	0.0	3.0430108561617	0.0
37	2.749439458030494E-4	0.0	0.0	3.1449463240681808	0.0
38	2.749439458030494E-4	0.0	0.0	3.240833025166994	0.0
39	2.749439458030494E-4	0.0	0.0	3.3530101550546383	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	30	2.1659016E-6	45.000004	13
TATAGCG	30	2.1659016E-6	45.000004	1
CCGAACG	55	1.8189894E-12	45.000004	1
AAACTCG	20	7.033905E-4	45.0	19
CGTATCA	20	7.033905E-4	45.0	22
CTATACG	35	1.2122291E-7	45.0	1
CCGTATT	20	7.033905E-4	45.0	30
CCGTACT	35	1.2122291E-7	45.0	33
CTACGAC	20	7.033905E-4	45.0	42
CGGTGAT	20	7.033905E-4	45.0	17
CATACCG	20	7.033905E-4	45.0	12
CTCGCGA	20	7.033905E-4	45.0	11
CCGAATA	20	7.033905E-4	45.0	24
GTACGTC	20	7.033905E-4	45.0	9
GTTTGCG	25	3.89132E-5	44.999996	1
GCCCAAT	25	3.89132E-5	44.999996	24
TACGGGA	300	0.0	42.750004	4
GGCGATA	465	0.0	42.580647	8
AATGCGG	265	0.0	42.45283	2
ACGGGAA	205	0.0	41.707317	5
>>END_MODULE