##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545231_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2687516
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.219767621848575	31.0	31.0	34.0	30.0	34.0
2	31.458663687955717	31.0	31.0	34.0	30.0	34.0
3	31.539017814219523	31.0	31.0	34.0	30.0	34.0
4	35.274280413586375	37.0	35.0	37.0	33.0	37.0
5	35.12429023678371	37.0	35.0	37.0	32.0	37.0
6	35.10882874743815	37.0	35.0	37.0	32.0	37.0
7	35.829559340297884	37.0	35.0	37.0	35.0	37.0
8	35.95381832145372	37.0	35.0	37.0	35.0	37.0
9	37.64975166659473	39.0	37.0	39.0	35.0	39.0
10	36.841878894860535	39.0	37.0	39.0	32.0	39.0
11	36.37799254032348	38.0	35.0	39.0	32.0	39.0
12	34.814265663906745	35.0	34.0	39.0	30.0	39.0
13	34.04084440799608	35.0	33.0	39.0	25.0	39.0
14	34.68741358190984	35.0	33.0	40.0	25.0	41.0
15	35.379138952102984	35.0	33.0	40.0	30.0	41.0
16	35.49393789655578	35.0	34.0	40.0	30.0	41.0
17	35.6209529543266	35.0	34.0	40.0	31.0	41.0
18	35.737027798160085	36.0	34.0	40.0	31.0	41.0
19	35.62018979607935	36.0	34.0	40.0	30.0	41.0
20	35.43399816038305	35.0	34.0	40.0	30.0	41.0
21	35.21997710897349	35.0	34.0	40.0	30.0	41.0
22	35.080470590686716	35.0	34.0	39.0	29.0	41.0
23	35.10951116198006	35.0	34.0	39.0	29.0	41.0
24	34.98576268941282	35.0	34.0	39.0	29.0	41.0
25	34.964384584128986	35.0	34.0	39.0	29.0	41.0
26	34.81100689261013	35.0	34.0	39.0	29.0	41.0
27	34.709350939678124	35.0	34.0	39.0	29.0	41.0
28	34.82343733023357	35.0	34.0	39.0	29.0	41.0
29	34.9175204910408	35.0	34.0	39.0	29.0	41.0
30	34.855035281650416	35.0	34.0	39.0	29.0	41.0
31	34.62999699350627	35.0	34.0	39.0	29.0	41.0
32	34.438853945427674	35.0	34.0	39.0	28.0	41.0
33	34.215539181906266	35.0	33.0	39.0	27.0	41.0
34	34.08982569778189	35.0	33.0	39.0	26.0	41.0
35	33.904696753433285	35.0	33.0	39.0	25.0	41.0
36	33.61650386453513	35.0	33.0	39.0	23.0	41.0
37	33.42101628418212	35.0	33.0	38.0	23.0	41.0
38	33.58354815375983	35.0	33.0	38.0	24.0	41.0
39	33.622923919336664	35.0	33.0	38.0	23.0	41.0
40	33.399899014554705	35.0	32.0	38.0	23.0	41.0
41	33.52335911674572	35.0	33.0	38.0	23.0	41.0
42	33.48592194427866	35.0	33.0	38.0	23.0	41.0
43	33.318389546332	35.0	33.0	38.0	23.0	41.0
44	33.294111737381286	35.0	33.0	38.0	23.0	40.0
45	33.233591539548044	35.0	32.0	38.0	23.0	40.0
46	33.196502644077285	35.0	32.0	38.0	23.0	40.0
47	33.247038157168184	35.0	32.0	38.0	23.0	40.0
48	33.27995591468107	35.0	33.0	38.0	23.0	40.0
49	33.31371645787411	35.0	32.0	38.0	24.0	40.0
50	33.04847710674095	35.0	32.0	38.0	24.0	40.0
51	32.834537543218346	35.0	32.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	7.0
13	7.0
14	24.0
15	87.0
16	282.0
17	770.0
18	1897.0
19	3681.0
20	6354.0
21	10042.0
22	14688.0
23	20781.0
24	29085.0
25	40089.0
26	51198.0
27	60836.0
28	68046.0
29	77803.0
30	92894.0
31	114311.0
32	145004.0
33	191838.0
34	344307.0
35	520117.0
36	155090.0
37	173881.0
38	232724.0
39	331368.0
40	301.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.78454900361523	23.503115888426336	29.851580418497974	13.860754689460453
2	29.277741974373363	26.134318828241394	31.48922648274466	13.09871271464058
3	27.566496348300813	26.06875642786871	32.45342539356045	13.911321830270035
4	24.69708087319294	28.50613726578744	31.650044129969828	15.146737731049786
5	23.017835056609893	32.609405860281385	29.80908764822237	14.563671434886341
6	20.72947658730218	40.51082858669493	28.21255761826162	10.547137207741276
7	87.28294082714298	5.379130766105207	5.806253804628512	1.531674602123299
8	89.53795996005233	3.605783184174531	5.4484884927196715	1.4077683630534665
9	86.96097065096544	4.1071383388973315	6.781987530492842	2.1499034796444003
10	64.57680624040935	17.261478629336533	10.36942663783211	7.792288492421999
11	59.69021207687694	15.961877064173757	14.804823487562494	9.543087371386811
12	50.93874789954739	20.511245328399905	19.50291644775324	9.047090324299464
13	18.813022880607967	52.30781881856703	19.383549716541225	9.495608584283776
14	11.808673883243857	52.422013487547616	25.82898111118222	9.940331518026312
15	9.64861232454058	20.41260405519446	59.45679951300755	10.481984107257409
16	11.403727456878396	14.71637750249673	56.15099593825674	17.728899102368135
17	12.171946139111357	14.331858861491428	29.06873856750992	44.42745643188729
18	18.84747848943039	20.47232462988127	42.07744251569107	18.602754364997267
19	34.583087133248696	19.233411075506154	26.23872750897111	19.944774282274043
20	35.97578581857746	20.901122077040657	24.44379866017542	18.679293444206472
21	19.743621991459772	31.924833191690766	29.072831566398115	19.258713250451347
22	20.46763628570025	25.204240644520816	21.140711348323133	33.187411721455796
23	15.240355778346995	34.71164450741875	23.801681552779595	26.246318161454667
24	15.240579032831805	21.888837126923153	48.57809218624187	14.292491654003175
25	13.048443246477417	23.528864572341153	45.007769256071406	18.414922925110027
26	12.574883275113525	40.08805901062543	27.57591024574365	19.761147468517397
27	13.646058293234347	44.84244931006922	26.89773753905093	14.613754857645498
28	10.64071804595768	31.817931502547335	43.50374100098381	14.03760945051118
29	11.434238903135832	24.345715523182	42.2500926506112	21.96995292307097
30	16.154248011918813	37.67345013015736	30.59669226155305	15.575609596370777
31	34.67328194511214	27.13993144598953	23.028625690042404	15.158160918855925
32	37.7061197031013	22.528944943955683	25.993668502810774	13.77126685013224
33	35.83967500100464	25.383253532258042	21.002256358659817	17.774815108077497
34	19.817965734901673	25.486620358725304	25.358025775474452	29.337388130898567
35	19.341056946265624	23.21571294831361	34.1242991669631	23.31893093845767
36	38.68855106350995	22.45326167360492	23.773886369420687	15.084300893464448
37	18.749506979679374	39.6032618968594	27.069866746839832	14.577364376621386
38	18.56900572871008	41.206936070334095	20.10577797490322	20.118280226052608
39	19.844309764109312	37.877021011223746	24.642978869707193	17.635690354959745
40	28.127683704952826	22.951342429217167	23.52607389128102	25.39489997454899
41	14.75503773744975	22.909928722284818	26.136923463897517	36.19811007636792
42	18.84115294569409	21.98308772859399	24.23926034300819	34.93649898270373
43	19.504181556500498	22.14546815721283	26.763822057245427	31.58652822904124
44	15.056840591832756	29.64428862935142	33.06975660796066	22.22911417085517
45	13.954893663888884	45.905401121332865	21.456504817087602	18.683200397690655
46	20.497180295856843	38.59597487047519	24.109623905494885	16.79722092817308
47	19.346005754012253	27.834364520992622	25.53194101914184	27.287688705853284
48	20.815429563954226	23.970871243185158	35.978650917799186	19.23504827506143
49	21.401063286692988	23.518557656959064	37.082309463459936	17.998069592888005
50	18.71006535402952	41.052146294198806	23.459506845726686	16.778281506044987
51	16.287233266704273	39.5351692789922	22.566749370050264	21.610848084253263
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11219.0
1	9304.0
2	7389.0
3	12458.0
4	17527.0
5	12806.5
6	8086.0
7	8661.5
8	9237.0
9	10335.5
10	11434.0
11	12179.0
12	12924.0
13	13226.5
14	13529.0
15	12820.0
16	12111.0
17	11067.0
18	10023.0
19	9158.0
20	8293.0
21	8464.0
22	8635.0
23	8373.5
24	8112.0
25	9157.0
26	14611.5
27	19021.0
28	18781.5
29	18542.0
30	24912.0
31	31282.0
32	36986.0
33	42690.0
34	50033.5
35	57377.0
36	58248.5
37	59120.0
38	83063.5
39	107007.0
40	174137.5
41	241268.0
42	319436.5
43	397605.0
44	386866.0
45	376127.0
46	337236.0
47	298345.0
48	258438.5
49	218532.0
50	196402.0
51	174272.0
52	153178.5
53	132085.0
54	108065.0
55	84045.0
56	75124.0
57	66203.0
58	61404.0
59	56605.0
60	52554.5
61	48504.0
62	43294.0
63	38084.0
64	31713.5
65	25343.0
66	20700.5
67	16058.0
68	13616.0
69	11174.0
70	9107.5
71	7041.0
72	6058.0
73	5075.0
74	4264.0
75	2587.5
76	1722.0
77	1473.0
78	1224.0
79	941.5
80	659.0
81	426.5
82	194.0
83	115.0
84	36.0
85	63.0
86	90.0
87	49.0
88	8.0
89	5.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2687516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.95870523068182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15743726830897	11.840927709128215
2	8.805445239743992	2.6343611953248156
3	3.176956500202061	1.4256946745166355
4	1.6208373997238346	0.9698251555733457
5	0.9481692690898165	0.7091692302552798
6	0.6451802102761296	0.5790636351713963
7	0.4429783445010839	0.46384677352769965
8	0.33650886893122617	0.4026989582281885
9	0.24321382206068104	0.3274347485009904
>10	2.1285429664446363	7.4825922128090365
>50	0.915628010501299	10.0062304062131
>100	1.4348004123760352	47.810978558437675
>500	0.13223375309206578	12.004586721007717
>1k	0.011297641040875524	2.224859751274498
>5k	5.135291382216147E-4	0.7225052097715117
>10k+	2.5676456911080735E-4	0.39522506025989346
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10290	0.382881441450023	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9873	0.36736525475569265	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8938	0.332574764206055	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3434	0.12777598347321467	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.720908080175151E-5	0.0	0.0	0.03929278932664959	0.0
2	3.720908080175151E-5	0.0	0.0	0.11918068580801007	0.0
3	3.720908080175151E-5	0.0	0.0	0.1655804095677942	0.0
4	3.720908080175151E-5	0.0	0.0	0.2237382028609318	0.0
5	7.441816160350302E-5	0.0	0.0	0.4033836449717881	0.0
6	7.441816160350302E-5	0.0	0.0	0.543550252351986	0.0
7	7.441816160350302E-5	0.0	0.0	0.6581542212213807	0.0
8	7.441816160350302E-5	0.0	0.0	0.9617803205636729	0.0
9	7.441816160350302E-5	0.0	0.0	1.0840865691590302	0.0
10	7.441816160350302E-5	0.0	0.0	1.3035457277277605	0.0
11	7.441816160350302E-5	0.0	0.0	1.5224467500844645	0.0
12	1.4883632320700604E-4	0.0	0.0	1.7170874517584267	0.0
13	1.8604540400875752E-4	0.0	0.0	1.7921753768163613	0.0
14	1.8604540400875752E-4	0.0	0.0	1.8180356879735786	0.0
15	1.8604540400875752E-4	0.0	0.0	1.8651051751877943	0.0
16	1.8604540400875752E-4	0.0	0.0	1.977588226451489	0.0
17	1.8604540400875752E-4	0.0	0.0	2.106071182459937	0.0
18	1.8604540400875752E-4	0.0	0.0	2.3377349195316417	0.0
19	1.8604540400875752E-4	0.0	0.0	2.412748426427973	0.0
20	3.3488172721576355E-4	0.0	0.0	2.4990734938880363	0.0
21	3.3488172721576355E-4	0.0	0.0	2.617137907271994	0.0
22	3.3488172721576355E-4	0.0	0.0	2.7262349321827295	0.0
23	3.3488172721576355E-4	0.0	0.0	2.8723549924912075	0.0
24	3.3488172721576355E-4	0.0	0.0	2.9693962752221754	0.0
25	3.3488172721576355E-4	0.0	0.0	3.0511446257436234	0.0
26	3.3488172721576355E-4	0.0	0.0	3.1434975642935705	0.0
27	3.3488172721576355E-4	0.0	0.0	3.2254319602190273	0.0
28	3.7209080801751504E-4	0.0	0.0	3.3145104996584207	0.0
29	3.7209080801751504E-4	0.0	0.0	3.400240221825656	0.0
30	3.7209080801751504E-4	0.0	0.0	3.542974255781175	0.0
31	3.7209080801751504E-4	0.0	0.0	3.6778571736875243	0.0
32	3.7209080801751504E-4	0.0	0.0	3.785093744558172	0.0
33	4.0929988881926657E-4	0.0	0.0	3.892255897267216	0.0
34	4.0929988881926657E-4	0.0	0.0	4.0082738112070775	0.0
35	4.0929988881926657E-4	0.0	0.0	4.181965800389653	0.0
36	4.837180504227696E-4	0.0	0.0	4.306951102802737	0.0
37	4.837180504227696E-4	0.0	0.0	4.435285222487978	0.0
38	5.209271312245211E-4	0.0	0.0	4.548475246286906	0.0
39	5.209271312245211E-4	0.0	0.0	4.6663908233476565	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTACG	105	0.0	45.000004	1
GTTAGTC	40	6.8212103E-9	45.000004	27
GCACCGT	20	7.0349727E-4	45.000004	27
ATGTACG	185	0.0	45.000004	1
GCCGAAT	20	7.0349727E-4	45.000004	41
TCTACGA	20	7.0349727E-4	45.000004	10
TACGATA	85	0.0	45.000004	32
CTACGAT	85	0.0	45.000004	31
CTACGAC	20	7.0349727E-4	45.000004	11
TACGACA	20	7.0349727E-4	45.000004	12
ACGGATA	20	7.0349727E-4	45.000004	25
GTCCGCT	20	7.0349727E-4	45.000004	20
ACTCGAT	20	7.0349727E-4	45.000004	20
TCGAGGT	20	7.0349727E-4	45.000004	16
ATACCCG	20	7.0349727E-4	45.000004	15
AATCGAT	20	7.0349727E-4	45.000004	11
CAAGTCG	35	1.2127384E-7	45.0	1
CACGGAC	25	3.8922044E-5	45.0	34
GACGTAT	75	0.0	45.0	9
CGCATGT	35	1.2127384E-7	45.0	38
>>END_MODULE