FastQCFastQC Report
Thu 26 May 2016
SRR1545226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545226_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1503395
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57920.3852613584586885No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT44480.29586369516993205No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39080.25994499116998526No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36720.24424718719963817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG207.033982E-445.01
TAATACG502.1827873E-1145.01
ACTATCG1000.045.01
ACGCATG207.033982E-445.01
CCGGCTA207.033982E-445.014
CGCATCG207.033982E-445.021
CACGCTA253.8913808E-545.01
TCTACCG207.033982E-445.030
CTACGTT253.8913808E-545.022
TACGATG207.033982E-445.01
GTATCGC253.8913808E-545.010
CGTAAGC207.033982E-445.032
TATAGCG1050.045.01
GCGGATA207.033982E-445.041
GGTTAAC4250.045.08
GGCTCGA207.033982E-445.08
ACGGTAC207.033982E-445.019
ATCGTAC302.1659507E-644.99999628
TATTACG302.1659507E-644.9999961
TCGTAGT302.1659507E-644.99999618