##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545227_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1705663
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.211568170265757	31.0	31.0	33.0	30.0	34.0
2	31.464240005206186	31.0	31.0	34.0	30.0	34.0
3	31.558128422789263	31.0	31.0	34.0	30.0	34.0
4	35.31020723319906	37.0	35.0	37.0	33.0	37.0
5	35.16185670909201	37.0	35.0	37.0	32.0	37.0
6	35.15508221729615	37.0	35.0	37.0	32.0	37.0
7	35.82911688885788	37.0	35.0	37.0	35.0	37.0
8	35.93691837133127	37.0	35.0	37.0	35.0	37.0
9	37.62718954447625	39.0	37.0	39.0	35.0	39.0
10	36.813824301752454	39.0	37.0	39.0	32.0	39.0
11	36.413124984243666	38.0	35.0	39.0	32.0	39.0
12	35.15692842020962	35.0	35.0	39.0	30.0	39.0
13	34.56162149263952	35.0	33.0	39.0	27.0	39.0
14	35.257627679090184	36.0	33.0	40.0	26.0	41.0
15	35.802520779309866	36.0	34.0	40.0	30.0	41.0
16	35.812017966034325	36.0	34.0	40.0	31.0	41.0
17	35.879360694345834	36.0	34.0	40.0	31.0	41.0
18	35.985363462770785	36.0	35.0	40.0	31.0	41.0
19	35.928888062882294	36.0	35.0	40.0	31.0	41.0
20	35.740514392350654	36.0	34.0	40.0	30.0	41.0
21	35.567112026232614	35.0	34.0	40.0	30.0	41.0
22	35.43747094238428	35.0	34.0	40.0	30.0	41.0
23	35.422490843736426	35.0	34.0	40.0	30.0	41.0
24	35.254081843834335	35.0	34.0	40.0	29.0	41.0
25	35.24203432917288	35.0	34.0	40.0	29.0	41.0
26	35.10612002488182	35.0	34.0	40.0	29.0	41.0
27	35.040044252586824	35.0	34.0	40.0	29.0	41.0
28	35.12825745765723	36.0	34.0	40.0	29.0	41.0
29	35.17050847676241	36.0	34.0	40.0	29.0	41.0
30	35.09227965899477	36.0	34.0	40.0	29.0	41.0
31	34.904519239732586	35.0	34.0	40.0	29.0	41.0
32	34.72556009012331	35.0	34.0	40.0	28.0	41.0
33	34.522383964475985	35.0	33.0	40.0	27.0	41.0
34	34.34497670407343	35.0	33.0	40.0	26.0	41.0
35	34.181864764610594	35.0	33.0	40.0	25.0	41.0
36	33.920411593614915	35.0	33.0	39.0	23.0	41.0
37	33.73222260200286	35.0	33.0	39.0	23.0	41.0
38	33.84071648385408	35.0	33.0	39.0	24.0	41.0
39	33.850186115311175	35.0	33.0	39.0	24.0	41.0
40	33.69662178284925	35.0	33.0	39.0	23.0	41.0
41	33.748671337773054	35.0	33.0	39.0	23.0	41.0
42	33.699345650342416	35.0	33.0	39.0	23.0	41.0
43	33.541470384243546	35.0	33.0	39.0	23.0	41.0
44	33.52142539294105	35.0	33.0	39.0	23.0	41.0
45	33.432013826881395	35.0	32.0	39.0	23.0	41.0
46	33.391138226015336	35.0	32.0	38.0	23.0	41.0
47	33.41157544016608	35.0	32.0	38.0	23.0	41.0
48	33.4301383098537	35.0	33.0	38.0	23.0	40.0
49	33.449455138559024	35.0	33.0	38.0	24.0	40.0
50	33.18605316525011	35.0	32.0	38.0	24.0	40.0
51	32.96892586636399	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	6.0
14	19.0
15	44.0
16	166.0
17	438.0
18	1021.0
19	2098.0
20	3686.0
21	5834.0
22	8993.0
23	13039.0
24	17806.0
25	24224.0
26	31232.0
27	36825.0
28	42005.0
29	48349.0
30	57460.0
31	69929.0
32	87310.0
33	115290.0
34	199100.0
35	296035.0
36	109114.0
37	128722.0
38	171694.0
39	235030.0
40	187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.36233476366668	24.3314769681936	28.481007092256792	13.825181175882925
2	30.237977842047343	26.313052461125086	30.457071531715236	12.991898165112334
3	28.319544951142166	25.98274102211281	31.701455680283853	13.996258346461172
4	25.454383427441414	27.932891784602234	31.83630060568823	14.776424182268128
5	22.501572702227815	32.10851147031975	30.678451722292156	14.71146410516028
6	20.510499436289585	40.95703547535475	27.8383830803623	10.694082007993373
7	86.99145141801165	4.855765763811491	6.6185407082172745	1.5342421099595875
8	89.37638912258751	3.25228371606818	5.855201173971646	1.5161259873726523
9	85.9477516953818	3.9832018399883213	7.171991184659572	2.8970552799703104
10	59.88322429459981	19.17670723935502	11.43285631452403	9.507212151521138
11	52.52778538316185	17.716336697225653	17.206916020339307	12.548961899273186
12	45.52898198530425	19.77981582528319	22.956527754896484	11.73467443451608
13	20.402916637108266	45.7905225123603	21.6521083004087	12.154452550122738
14	13.397136480066695	46.96537358200301	27.117431755276396	12.520058182653901
15	12.215836305295946	20.53793744719795	54.79241796298565	12.453808284520447
16	14.660516174648802	15.804528796133821	50.52715571598845	19.007799313228933
17	14.005814747696348	15.979358173332011	29.22453028529082	40.79029679368082
18	19.693808214166573	20.67026135877955	39.81190891752943	19.82402150952445
19	33.69258757445052	20.94903858499598	24.951294599226227	20.40707924132727
20	35.561831381697324	20.79730872980184	24.681839261331223	18.95902062716961
21	21.827758472805	30.855626228627813	27.62216217388781	19.694453124679377
22	21.891135587745058	23.364697481272678	23.29088454167089	31.453282389311372
23	18.20359590376294	31.778493172449657	24.434428137328418	25.583482786458987
24	17.91655209733693	20.89814928271294	45.01270180569081	16.172596814259325
25	15.427549287285942	23.091489936757732	41.8008129390155	19.680147836940826
26	14.97652232592253	37.15089088524521	27.39878862354404	20.47379816528822
27	15.52797944259798	38.66672373147568	27.436662459114142	18.368634366812202
28	13.11273094392034	29.163967325315728	40.564343601285834	17.158958129478098
29	13.72398885360121	23.908415671794486	39.82855933440545	22.539036140198856
30	18.267266159845175	34.92723943709865	29.592715559873202	17.212778843182974
31	33.78187836635959	26.571837461444613	23.37050167588791	16.275782496307887
32	36.54690287589049	22.287638296662355	25.921591779853348	15.24386704759381
33	34.30953242228975	24.91224819908739	22.023987153382585	18.754232225240273
34	20.496370033236342	25.506503922521624	25.484870106228485	28.51225593801355
35	20.626993726193273	24.337750188636324	33.03337177390844	22.001884311261954
36	36.405432960672776	24.52752976408587	23.159088284145227	15.907948991096129
37	20.76054883057204	37.31452227081199	25.665679562727224	16.259249335888743
38	19.714855748175342	38.58505460926337	21.194514977460376	20.5055746651009
39	20.851715725791085	36.0105718421517	24.605798449048848	18.531913983008366
40	27.816925148754475	23.039779839276576	24.43577658658246	24.707518425386493
41	16.206835699666346	22.75056678839841	25.75022146813292	35.29237604380232
42	19.772135527357985	22.648788183832327	24.476874974716576	33.10220131409311
43	20.328107017623058	21.91200723706852	27.271213598465817	30.488672146842603
44	17.46810477802473	28.15057839678764	32.40235615124442	21.978960673943213
45	16.3466053962594	41.98613676910386	21.960727294899403	19.706530539737333
46	20.508154307152115	36.80955733928683	24.564700060914728	18.11758829264632
47	20.090252294855432	26.8737142096651	25.65882006000013	27.37721343547934
48	21.547808682019838	23.626062123643415	35.09333320825978	19.73279598607697
49	22.089592141003234	23.449122130221504	36.214949846481986	18.246335882293277
50	19.834164193043996	37.97555554643561	24.220141962392336	17.97013829812806
51	17.8845410846105	36.06128525974944	24.183382063162536	21.870791592477527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3854.0
1	3766.0
2	3678.0
3	8184.0
4	12690.0
5	9288.0
6	5886.0
7	6065.0
8	6244.0
9	6690.0
10	7136.0
11	7673.0
12	8210.0
13	7907.0
14	7604.0
15	7103.5
16	6603.0
17	6899.5
18	7196.0
19	6039.5
20	4883.0
21	5263.5
22	5644.0
23	5289.5
24	4935.0
25	5936.0
26	8305.5
27	9674.0
28	12524.5
29	15375.0
30	19489.0
31	23603.0
32	26720.5
33	29838.0
34	33991.5
35	38145.0
36	41159.5
37	44174.0
38	58739.0
39	73304.0
40	103955.0
41	134606.0
42	169420.0
43	204234.0
44	205600.5
45	206967.0
46	183083.5
47	159200.0
48	143945.0
49	128690.0
50	117449.5
51	106209.0
52	96237.5
53	86266.0
54	77902.0
55	69538.0
56	63596.0
57	57654.0
58	56329.5
59	55005.0
60	51357.5
61	47710.0
62	43570.5
63	39431.0
64	34036.5
65	28642.0
66	23744.0
67	18846.0
68	16106.0
69	13366.0
70	11139.0
71	8912.0
72	7977.0
73	7042.0
74	5168.0
75	2986.5
76	2679.0
77	1848.5
78	1018.0
79	707.5
80	397.0
81	294.0
82	191.0
83	133.0
84	75.0
85	73.5
86	72.0
87	38.0
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1705663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.07261349103634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.89070024381326	13.468704332998302
2	7.531728375254594	2.5717257494038557
3	2.680027880603087	1.3726524045221336
4	1.376932252224993	0.9403132858231786
5	0.8073823153087915	0.6892063104382514
6	0.5063817181832114	0.5187155612081314
7	0.40636351217380295	0.4856381026142365
8	0.33203259557612497	0.4534931336557411
9	0.27798704488772935	0.42713688355952156
>10	4.425164445900157	18.865514908668295
>50	1.5397046674966604	18.121356104037556
>100	1.206096041382939	39.155502188950386
>500	0.017371753682470268	1.8133824161260754
>1k	0.0017726279267826803	0.7159107883157284
>5k	3.5452558535653604E-4	0.4007478296785963
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6621	0.3881775004792858	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4517	0.2648237078485023	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3348	0.19628730880601855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025972305197451077	0.0
2	0.0	0.0	0.0	0.08829411202564633	0.0
3	0.0	0.0	0.0	0.12481949834170056	0.0
4	0.0	0.0	0.0	0.17998866130062036	0.0
5	0.0	0.0	0.0	0.3343567867744097	0.0
6	0.0	0.0	0.0	0.46181455539576105	0.0
7	0.0	0.0	0.0	0.5648243527590151	0.0
8	0.0	0.0	0.0	0.8172188761789404	0.0
9	0.0	0.0	0.0	0.9223392897659151	0.0
10	0.0	0.0	0.0	1.11780580337382	0.0
11	0.0	0.0	0.0	1.2758088790106838	0.0
12	0.0	0.0	0.0	1.4427234453699236	0.0
13	0.0	0.0	0.0	1.500179109237874	0.0
14	0.0	0.0	0.0	1.5202299633632201	0.0
15	0.0	0.0	0.0	1.556227695623344	0.0
16	0.0	0.0	0.0	1.6404178316584226	0.0
17	0.0	0.0	0.0	1.7389718836604886	0.0
18	0.0	0.0	0.0	1.9010203070594835	0.0
19	0.0	0.0	0.0	1.9732502844934785	0.0
20	0.0	0.0	0.0	2.0470045958668273	0.0
21	0.0	0.0	0.0	2.1402821073095915	0.0
22	0.0	0.0	0.0	2.2375463382860508	0.0
23	0.0	0.0	0.0	2.35849637355093	0.0
24	0.0	0.0	0.0	2.4445626128959823	0.0
25	0.0	0.0	0.0	2.515561397532807	0.0
26	0.0	0.0	0.0	2.5930679155261034	0.0
27	0.0	0.0	0.0	2.6634217896501244	0.0
28	0.0	0.0	0.0	2.741455961699351	0.0
29	0.0	0.0	0.0	2.827346316359093	0.0
30	0.0	0.0	0.0	2.9589666891994493	0.0
31	0.0	0.0	0.0	3.0739952733922236	0.0
32	0.0	0.0	0.0	3.172432068937416	0.0
33	0.0	0.0	0.0	3.2761454050419103	0.0
34	0.0	0.0	0.0	3.3880080648991036	0.0
35	0.0	0.0	0.0	3.5454834864800375	0.0
36	5.862822843668415E-5	0.0	0.0	3.6581669415353444	0.0
37	5.862822843668415E-5	0.0	0.0	3.7767718476627565	0.0
38	5.862822843668415E-5	0.0	0.0	3.8966665748157756	0.0
39	5.862822843668415E-5	0.0	0.0	4.0203135085887425	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	45	3.8562575E-10	45.0	24
ATTACGC	20	7.0342486E-4	45.0	10
TCTTACG	55	1.8189894E-12	45.0	1
CTAACCG	50	2.1827873E-11	45.0	1
ACCGTTG	40	6.8175723E-9	45.0	10
ACGTAAG	25	3.8916027E-5	45.0	1
CACCGTA	25	3.8916027E-5	45.0	12
CGGTACC	25	3.8916027E-5	45.0	26
TATGTCA	25	3.8916027E-5	45.0	28
CGATAGA	20	7.0342486E-4	45.0	10
ATACCGG	40	6.8175723E-9	45.0	2
TACGTCA	25	3.8916027E-5	45.0	26
ACGGTAC	20	7.0342486E-4	45.0	25
TCGAATA	30	2.1661235E-6	44.999996	44
TTTAGCG	120	0.0	43.124996	1
ATAGCGG	185	0.0	42.56757	2
ACGGGTA	90	0.0	42.5	5
TAGGACG	90	0.0	42.5	1
TTTACGG	240	0.0	42.187496	2
TATTGCG	130	0.0	41.538464	1
>>END_MODULE