FastQCFastQC Report
Thu 26 May 2016
SRR1545227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545227_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1705663
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66210.3881775004792858No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC45170.2648237078485023No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33480.19628730880601855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA453.8562575E-1045.024
ATTACGC207.0342486E-445.010
TCTTACG551.8189894E-1245.01
CTAACCG502.1827873E-1145.01
ACCGTTG406.8175723E-945.010
ACGTAAG253.8916027E-545.01
CACCGTA253.8916027E-545.012
CGGTACC253.8916027E-545.026
TATGTCA253.8916027E-545.028
CGATAGA207.0342486E-445.010
ATACCGG406.8175723E-945.02
TACGTCA253.8916027E-545.026
ACGGTAC207.0342486E-445.025
TCGAATA302.1661235E-644.99999644
TTTAGCG1200.043.1249961
ATAGCGG1850.042.567572
ACGGGTA900.042.55
TAGGACG900.042.51
TTTACGG2400.042.1874962
TATTGCG1300.041.5384641
Produced by FastQC (version 0.11.5)