##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545232_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2209754
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.270792585962056	31.0	31.0	34.0	30.0	34.0
2	31.526921096194418	31.0	31.0	34.0	30.0	34.0
3	31.617324824392217	31.0	31.0	34.0	30.0	34.0
4	35.35642926769224	37.0	35.0	37.0	33.0	37.0
5	35.20184328210289	37.0	35.0	37.0	32.0	37.0
6	35.19750162235253	37.0	35.0	37.0	32.0	37.0
7	35.878568836169094	37.0	35.0	37.0	35.0	37.0
8	35.99951306797046	37.0	35.0	37.0	35.0	37.0
9	37.701986736985205	39.0	38.0	39.0	35.0	39.0
10	36.85471821750294	39.0	37.0	39.0	32.0	39.0
11	36.378823163121325	38.0	35.0	39.0	32.0	39.0
12	35.210525696525494	35.0	35.0	39.0	30.0	39.0
13	34.643398314925555	35.0	33.0	39.0	27.0	39.0
14	35.34769254858233	36.0	33.0	40.0	27.0	41.0
15	35.939055207050195	37.0	34.0	40.0	30.0	41.0
16	35.97872251843418	36.0	34.0	40.0	31.0	41.0
17	36.08582720067483	36.0	34.0	40.0	31.0	41.0
18	36.19981228679754	36.0	35.0	40.0	31.0	41.0
19	36.11708543122899	36.0	35.0	40.0	31.0	41.0
20	35.96655600578164	36.0	34.0	40.0	31.0	41.0
21	35.80127471202677	36.0	34.0	40.0	31.0	41.0
22	35.70024536667883	35.0	34.0	40.0	30.0	41.0
23	35.69257663975266	35.0	34.0	40.0	30.0	41.0
24	35.53124646453859	35.0	34.0	40.0	30.0	41.0
25	35.54784650237085	35.0	34.0	40.0	30.0	41.0
26	35.427210902209026	35.0	34.0	40.0	30.0	41.0
27	35.348974139202824	35.0	34.0	40.0	30.0	41.0
28	35.47137192646783	36.0	34.0	40.0	30.0	41.0
29	35.52632419717308	36.0	34.0	40.0	30.0	41.0
30	35.46592471379167	36.0	34.0	40.0	30.0	41.0
31	35.29449748705059	35.0	34.0	40.0	30.0	41.0
32	35.120035080828	35.0	34.0	40.0	29.0	41.0
33	34.937014708424556	35.0	34.0	40.0	29.0	41.0
34	34.812357846167494	35.0	34.0	40.0	27.0	41.0
35	34.650871544977406	35.0	34.0	40.0	27.0	41.0
36	34.39161010682637	35.0	33.0	40.0	25.0	41.0
37	34.216132655490156	35.0	33.0	40.0	25.0	41.0
38	34.368671354367955	35.0	33.0	40.0	27.0	41.0
39	34.405768696425035	35.0	33.0	40.0	27.0	41.0
40	34.24542098351219	35.0	33.0	40.0	24.0	41.0
41	34.331157676374836	35.0	33.0	40.0	25.0	41.0
42	34.295418404039545	35.0	33.0	40.0	26.0	41.0
43	34.17279660993939	35.0	33.0	40.0	26.0	41.0
44	34.16188136779026	35.0	33.0	40.0	25.0	41.0
45	34.10526873127054	35.0	33.0	39.0	25.0	41.0
46	34.039846969391164	35.0	33.0	39.0	25.0	41.0
47	34.08362921845599	35.0	33.0	39.0	26.0	41.0
48	34.132424242698505	35.0	33.0	39.0	26.0	41.0
49	34.17235674197218	35.0	33.0	39.0	26.0	41.0
50	33.94515498105219	35.0	33.0	39.0	24.0	41.0
51	33.74069557063818	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	4.0
14	11.0
15	58.0
16	143.0
17	460.0
18	1059.0
19	2051.0
20	3705.0
21	5973.0
22	9250.0
23	13572.0
24	19393.0
25	27336.0
26	36036.0
27	42020.0
28	47318.0
29	55764.0
30	67881.0
31	85275.0
32	109144.0
33	144794.0
34	255952.0
35	384929.0
36	137538.0
37	160070.0
38	228657.0
39	371069.0
40	287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.692400149518903	25.19936608328348	31.02725461748231	14.080979149715308
2	28.168293846283344	27.495775547866412	30.979511746556405	13.35641885929384
3	26.508063793526336	26.480549418623067	33.36000296865624	13.651383819194354
4	24.333794621482756	28.9794701129628	32.44320408516061	14.243531180393838
5	22.226455976547616	32.79197593940321	30.86456682508551	14.117001258963668
6	20.611479829881514	41.365328448325016	27.69244902373749	10.330742698055982
7	88.5404438684125	4.542406077780604	5.537267949283042	1.379882104523852
8	91.29939350715057	2.922859286599323	4.718036487319402	1.0597107189307045
9	87.52680162588233	4.778586213669033	6.001754041400083	1.6928581190485454
10	57.36493745457639	24.80850809637634	11.516304529825492	6.310249919221778
11	49.9024778323741	17.806914253803818	21.73730650561103	10.55330140821105
12	46.970794034087056	21.060851117364194	22.574096483137943	9.394258365410812
13	19.36858129909483	50.127208730021536	19.920316922155134	10.583893048728502
14	11.173822968529528	50.172326874394166	25.626562956781612	13.027287200294694
15	9.047341921317939	24.89833709996678	53.33104951953928	12.723271459176
16	10.782331426937116	18.005669409355068	51.99592352813933	19.216075635568483
17	11.118115410131626	19.27562977598411	30.021305538987598	39.584949274896665
18	16.869932128191646	23.589639389723924	40.83984009079744	18.700588391286992
19	29.2516271041935	24.10648425118814	26.500099106054336	20.14178953856402
20	31.727830337675595	22.598262068990486	27.503966504868867	18.16994108846505
21	17.200240388749155	33.662072791813024	29.746297551673173	19.391389267764648
22	19.475878310436364	26.293560278655452	22.82629650178255	31.40426490912563
23	14.258419715497745	34.91275499444735	26.135216861243375	24.693608428811533
24	13.601151983433448	22.83720269315046	49.34006228747634	14.221583035939748
25	10.852022442317107	27.871744999669644	43.00066885273202	18.275563705281222
26	11.804798181155006	40.75290733719681	28.036876502995355	19.405417978652828
27	11.184502890366982	44.33629263709897	28.074437245050802	16.40476722748324
28	9.663157075402964	32.53298783484496	41.87832672777151	15.925528361980565
29	10.135291077649368	25.009163915983407	41.377094463908655	23.478450542458575
30	14.598593327583071	38.913833847568554	30.383925088494014	16.10364773635436
31	29.157725249054874	31.084093523532484	24.68292850697408	15.075252720438565
32	31.819333735791407	25.396989891182457	27.275524786921984	15.508151586104155
33	30.86986153209814	27.87903087855028	22.991473259014352	18.259634330337224
34	16.82363738226065	26.50503178181825	27.250092091698896	29.421238744222205
35	17.78962726167709	25.962437447788307	34.68177905775937	21.56615623277523
36	32.16444002364064	26.690210765542226	24.79113059643743	16.3542186143797
37	15.848551467719938	40.220133100788594	29.01997235891416	14.91134307257731
38	16.00182644764983	38.30100545128553	26.3992734032838	19.29789469778084
39	16.77652806602002	39.73731917670474	26.730486741963134	16.755666015312112
40	25.551169949234172	26.116119712873015	25.98031274069421	22.352397597198603
41	13.544946632068546	24.73646387787962	28.483125270957764	33.23546421909407
42	15.788137503088578	24.37216993384784	26.43072486801698	33.4089676950466
43	16.545325859801586	25.68978266359061	28.112541033979348	29.652350442628457
44	14.132523348752848	31.38779248730854	32.85157533372493	21.62810883021368
45	12.28838142164241	46.659266144557265	21.58448406474205	19.467868369058277
46	17.36912796628041	40.505821009940476	24.79266922924452	17.332381794534594
47	16.417574082906967	30.133082687032132	28.091950506707985	25.357392723352916
48	17.760800523497185	25.40011241070273	37.380359985772174	19.458727080027913
49	18.69945704363472	25.871658112169953	37.71510312912659	17.713781715068734
50	17.18141476381534	40.07762855050834	26.166215786915647	16.574740898760677
51	14.479937585812719	38.243351974925716	24.987758818402412	22.288951620859155
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6654.0
1	5329.0
2	4004.0
3	8851.5
4	13699.0
5	9940.5
6	6182.0
7	6718.5
8	7255.0
9	8353.0
10	9451.0
11	10263.5
12	11076.0
13	11107.5
14	11139.0
15	10434.0
16	9729.0
17	9255.0
18	8781.0
19	8519.5
20	8258.0
21	8478.5
22	8699.0
23	9274.5
24	9850.0
25	13069.0
26	21113.0
27	25938.0
28	32414.5
29	38891.0
30	45069.5
31	51248.0
32	59896.0
33	68544.0
34	83648.0
35	98752.0
36	99288.0
37	99824.0
38	115026.0
39	130228.0
40	175617.5
41	221007.0
42	257715.5
43	294424.0
44	291683.5
45	288943.0
46	258102.5
47	227262.0
48	197556.5
49	167851.0
50	148433.0
51	129015.0
52	108687.0
53	88359.0
54	70021.5
55	51684.0
56	42188.0
57	32692.0
58	27659.5
59	22627.0
60	19533.5
61	16440.0
62	13404.0
63	10368.0
64	8077.0
65	5786.0
66	4543.0
67	3300.0
68	2683.0
69	2066.0
70	1721.0
71	1376.0
72	1203.0
73	1030.0
74	734.5
75	348.0
76	257.0
77	253.5
78	250.0
79	145.5
80	41.0
81	41.0
82	41.0
83	22.0
84	3.0
85	3.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2209754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.48701740702996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0248593326954	12.083723546665176
2	9.075882968585686	2.8111671503730653
3	3.264179731441943	1.5165722496154725
4	1.6338261928891717	1.0121237875734437
5	0.9791472044086754	0.7582034899360939
6	0.6226777259306139	0.5786052468274349
7	0.44955689888212846	0.4873606862906551
8	0.31306959828742764	0.38788114546314145
9	0.2597588641810316	0.3620601046081771
>10	2.6726545838490505	9.679628896723266
>50	1.068930357870145	12.087157002527876
>100	1.601277404834708	53.337313841827125
>500	0.03145690406169992	3.242234133491179
>1k	0.0015123511568124958	0.37217254010153167
>5k	0.0012098809254499968	1.28379617797641
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8823	0.39927521344004807	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	7452	0.3372321081894184	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6018	0.2723380068550617	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5113	0.23138322184279336	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3576171827271271E-4	0.0	0.0	0.028374199118996957	0.0
2	1.3576171827271271E-4	0.0	0.0	0.09811046840508038	0.0
3	1.3576171827271271E-4	0.0	0.0	0.14702994088934787	0.0
4	1.3576171827271271E-4	0.0	0.0	0.2073533977085232	0.0
5	1.8101562436361693E-4	0.0	0.0	0.38207872912550445	0.0
6	1.8101562436361693E-4	0.0	0.0	0.5395170684157603	0.0
7	1.8101562436361693E-4	0.0	0.0	0.6522445484882028	0.0
8	1.8101562436361693E-4	0.0	0.0	0.9173871842748107	0.0
9	1.8101562436361693E-4	0.0	0.0	1.0321510901213438	0.0
10	1.8101562436361693E-4	0.0	0.0	1.2384183940836853	0.0
11	1.8101562436361693E-4	0.0	0.0	1.4691680612412061	0.0
12	1.8101562436361693E-4	0.0	0.0	1.6684210097594574	0.0
13	1.8101562436361693E-4	0.0	0.0	1.7409630212231768	0.0
14	1.8101562436361693E-4	0.0	0.0	1.7658074156670833	0.0
15	1.8101562436361693E-4	0.0	0.0	1.8044542514687156	0.0
16	1.8101562436361693E-4	0.0	0.0	1.9061397784549774	0.0
17	1.8101562436361693E-4	0.0	0.0	2.038778977207418	0.0
18	1.8101562436361693E-4	0.0	0.0	2.2450462811697594	0.0
19	1.8101562436361693E-4	0.0	0.0	2.333065128516568	0.0
20	1.8101562436361693E-4	0.0	0.0	2.4269217297491035	0.0
21	1.8101562436361693E-4	0.0	0.0	2.5543567293010896	0.0
22	1.8101562436361693E-4	0.0	0.0	2.697721103797074	0.0
23	1.8101562436361693E-4	0.0	0.0	2.8597300876025114	0.0
24	2.2626953045452118E-4	0.0	0.0	2.9758968645378627	0.0
25	2.2626953045452118E-4	0.0	0.0	3.068441102493762	0.0
26	2.2626953045452118E-4	0.0	0.0	3.1656464927770243	0.0
27	2.2626953045452118E-4	0.0	0.0	3.2561995588649233	0.0
28	2.2626953045452118E-4	0.0	0.0	3.35761356241464	0.0
29	2.2626953045452118E-4	0.0	0.0	3.4726037377916277	0.0
30	2.2626953045452118E-4	0.0	0.0	3.6385498114269734	0.0
31	2.2626953045452118E-4	0.0	0.0	3.779968267961049	0.0
32	2.2626953045452118E-4	0.0	0.0	3.899529087853218	0.0
33	2.2626953045452118E-4	0.0	0.0	4.012799614798752	0.0
34	2.2626953045452118E-4	0.0	0.0	4.146434399485192	0.0
35	2.2626953045452118E-4	0.0	0.0	4.34604937925217	0.0
36	2.2626953045452118E-4	0.0	0.0	4.482942445177155	0.0
37	2.2626953045452118E-4	0.0	0.0	4.633094905586776	0.0
38	2.2626953045452118E-4	0.0	0.0	4.771707619943215	0.0
39	2.7152343654542543E-4	0.0	0.0	4.917289435837654	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	190	0.0	45.0	25
CGGGTCC	25	3.8919792E-5	45.0	6
AACCGGT	20	7.0347026E-4	45.0	11
CAGCGCG	75	0.0	45.0	1
GTACCGT	20	7.0347026E-4	45.0	18
CCTAGCG	25	3.8919792E-5	45.0	15
TGGCGCG	25	3.8919792E-5	45.0	1
TCGATTG	35	1.2126111E-7	45.0	1
TACGGAC	20	7.0347026E-4	45.0	4
TCGATAG	25	3.8919792E-5	45.0	1
CGCCTAC	35	1.2126111E-7	45.0	30
ATATCGA	25	3.8919792E-5	45.0	12
TACGCAA	35	1.2126111E-7	45.0	30
CTACGCG	55	1.8189894E-12	45.0	1
ACGTGGT	35	1.2126111E-7	45.0	29
ACGTGCG	75	0.0	45.0	1
AACGCAC	25	3.8919792E-5	45.0	35
CGGTGCG	25	3.8919792E-5	45.0	13
ACCCGCG	20	7.0347026E-4	45.0	1
GCCAGCG	20	7.0347026E-4	45.0	1
>>END_MODULE