FastQCFastQC Report
Thu 26 May 2016
SRR1545231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545231_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2687516
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102900.382881441450023No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98730.36736525475569265No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC89380.332574764206055No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34340.12777598347321467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG1050.045.0000041
GTTAGTC406.8212103E-945.00000427
GCACCGT207.0349727E-445.00000427
ATGTACG1850.045.0000041
GCCGAAT207.0349727E-445.00000441
TCTACGA207.0349727E-445.00000410
TACGATA850.045.00000432
CTACGAT850.045.00000431
CTACGAC207.0349727E-445.00000411
TACGACA207.0349727E-445.00000412
ACGGATA207.0349727E-445.00000425
GTCCGCT207.0349727E-445.00000420
ACTCGAT207.0349727E-445.00000420
TCGAGGT207.0349727E-445.00000416
ATACCCG207.0349727E-445.00000415
AATCGAT207.0349727E-445.00000411
CAAGTCG351.2127384E-745.01
CACGGAC253.8922044E-545.034
GACGTAT750.045.09
CGCATGT351.2127384E-745.038