FastQCFastQC Report
Thu 26 May 2016
SRR1545225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545225_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2422658
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201860.8332170698464249No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48590.2005648341614871No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT30530.12601861261473968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126450.041.049821
GCGATCG1150.039.1304329
CGAATAT5050.038.7623814
GCGATAT2350.038.297879
AGGGCGA21700.038.260376
GGGCGAT38250.038.2352947
TAGGGAC14400.038.1250045
TTACGGG5750.037.5652163
CTATACG301.1402954E-437.4999961
CGTAGCA301.1402954E-437.49999639
TACGGGA4850.037.1134034
ACGTATA850.037.05882613
CCTATGC5100.037.05882335
GTTGATC8250.036.8181816
CGGTCTA2200.036.8181831
GGCGATA10900.036.743128
ATAGGGA21900.036.2671244
ATAGGGC11350.036.0792964
GTCGAAC250.002107567936.00000432
TAGGGCG9500.036.05
Produced by FastQC (version 0.11.5)