FastQCFastQC Report
Thu 26 May 2016
SRR1545229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545229_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1071255
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44500.41540062823510737No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22660.21152760080466368No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20670.1929512581038128No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11750.10968443554522499No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGG302.1653632E-645.0000042
ACACAAT302.1653632E-645.00000418
CAATTCG302.1653632E-645.00000421
TAGTGAT302.1653632E-645.00000420
GCTAACG302.1653632E-645.0000041
TAATGCG1150.045.0000041
TACGTAG302.1653632E-645.0000041
ATAGGCC207.033072E-445.044
CTAAGCG406.8139343E-945.01
TCTAGAC207.033072E-445.016
CCGTAGG351.211829E-745.02
ACTACGT207.033072E-445.018
ATACGTA406.8139343E-945.044
GACTCGT207.033072E-445.011
GTCGATG650.044.9999961
CCTACCG253.8906284E-544.99999623
ACGATCC253.8906284E-544.99999614
TCGTAAG502.1827873E-1144.9999961
CGGTCTA650.044.99999631
ACGTAAG253.8906284E-544.9999961
Produced by FastQC (version 0.11.5)