FastQCFastQC Report
Thu 26 May 2016
SRR1545228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545228_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4403799
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338310.7682230728514176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87530.1987602068123454No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC55800.12670878030536817No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT51010.11583180794582132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT217300.041.5520021
CTATACG501.0822987E-940.51
ACGGGTA5250.039.4285745
GCCCTAC34200.038.7526
AGGGCGA34650.038.5064936
CGGTCTA3150.037.85714331
ACGATAG1550.037.741941
GGGCGAT66950.037.50567
ACGGGAT12700.037.381895
TCGTTAG1700.037.0588231
TAGACGG3800.036.7105262
CGACCCG16650.036.6216232
ACCCGCT16650.036.6216234
CCCTACA36400.036.4697827
TAACGGG10150.036.3546833
TCGTTGA12150.036.296324
AATCGTT12100.036.2603322
ATTATAC36000.036.2542
TAGGGTA21000.036.2142875
GGACTAA41200.036.2075238
Produced by FastQC (version 0.11.5)