FastQCFastQC Report
Thu 26 May 2016
SRR1545232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545232_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2209754
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88230.39927521344004807No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT74520.3372321081894184No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60180.2723380068550617No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51130.23138322184279336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC1900.045.025
CGGGTCC253.8919792E-545.06
AACCGGT207.0347026E-445.011
CAGCGCG750.045.01
GTACCGT207.0347026E-445.018
CCTAGCG253.8919792E-545.015
TGGCGCG253.8919792E-545.01
TCGATTG351.2126111E-745.01
TACGGAC207.0347026E-445.04
TCGATAG253.8919792E-545.01
CGCCTAC351.2126111E-745.030
ATATCGA253.8919792E-545.012
TACGCAA351.2126111E-745.030
CTACGCG551.8189894E-1245.01
ACGTGGT351.2126111E-745.029
ACGTGCG750.045.01
AACGCAC253.8919792E-545.035
CGGTGCG253.8919792E-545.013
ACCCGCG207.0347026E-445.01
GCCAGCG207.0347026E-445.01