##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545230_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3101967
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.208213691506067	31.0	31.0	34.0	30.0	34.0
2	31.466123269525433	31.0	31.0	34.0	30.0	34.0
3	31.555378893456957	31.0	31.0	34.0	30.0	34.0
4	35.29701412039522	37.0	35.0	37.0	33.0	37.0
5	35.13899245220855	37.0	35.0	37.0	32.0	37.0
6	35.12394039008152	37.0	35.0	37.0	32.0	37.0
7	35.84157536169791	37.0	35.0	37.0	35.0	37.0
8	35.9656179450007	37.0	35.0	37.0	35.0	37.0
9	37.6590943746339	39.0	37.0	39.0	35.0	39.0
10	36.80881518081914	39.0	37.0	39.0	32.0	39.0
11	36.3653078836751	38.0	35.0	39.0	32.0	39.0
12	35.00281079714903	35.0	34.0	39.0	30.0	39.0
13	34.328995763011015	35.0	33.0	39.0	25.0	39.0
14	34.96566823567111	36.0	33.0	40.0	25.0	41.0
15	35.61435179677927	36.0	33.0	40.0	30.0	41.0
16	35.68250178032197	35.0	34.0	40.0	31.0	41.0
17	35.808882557422436	36.0	34.0	40.0	31.0	41.0
18	35.929782940953274	36.0	35.0	40.0	31.0	41.0
19	35.85021665285285	36.0	35.0	40.0	31.0	41.0
20	35.66920215463285	35.0	34.0	40.0	31.0	41.0
21	35.495988835471174	35.0	34.0	40.0	30.0	41.0
22	35.381665891352164	35.0	34.0	40.0	30.0	41.0
23	35.3757583494602	35.0	34.0	40.0	30.0	41.0
24	35.22332668271455	35.0	34.0	40.0	29.0	41.0
25	35.2239888432082	35.0	34.0	40.0	30.0	41.0
26	35.08896451832015	35.0	34.0	40.0	29.0	41.0
27	35.017565950895026	35.0	34.0	40.0	29.0	41.0
28	35.115047323198475	35.0	34.0	40.0	29.0	41.0
29	35.19389342310863	36.0	34.0	40.0	29.0	41.0
30	35.130222532992775	36.0	34.0	40.0	29.0	41.0
31	34.930047289348984	35.0	34.0	40.0	29.0	41.0
32	34.75343096815666	35.0	34.0	40.0	29.0	41.0
33	34.58402265401276	35.0	33.0	40.0	27.0	41.0
34	34.4590751610188	35.0	34.0	40.0	27.0	41.0
35	34.28322609492622	35.0	33.0	39.0	27.0	41.0
36	34.02771241602506	35.0	33.0	39.0	24.0	41.0
37	33.815239491587114	35.0	33.0	39.0	23.0	41.0
38	33.961969937139884	35.0	33.0	39.0	25.0	41.0
39	34.031527414701706	35.0	33.0	39.0	25.0	41.0
40	33.819691827798295	35.0	33.0	39.0	23.0	41.0
41	33.93516887832785	35.0	33.0	39.0	24.0	41.0
42	33.91128048751002	35.0	33.0	39.0	24.0	41.0
43	33.76705071330546	35.0	33.0	39.0	24.0	41.0
44	33.73479472863509	35.0	33.0	39.0	24.0	41.0
45	33.68154561283212	35.0	33.0	39.0	23.0	41.0
46	33.65196567210418	35.0	33.0	39.0	23.0	41.0
47	33.7002021620475	35.0	33.0	39.0	24.0	41.0
48	33.73252810232991	35.0	33.0	39.0	24.0	41.0
49	33.77641187027457	35.0	33.0	39.0	24.0	41.0
50	33.52031340114192	35.0	33.0	39.0	24.0	40.0
51	33.318011442417024	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	5.0
14	30.0
15	76.0
16	277.0
17	838.0
18	1827.0
19	3623.0
20	6216.0
21	9912.0
22	15353.0
23	22068.0
24	30727.0
25	42246.0
26	55002.0
27	65201.0
28	73493.0
29	85132.0
30	102148.0
31	126757.0
32	160054.0
33	212745.0
34	377835.0
35	565219.0
36	184602.0
37	211796.0
38	293598.0
39	454797.0
40	387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.46168221647748	24.31125153813693	30.39084555058129	13.836220694804297
2	28.781060533525988	26.53477615977217	31.064321445070174	13.619841861631668
3	27.342618409544656	25.956691351004057	32.78893682621381	13.91175341323747
4	24.710804466972085	29.368752149845566	31.561328666617023	14.359114716565328
5	22.40001263714282	33.48269017691033	30.068276032594802	14.04902115335205
6	19.67890051699454	41.91650007882096	27.59765013618778	10.806949267996727
7	87.61053873235917	4.944959117875851	5.904060230170082	1.5404419195948893
8	90.30640880447794	3.188492978809897	5.299121492910788	1.2059767238013814
9	86.82867999562858	4.339988143007324	6.826345992720103	2.0049858686439928
10	59.420329100857614	22.682414094024857	11.041703538432227	6.855553266685301
11	54.31875967732732	17.159982681956322	19.266194643592275	9.255062997124083
12	49.686956695541895	21.12082430277305	20.28358135337997	8.908637648305092
13	19.082246845308156	51.609446522158365	19.438859278644806	9.869447353888678
14	11.292512138265815	51.06108478910317	25.81532943451687	11.831073638114137
15	9.902458665743382	23.22358684022106	56.219166741619105	10.65478775241645
16	10.79556939193744	16.66723082482825	53.93890392773359	18.59829585550072
17	11.133226111045023	17.031580284380844	30.156961695595086	41.67823190897904
18	17.48825825677707	22.27215827892431	41.020004403657424	19.219579060641202
19	30.439201964430957	22.38511886167712	26.87639810481543	20.299281069076493
20	32.154307250850835	21.825409490171882	27.599745580787932	18.420537678189355
21	18.163281556509144	31.865071420811375	29.736776696850743	20.23487032582874
22	20.49696208889392	25.488762452985476	22.833995332638935	31.18028012548167
23	14.09795784416791	33.221243166029815	25.573063801130058	27.107735188672223
24	13.820617691935471	22.744116878097024	49.51045578499062	13.924809644976882
25	11.373235111785522	27.136233235234286	43.91713387021848	17.573397782761713
26	11.6162422101847	40.45990818084138	28.478736234137887	19.445113374836033
27	11.839358703687047	44.707309910131215	27.30141874494474	16.151912641236997
28	9.8757014500799	33.598713332540285	41.93339258605911	14.592192631320708
29	10.133537848726307	25.378058502878982	41.45895169097544	23.029451957419276
30	15.1016113324223	37.13350270973225	32.57326722044432	15.19161873740114
31	30.867607553529748	30.627147226259982	23.921724505773273	14.583520714437
32	33.81425398787285	24.77202368690576	27.06569734623225	14.348024978989137
33	31.82896529847029	28.365066423981943	21.308092574808178	18.497875702739584
34	17.978334392338795	27.16785832989197	27.13610428479736	27.717702992971876
35	18.152288531760654	25.233537300686955	33.46757073818	23.146603429372394
36	34.75604350400891	25.263872890975307	24.376693884880144	15.603389720135644
37	17.303536755871356	39.2448726888455	29.34257521114828	14.109015344134868
38	16.383636576404584	39.03558612970415	25.07924810289729	19.50152919099397
39	18.35609469733237	37.29662501245178	27.650261914456216	16.69701837575964
40	25.276735697059316	25.137050136252252	25.362455500010157	24.223758666678272
41	13.836704258942795	24.064923965986743	28.092207299432907	34.006164475637554
42	17.687744582711552	22.558073635212754	26.67081887073589	33.083362911339805
43	18.201064034530347	23.78629430938498	28.749693339742173	29.262948316342502
44	14.199603026079904	31.601367777284544	32.450280741220006	21.748748455415548
45	12.996559924718735	46.4453038991066	21.74932873238174	18.808807443792922
46	19.325189468488865	38.59515591236141	24.868897702651253	17.210756916498468
47	17.990681396675075	30.44181321077884	27.185717965407115	24.381787427138974
48	18.729502925079476	25.288953750958665	36.97270151487749	19.008841809084366
49	20.678298640830157	25.81323398991672	35.606858486889124	17.901608882364
50	18.170019216838863	40.101974005526166	25.918586496890523	15.809420280744444
51	15.583112263928017	37.47373843757848	25.135696156664466	21.80745314182904
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10299.0
1	8592.0
2	6885.0
3	13456.5
4	20028.0
5	14363.5
6	8699.0
7	9491.0
8	10283.0
9	11440.5
10	12598.0
11	13853.0
12	15108.0
13	14926.0
14	14744.0
15	13571.0
16	12398.0
17	11334.5
18	10271.0
19	10381.5
20	10492.0
21	11545.5
22	12599.0
23	15277.5
24	17956.0
25	18175.5
26	23112.5
27	27830.0
28	36045.5
29	44261.0
30	52727.0
31	61193.0
32	67002.5
33	72812.0
34	90305.5
35	107799.0
36	115864.0
37	123929.0
38	147183.0
39	170437.0
40	244403.5
41	318370.0
42	363976.5
43	409583.0
44	428281.5
45	446980.0
46	390508.0
47	334036.0
48	290972.5
49	247909.0
50	212324.0
51	176739.0
52	150720.0
53	124701.0
54	101173.0
55	77645.0
56	64777.0
57	51909.0
58	44051.0
59	36193.0
60	32110.5
61	28028.0
62	24594.5
63	21161.0
64	18492.5
65	15824.0
66	12774.0
67	9724.0
68	7646.5
69	5569.0
70	4598.5
71	3628.0
72	3014.0
73	2400.0
74	1919.0
75	1100.0
76	762.0
77	455.0
78	148.0
79	132.0
80	116.0
81	88.5
82	61.0
83	42.0
84	23.0
85	12.5
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3101967.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.229414016099732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.87976091848748	11.081833615840218
2	9.228290697659848	2.6262633799584756
3	3.522744592246474	1.5037977382815453
4	1.8228826635912077	1.0375420849203978
5	1.1261541829962085	0.8012257057907797
6	0.7451629780377496	0.6361939514381382
7	0.5207904895751714	0.5187380444268668
8	0.3860871029843844	0.43950345877130714
9	0.29560109748870317	0.3785607359802194
>10	2.1469097493692435	6.797779520296701
>50	0.7341104525885708	7.630906738870073
>100	1.361266715947466	44.4816371328713
>500	0.21687421802489337	18.664452874929673
>1k	0.01242630654629119	2.200329716919331
>5k	7.033758422428974E-4	0.7734666225502671
>10k+	2.3445861408096582E-4	0.42776867815474146
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12822	0.41335062558692603	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9331	0.3008091317541418	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8224	0.26512209833309	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5629	0.1814655023731716	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03278564859007204	0.0
2	3.223760923310918E-5	0.0	0.0	0.11773174891931475	0.0
3	3.223760923310918E-5	0.0	0.0	0.16515327210121836	0.0
4	3.223760923310918E-5	0.0	0.0	0.2292094016474063	0.0
5	3.223760923310918E-5	0.0	0.0	0.41692900021180107	0.0
6	3.223760923310918E-5	0.0	0.0	0.5726366528077185	0.0
7	3.223760923310918E-5	0.0	0.0	0.6952040431119996	0.0
8	3.223760923310918E-5	0.0	0.0	0.981731913975874	0.0
9	3.223760923310918E-5	0.0	0.0	1.086278480718847	0.0
10	3.223760923310918E-5	0.0	0.0	1.2664544787226943	0.0
11	6.447521846621836E-5	0.0	0.0	1.4752897113347756	0.0
12	9.671282769932755E-5	0.0	0.0	1.6532735519107715	0.0
13	1.2895043693243673E-4	0.0	0.0	1.7240351041774462	0.0
14	1.2895043693243673E-4	0.0	0.0	1.7467626186867882	0.0
15	1.2895043693243673E-4	0.0	0.0	1.789864302231455	0.0
16	1.2895043693243673E-4	0.0	0.0	1.8946687698482931	0.0
17	1.2895043693243673E-4	0.0	0.0	2.0293897388334563	0.0
18	1.6118804616554593E-4	0.0	0.0	2.2368065166392808	0.0
19	1.6118804616554593E-4	0.0	0.0	2.318270955171348	0.0
20	1.6118804616554593E-4	0.0	0.0	2.3997353937034145	0.0
21	1.934256553986551E-4	0.0	0.0	2.5263969603803007	0.0
22	1.934256553986551E-4	0.0	0.0	2.653445378367984	0.0
23	1.934256553986551E-4	0.0	0.0	2.8169222947890806	0.0
24	1.934256553986551E-4	0.0	0.0	2.9351698454561252	0.0
25	1.934256553986551E-4	0.0	0.0	3.0322372868570167	0.0
26	1.934256553986551E-4	0.0	0.0	3.1325607267904525	0.0
27	1.934256553986551E-4	0.0	0.0	3.2251471405079424	0.0
28	2.256632646317643E-4	0.0	0.0	3.3250514915213474	0.0
29	2.256632646317643E-4	0.0	0.0	3.443524705453024	0.0
30	2.256632646317643E-4	0.0	0.0	3.6072595227479853	0.0
31	2.256632646317643E-4	0.0	0.0	3.757357831337342	0.0
32	2.5790087386487346E-4	0.0	0.0	3.878506766835366	0.0
33	2.5790087386487346E-4	0.0	0.0	4.002621562382837	0.0
34	2.5790087386487346E-4	0.0	0.0	4.133474018260027	0.0
35	2.901384830979827E-4	0.0	0.0	4.333572858769935	0.0
36	2.901384830979827E-4	0.0	0.0	4.4807053073098455	0.0
37	2.901384830979827E-4	0.0	0.0	4.630255576542239	0.0
38	2.901384830979827E-4	0.0	0.0	4.7692963851646395	0.0
39	2.901384830979827E-4	0.0	0.0	4.918588753523168	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTT	40	6.8212103E-9	45.000004	5
CTACCGT	40	6.8212103E-9	45.000004	30
CCAACCG	20	7.0351415E-4	45.000004	1
ATCGGAT	40	6.8212103E-9	45.000004	42
ACTCACG	20	7.0351415E-4	45.000004	44
TTCGATC	20	7.0351415E-4	45.000004	37
TATCGCC	20	7.0351415E-4	45.000004	38
GTTAGCG	45	3.8562575E-10	45.0	1
CCGTTAT	35	1.2128294E-7	45.0	14
TCGATTG	60	0.0	44.999996	1
GGGCGTA	60	0.0	44.999996	8
GTATACG	215	0.0	43.953487	1
ACGGGTA	230	0.0	43.04348	5
GTCGAAG	215	0.0	42.906975	1
TGCGTAG	310	0.0	42.82258	1
CGTTAGG	410	0.0	42.80488	2
TCGAACG	100	0.0	42.75	1
TTCGCGG	425	0.0	42.352943	2
CGGTCTA	395	0.0	42.151897	31
CGTTTTT	10140	0.0	42.093193	1
>>END_MODULE