##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545228_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4403799
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.134940763645208	31.0	31.0	33.0	30.0	34.0
2	31.421499255529145	31.0	31.0	34.0	30.0	34.0
3	31.495547140094267	31.0	31.0	34.0	30.0	34.0
4	35.278700049661666	37.0	35.0	37.0	33.0	37.0
5	35.14085384005946	37.0	35.0	37.0	32.0	37.0
6	35.136120426931384	37.0	35.0	37.0	32.0	37.0
7	35.79200776420541	37.0	35.0	37.0	35.0	37.0
8	35.865754090956464	37.0	35.0	37.0	35.0	37.0
9	37.46060435546672	39.0	37.0	39.0	35.0	39.0
10	36.686636924164794	39.0	35.0	39.0	32.0	39.0
11	36.385121119288144	38.0	35.0	39.0	32.0	39.0
12	35.79994114172786	37.0	35.0	39.0	31.0	39.0
13	35.549534617724376	37.0	35.0	39.0	30.0	39.0
14	36.36121289822719	38.0	35.0	40.0	30.0	41.0
15	36.67844876662173	38.0	35.0	40.0	31.0	41.0
16	36.53071223277902	38.0	35.0	40.0	31.0	41.0
17	36.587567461639374	38.0	35.0	40.0	31.0	41.0
18	36.593988281481515	38.0	35.0	40.0	31.0	41.0
19	36.51534686301532	38.0	35.0	40.0	31.0	41.0
20	36.39414015035654	38.0	35.0	40.0	31.0	41.0
21	36.28429249382181	38.0	35.0	40.0	30.0	41.0
22	36.19014332852158	38.0	34.0	40.0	30.0	41.0
23	36.11248969355777	38.0	34.0	40.0	30.0	41.0
24	35.908204257278776	37.0	34.0	40.0	30.0	41.0
25	35.867419471233816	37.0	34.0	40.0	30.0	41.0
26	35.74121934266301	37.0	34.0	40.0	30.0	41.0
27	35.72111124962788	37.0	34.0	40.0	29.0	41.0
28	35.69247892558221	37.0	34.0	40.0	29.0	41.0
29	35.62999355783495	37.0	34.0	40.0	29.0	41.0
30	35.50153424350203	37.0	34.0	40.0	29.0	41.0
31	35.3835838102511	37.0	34.0	40.0	28.0	41.0
32	35.23003910941439	37.0	34.0	40.0	27.0	41.0
33	35.01346519221245	37.0	34.0	40.0	26.0	41.0
34	34.79793287568302	37.0	34.0	40.0	25.0	41.0
35	34.61416585997681	37.0	33.0	40.0	24.0	41.0
36	34.43988020343345	37.0	33.0	40.0	24.0	41.0
37	34.26412717746655	36.0	33.0	40.0	23.0	41.0
38	34.2792670600997	36.0	33.0	40.0	23.0	41.0
39	34.2618945596745	36.0	33.0	40.0	23.0	41.0
40	34.14458402847178	36.0	33.0	40.0	23.0	41.0
41	34.09359191915889	36.0	33.0	40.0	23.0	41.0
42	34.02047482185268	36.0	33.0	40.0	23.0	41.0
43	33.88318063562846	36.0	33.0	40.0	23.0	41.0
44	33.837375184471405	35.0	33.0	40.0	23.0	41.0
45	33.784901626981615	35.0	33.0	40.0	23.0	41.0
46	33.688107245585	35.0	33.0	40.0	23.0	41.0
47	33.70752343601513	35.0	33.0	40.0	23.0	41.0
48	33.65866175999404	35.0	33.0	40.0	23.0	41.0
49	33.619465602312914	35.0	32.0	39.0	23.0	41.0
50	33.44781153726589	35.0	32.0	39.0	22.0	41.0
51	33.28469214875611	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	5.0
12	8.0
13	28.0
14	55.0
15	183.0
16	536.0
17	1398.0
18	3110.0
19	6060.0
20	10073.0
21	15793.0
22	23290.0
23	33136.0
24	45925.0
25	64194.0
26	81743.0
27	95289.0
28	104585.0
29	117345.0
30	137206.0
31	166012.0
32	202808.0
33	257542.0
34	391658.0
35	523028.0
36	349413.0
37	421014.0
38	568638.0
39	783313.0
40	403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.72283794060537	23.426387080790924	27.004524956747574	15.846250021856129
2	34.94480560988365	24.14574325485791	27.41451187940231	13.494939255856137
3	29.188298557677133	25.62948490610039	31.45958750615094	13.722629030071538
4	25.86316950433024	27.948051216688135	30.98903923635025	15.199740042631374
5	22.126918144992537	32.02412280851147	30.439036840691415	15.40992220580458
6	21.75653339309991	39.346209942824366	28.760713193313318	10.136543470762403
7	84.24521645969763	4.846065862679018	8.857284358346055	2.0514333192772876
8	85.08801151006212	4.093556495198804	8.733936312715453	2.0844956820236344
9	79.98875516343956	6.088425016673104	10.486173415271677	3.436646404615651
10	45.031641998192924	25.66699797152413	17.22941941718957	12.071940613093377
11	36.46076944020379	24.33519331831448	24.821319047486046	14.382718193995684
12	33.40458999150506	22.748971967158354	29.010542942582074	14.835895098754506
13	22.32374820013357	33.054301524660865	28.66577243875118	15.956177836454389
14	17.938965879232907	35.57535209940327	30.42482184132382	16.060860180040006
15	16.508269337451594	24.800972978103676	42.94494367249732	15.745814011947413
16	19.495848924984998	21.30094947566862	40.523148308994124	18.680053290352262
17	19.23282602135111	21.44984364636079	31.465741283832436	27.85158904845566
18	21.641064907821633	24.00847540952709	35.17160978509691	19.17884989755436
19	27.773338428933748	24.901227326678622	28.43370008485855	18.89173415952908
20	28.954954574448106	24.61165916064743	28.343368986640854	18.09001727826361
21	23.558522993442708	27.720838303473887	30.652693276872988	18.067945426210414
22	21.990694852330908	25.006114039264737	28.417827425820295	24.58536368258406
23	19.803969254727566	29.164205723285736	28.96955560414996	22.062269417836735
24	19.506203621009952	25.052914540377525	37.69300097484013	17.74788086377239
25	19.518170561372123	26.629893871178044	34.130190773920425	19.721744793529407
26	18.399886098343725	31.844686826079027	28.923209256371603	20.832217819205646
27	18.0044547900574	32.09104230233941	30.5996254597451	19.304877447858086
28	17.46962111576845	28.62351347098267	35.644156329569086	18.262709083679795
29	18.408832919031955	26.043332132097763	35.02639425641361	20.52144069245667
30	20.204895818360466	29.52614322315801	31.528346320983314	18.740614637498215
31	25.919825132800113	27.257692733024374	28.78010554069339	18.04237659348213
32	27.613476455215146	25.345366580082334	28.84441365284837	18.196743311854153
33	27.03842750316261	26.758714464488502	26.834058502670082	19.368799529678807
34	20.633026166725593	26.71218645537637	29.16513673762131	23.489650640276725
35	21.009405742632666	26.493171009848542	31.116020508656277	21.381402738862516
36	26.819798087969048	27.54912292772672	26.846525011700123	18.784553972604108
37	20.607252964996814	33.04553636530641	27.945825865349438	18.401384804347337
38	20.39093973180883	33.08956653107919	26.405133385969705	20.114360351142274
39	20.516376882777802	31.912015057907954	27.76931917192406	19.802288887390183
40	23.693179457100563	25.9219369458052	28.614384988960666	21.77049860813357
41	18.7717014332398	25.66470449718527	29.180260043657757	26.383334025917165
42	19.933925231374094	27.150149223431857	27.237846232309877	25.678079312884172
43	19.968758792124707	26.5316605049413	28.985632632188707	24.513948070745283
44	19.468213694585064	28.234508432378497	31.208463419879063	21.088814453157376
45	18.36405340025737	34.11754260355661	26.496872359524133	21.02153163666189
46	20.308488193943454	32.3916463943972	27.238323093311024	20.061542318348316
47	19.660888246716073	29.161821418279988	28.79089168238605	22.386398652617885
48	20.293682795241107	27.365554149950988	31.73478171914749	20.605981335660413
49	20.669313018146376	27.502526795614422	32.11113858738784	19.717021598851357
50	19.49509957198319	32.588953310539374	28.098375970383753	19.81757114709368
51	18.64115505725852	31.973802619056862	27.867938568495067	21.517103755189552
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10246.0
1	9815.0
2	9384.0
3	32701.5
4	56019.0
5	39991.5
6	23964.0
7	23522.0
8	23080.0
9	24119.0
10	25158.0
11	25864.5
12	26571.0
13	26298.0
14	26025.0
15	26002.0
16	25979.0
17	25378.0
18	24777.0
19	24646.5
20	24516.0
21	24621.0
22	24726.0
23	27424.5
24	30123.0
25	35189.5
26	45801.0
27	51346.0
28	59382.5
29	67419.0
30	82908.0
31	98397.0
32	110890.5
33	123384.0
34	142816.5
35	162249.0
36	167669.5
37	173090.0
38	199964.5
39	226839.0
40	268339.0
41	309839.0
42	343086.0
43	376333.0
44	375241.0
45	374149.0
46	357119.5
47	340090.0
48	318188.5
49	296287.0
50	271789.5
51	247292.0
52	223817.0
53	200342.0
54	185299.0
55	170256.0
56	164566.0
57	158876.0
58	152122.5
59	145369.0
60	138896.0
61	132423.0
62	119306.0
63	106189.0
64	95136.5
65	84084.0
66	72257.5
67	60431.0
68	51105.5
69	41780.0
70	36243.0
71	30706.0
72	27515.5
73	24325.0
74	20060.0
75	11955.5
76	8116.0
77	5898.0
78	3680.0
79	2891.5
80	2103.0
81	1483.0
82	863.0
83	654.0
84	445.0
85	365.5
86	286.0
87	211.5
88	137.0
89	82.0
90	27.0
91	21.5
92	16.0
93	11.0
94	6.0
95	5.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4403799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.56567724025896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.04730146993484	14.861323630445167
2	6.734100376863841	2.500462682007206
3	2.3844984252625205	1.3280948442998912
4	1.3293433314354908	0.9872063693168766
5	0.9116130314180068	0.8462356654660382
6	0.7009349093435612	0.7807998775981639
7	0.5728892628230605	0.744525400358798
8	0.45125667760440796	0.6702308663132026
9	0.3813324523146226	0.6371725707818752
>10	3.946252881612309	17.176667090877835
>50	1.3879021093312336	18.374910151085107
>100	1.1381944659074426	37.908355692875325
>500	0.011983838457266917	1.406148065786737
>1k	0.001892185019568455	0.5304115753224952
>5k	3.78437003913691E-4	0.45514034593859815
>10k+	1.2614566797123034E-4	0.7923151715266397
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33831	0.7682230728514176	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8753	0.1987602068123454	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5580	0.12670878030536817	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5101	0.11583180794582132	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2707666721392143E-5	0.0	0.0	0.015259552036775521	0.0
2	2.2707666721392143E-5	0.0	0.0	0.05329489379510736	0.0
3	2.2707666721392143E-5	0.0	0.0	0.0878786702117876	0.0
4	2.2707666721392143E-5	0.0	0.0	0.1312048983162038	0.0
5	2.2707666721392143E-5	0.0	0.0	0.2468323372615326	0.0
6	4.541533344278429E-5	0.0	0.0	0.4067624339802975	0.0
7	4.541533344278429E-5	0.0	0.0	0.5141469899057609	0.0
8	4.541533344278429E-5	0.0	0.0	0.754575765151861	0.0
9	4.541533344278429E-5	0.0	0.0	0.8682957600925928	0.0
10	4.541533344278429E-5	0.0	0.0	1.0393753211715613	0.0
11	4.541533344278429E-5	0.0	0.0	1.1913577345378388	0.0
12	4.541533344278429E-5	0.0	0.0	1.3177258998423862	0.0
13	4.541533344278429E-5	0.0	0.0	1.3733823909765182	0.0
14	4.541533344278429E-5	0.0	0.0	1.4028115270474424	0.0
15	4.541533344278429E-5	0.0	0.0	1.435964720460675	0.0
16	6.812300016417644E-5	0.0	0.0	1.505745380295513	0.0
17	1.1353833360696072E-4	0.0	0.0	1.5842684918180872	0.0
18	1.3624600032835287E-4	0.0	0.0	1.702098574435391	0.0
19	1.3624600032835287E-4	0.0	0.0	1.7615018305785528	0.0
20	1.3624600032835287E-4	0.0	0.0	1.827467602404197	0.0
21	1.3624600032835287E-4	0.0	0.0	1.911781168940726	0.0
22	1.8166133377113715E-4	0.0	0.0	2.001953313491374	0.0
23	1.8166133377113715E-4	0.0	0.0	2.1111771904212704	0.0
24	2.0436900049252928E-4	0.0	0.0	2.1993283526337146	0.0
25	2.0436900049252928E-4	0.0	0.0	2.2740138684803735	0.0
26	2.0436900049252928E-4	0.0	0.0	2.3483133539927685	0.0
27	2.0436900049252928E-4	0.0	0.0	2.429425139521581	0.0
28	2.2707666721392145E-4	0.0	0.0	2.512149169387613	0.0
29	2.4978433393531356E-4	0.0	0.0	2.615287391636176	0.0
30	2.4978433393531356E-4	0.0	0.0	2.7619335033229264	0.0
31	2.4978433393531356E-4	0.0	0.0	2.8721338099218423	0.0
32	2.7249200065670575E-4	0.0	0.0	2.9806991645168184	0.0
33	2.7249200065670575E-4	0.0	0.0	3.089082857778023	0.0
34	2.951996673780979E-4	0.0	0.0	3.206231710393685	0.0
35	2.951996673780979E-4	0.0	0.0	3.357987047092749	0.0
36	2.951996673780979E-4	0.0	0.0	3.480290540054167	0.0
37	3.4061500082088216E-4	0.0	0.0	3.6094517483654456	0.0
38	3.633226675422743E-4	0.0	0.0	3.7629101600686137	0.0
39	3.633226675422743E-4	0.0	0.0	4.006972161990136	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	21730	0.0	41.552002	1
CTATACG	50	1.0822987E-9	40.5	1
ACGGGTA	525	0.0	39.428574	5
GCCCTAC	3420	0.0	38.75	26
AGGGCGA	3465	0.0	38.506493	6
CGGTCTA	315	0.0	37.857143	31
ACGATAG	155	0.0	37.74194	1
GGGCGAT	6695	0.0	37.5056	7
ACGGGAT	1270	0.0	37.38189	5
TCGTTAG	170	0.0	37.058823	1
TAGACGG	380	0.0	36.710526	2
CGACCCG	1665	0.0	36.62162	32
ACCCGCT	1665	0.0	36.62162	34
CCCTACA	3640	0.0	36.46978	27
TAACGGG	1015	0.0	36.354683	3
TCGTTGA	1215	0.0	36.2963	24
AATCGTT	1210	0.0	36.26033	22
ATTATAC	3600	0.0	36.25	42
TAGGGTA	2100	0.0	36.214287	5
GGACTAA	4120	0.0	36.207523	8
>>END_MODULE