##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545225_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2422658
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.114740916794695	31.0	31.0	33.0	28.0	34.0
2	31.39034688346436	31.0	31.0	34.0	30.0	34.0
3	31.477237397932353	31.0	31.0	34.0	30.0	34.0
4	35.26652792098596	37.0	35.0	37.0	33.0	37.0
5	35.12556786801934	37.0	35.0	37.0	32.0	37.0
6	35.116815910458676	37.0	35.0	37.0	32.0	37.0
7	35.77692971934132	37.0	35.0	37.0	35.0	37.0
8	35.8445698897657	37.0	35.0	37.0	35.0	37.0
9	37.43312840689854	39.0	37.0	39.0	35.0	39.0
10	36.65650991596833	39.0	35.0	39.0	32.0	39.0
11	36.37750520296303	38.0	35.0	39.0	32.0	39.0
12	35.858370847226475	37.0	35.0	39.0	31.0	39.0
13	35.625048603641126	37.0	35.0	39.0	30.0	39.0
14	36.47711521807866	38.0	35.0	41.0	30.0	41.0
15	36.75696528358522	38.0	35.0	41.0	31.0	41.0
16	36.588686475763396	38.0	35.0	40.0	31.0	41.0
17	36.64148633443103	38.0	35.0	40.0	31.0	41.0
18	36.63740775627431	38.0	35.0	40.0	31.0	41.0
19	36.55098573550208	38.0	35.0	40.0	31.0	41.0
20	36.427222909713215	38.0	35.0	40.0	31.0	41.0
21	36.32252757095719	38.0	35.0	40.0	30.0	41.0
22	36.21879274746993	38.0	35.0	40.0	30.0	41.0
23	36.109619682183784	38.0	34.0	40.0	30.0	41.0
24	35.89936218814211	38.0	34.0	40.0	30.0	41.0
25	35.855397253760124	37.0	34.0	40.0	30.0	41.0
26	35.72335426626457	37.0	34.0	40.0	29.0	41.0
27	35.70136189259895	37.0	34.0	40.0	29.0	41.0
28	35.65898570908482	37.0	34.0	40.0	29.0	41.0
29	35.59512320765044	37.0	34.0	40.0	29.0	41.0
30	35.480821890667194	37.0	34.0	40.0	29.0	41.0
31	35.37232494227415	37.0	34.0	40.0	28.0	41.0
32	35.2000451570135	37.0	34.0	40.0	27.0	41.0
33	34.979527444649634	37.0	34.0	40.0	26.0	41.0
34	34.745132412416446	37.0	34.0	40.0	25.0	41.0
35	34.54794403502269	37.0	33.0	40.0	24.0	41.0
36	34.38296862371825	37.0	33.0	40.0	23.0	41.0
37	34.18999586404684	36.0	33.0	40.0	23.0	41.0
38	34.199647246949425	36.0	33.0	40.0	23.0	41.0
39	34.14619686311481	36.0	33.0	40.0	23.0	41.0
40	34.0147020338818	36.0	33.0	40.0	23.0	41.0
41	33.944287225023096	36.0	33.0	40.0	23.0	41.0
42	33.85250291209077	36.0	33.0	40.0	23.0	41.0
43	33.71157381685735	35.0	33.0	40.0	23.0	41.0
44	33.65341579372738	35.0	32.0	40.0	23.0	41.0
45	33.6264821530732	35.0	32.0	40.0	23.0	41.0
46	33.49778879230993	35.0	32.0	40.0	22.0	41.0
47	33.492706770827745	35.0	32.0	40.0	22.0	41.0
48	33.428239148901746	35.0	32.0	39.0	22.0	41.0
49	33.37315254567504	35.0	32.0	39.0	22.0	41.0
50	33.20344926935622	35.0	32.0	39.0	21.0	41.0
51	33.04958314380321	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	2.0
12	8.0
13	15.0
14	30.0
15	101.0
16	296.0
17	851.0
18	1788.0
19	3477.0
20	5787.0
21	8978.0
22	13553.0
23	18800.0
24	26478.0
25	36028.0
26	46407.0
27	53858.0
28	59105.0
29	66118.0
30	76614.0
31	92476.0
32	111534.0
33	141638.0
34	210762.0
35	276178.0
36	197577.0
37	240503.0
38	316827.0
39	416653.0
40	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.653732388145585	22.64413714193254	25.27542063304024	16.42670983688164
2	35.05348257987714	24.988834577559025	27.00141745141081	12.956265391153025
3	29.65515561833325	25.2859462623284	31.419746410760414	13.639151708577934
4	26.444591023578234	27.84846230875344	30.498568101647034	15.208378566021288
5	22.968615462851133	31.82913147460351	30.19881469031122	15.003438372234132
6	21.042590411027888	40.76406987696984	28.120931637895236	10.072408074107035
7	83.69509852401784	4.947912581965759	9.15989793028979	2.197090963726618
8	84.30092072426237	4.412054858754311	8.955411783256242	2.331612633727088
9	79.35053152364057	6.128681803209533	10.733623978291613	3.7871626948582917
10	44.00720200705176	25.338037808060403	17.49078904244842	13.16397114243942
11	35.65183364717595	24.71289798229878	24.321881173487963	15.313387197037304
12	32.40853640918363	22.466357199406602	29.173370735778636	15.951735655631131
13	22.54284343890058	31.52619973599245	29.014825864814597	16.91613096029237
14	18.58487661073086	33.543405631335496	30.725550201472927	17.146167556460714
15	17.59918238562769	24.67058908025813	40.78966160308223	16.94056693103195
16	19.807830903082483	21.60292538195651	38.87478133521116	19.714462379749843
17	19.896947897722253	21.947464313989016	30.676843367904176	27.478744420384555
18	22.058458106757122	23.7981176047135	33.90936731474273	20.234056973786643
19	27.918426785786522	24.419377394580664	27.841981823270146	19.820213996362675
20	29.264180086500037	23.77009053692267	27.989092971438804	18.97663640513849
21	23.856896020816805	26.75949308569348	29.99156298577843	19.392047907711284
22	22.901168881451696	24.576725233194285	27.993881100840483	24.52822478451354
23	21.829659819916802	27.866871840763324	27.910295221199195	22.393173118120675
24	20.77168960703492	24.54217640294255	35.99802365831248	18.688110331710046
25	21.09476451071509	25.8381083916921	32.95553891634725	20.11158818124556
26	19.856248797808025	30.375397600486735	28.385228125472107	21.38312547623313
27	19.837839265798145	30.44168842651336	29.542056699707516	20.178415607980988
28	19.402490983044242	27.422318792004486	33.87766659594544	19.297523629005827
29	19.40822848293073	25.619670626229542	34.10700148349458	20.865099407345156
30	20.797446441057716	28.632270836411912	30.861888058487825	19.708394664042554
31	26.199653438496068	26.86726727420874	27.797567795371858	19.135511491923335
32	27.758726159449665	25.71592853799422	27.667380208019456	18.85796509453666
33	26.751485352038955	26.594261344358138	26.41557330832499	20.238679995277913
34	21.512941570787127	26.475177263980303	28.661783875396363	23.350097289836206
35	21.90247240840432	26.229496693301325	30.373416305561907	21.494614592732443
36	27.123556028131084	27.256591726937934	26.409175376796888	19.21067686813409
37	21.657699931232553	32.04534028327564	27.192117087925745	19.104842697566063
38	21.615060813371098	31.613087773841787	26.110577720833895	20.66127369195322
39	21.68717169323941	30.902710989334853	26.684203878549923	20.72591343887581
40	24.463667591546145	26.506341382068786	27.77750718425795	21.252483842127116
41	19.7699799146227	25.93746207677683	28.40594916822762	25.886608840372848
42	21.04535596852713	27.30719730147631	26.509891202142438	25.137555527854115
43	20.867163256225187	26.479676454538776	28.011960417029556	24.641199872206478
44	20.133423702396293	28.100871026781327	30.386955154214917	21.378750116607463
45	19.940660217001327	32.28462292242653	26.276428616833247	21.4982882437389
46	21.347049397810174	31.17749182922228	27.524231649700454	19.95122712326709
47	21.449127363416544	28.594502401907327	27.694705567191075	22.26166466748505
48	21.567839950995975	27.024037235135957	30.381547870149234	21.02657494371884
49	21.60271899706851	27.353386239411424	30.934246600221737	20.109648163298328
50	20.16190481694073	31.358986699732277	28.1575030400494	20.32160544327759
51	19.912715703165695	31.48141421529576	26.93929560012185	21.666574481416692
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5692.0
1	5493.0
2	5294.0
3	19235.5
4	33177.0
5	23726.0
6	14275.0
7	14328.0
8	14381.0
9	14763.5
10	15146.0
11	15485.5
12	15825.0
13	15493.0
14	15161.0
15	14752.0
16	14343.0
17	13486.5
18	12630.0
19	12363.0
20	12096.0
21	12233.0
22	12370.0
23	13071.5
24	13773.0
25	15908.0
26	22022.0
27	26001.0
28	30209.5
29	34418.0
30	38054.0
31	41690.0
32	52417.5
33	63145.0
34	69903.0
35	76661.0
36	79009.5
37	81358.0
38	95242.0
39	109126.0
40	130333.0
41	151540.0
42	166689.5
43	181839.0
44	190803.5
45	199768.0
46	192122.5
47	184477.0
48	173333.5
49	162190.0
50	151140.0
51	140090.0
52	129506.5
53	118923.0
54	110888.5
55	102854.0
56	100950.0
57	99046.0
58	97454.5
59	95863.0
60	92196.0
61	88529.0
62	79613.0
63	70697.0
64	62929.0
65	55161.0
66	48328.5
67	41496.0
68	36413.0
69	31330.0
70	27161.5
71	22993.0
72	20039.5
73	17086.0
74	14370.5
75	8731.5
76	5808.0
77	4739.0
78	3670.0
79	2645.5
80	1621.0
81	1248.5
82	876.0
83	587.0
84	298.0
85	223.0
86	148.0
87	102.0
88	56.0
89	40.5
90	25.0
91	17.0
92	9.0
93	5.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2422658.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.420600457836724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.67990132629232	16.63950129914689
2	6.218139931543516	2.663925821290277
3	2.5372441024240375	1.6304787654608361
4	1.5741164405899566	1.3487407739195818
5	1.1409252197706599	1.221965164248843
6	0.85331294473063	1.0967085392744962
7	0.6688059233879255	1.0028357128109118
8	0.5205695212368757	0.8920729379953971
9	0.47794455866195784	0.9214073488885416
>10	6.331154217782349	32.67778269252855
>50	1.4367301732077407	21.454390348986514
>100	0.5559855494483827	16.342089286690207
>500	0.003181594414602108	0.5001480151832552
>1k	0.0017896468582136857	0.7481348781024267
>5k	0.0	0.0
>10k+	1.9884965091263176E-4	0.859818415473445
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20186	0.8332170698464249	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4859	0.2005648341614871	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3053	0.12601861261473968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.255395520127067E-5	0.0	0.0	0.02080359671072021	0.0
2	8.255395520127067E-5	0.0	0.0	0.06088354196093712	0.0
3	8.255395520127067E-5	0.0	0.0	0.09935368508472925	0.0
4	8.255395520127067E-5	0.0	0.0	0.14929882798149802	0.0
5	8.255395520127067E-5	0.0	0.0	0.2797753541771063	0.0
6	1.2383093280190601E-4	0.0	0.0	0.4642421670743456	0.0
7	1.2383093280190601E-4	0.0	0.0	0.5891463012938681	0.0
8	1.2383093280190601E-4	0.0	0.0	0.8576117636084004	0.0
9	1.6510791040254133E-4	0.0	0.0	0.9878406279384049	0.0
10	2.0638488800317668E-4	0.0	0.0	1.197114904373626	0.0
11	2.0638488800317668E-4	0.0	0.0	1.353430818547232	0.0
12	2.0638488800317668E-4	0.0	0.0	1.4898099525397313	0.0
13	2.0638488800317668E-4	0.0	0.0	1.5514777570750804	0.0
14	2.4766186560381203E-4	0.0	0.0	1.5828895370291638	0.0
15	2.4766186560381203E-4	0.0	0.0	1.6204928636233427	0.0
16	2.4766186560381203E-4	0.0	0.0	1.6861645349859535	0.0
17	2.4766186560381203E-4	0.0	0.0	1.7566656127278386	0.0
18	2.4766186560381203E-4	0.0	0.0	1.8726126428080232	0.0
19	2.4766186560381203E-4	0.0	0.0	1.931721274732133	0.0
20	2.4766186560381203E-4	0.0	0.0	1.9955354821027154	0.0
21	2.889388432044473E-4	0.0	0.0	2.073094923014309	0.0
22	2.889388432044473E-4	0.0	0.0	2.159239975266835	0.0
23	2.889388432044473E-4	0.0	0.0	2.260120908522788	0.0
24	2.889388432044473E-4	0.0	0.0	2.3409412306648316	0.0
25	2.889388432044473E-4	0.0	0.0	2.414620635681966	0.0
26	2.889388432044473E-4	0.0	0.0	2.48636002275187	0.0
27	2.889388432044473E-4	0.0	0.0	2.5658182046330933	0.0
28	3.3021582080508267E-4	0.0	0.0	2.6435427534550895	0.0
29	3.3021582080508267E-4	0.0	0.0	2.734145719288484	0.0
30	3.3021582080508267E-4	0.0	0.0	2.8560366341431602	0.0
31	3.3021582080508267E-4	0.0	0.0	2.952046884042238	0.0
32	3.3021582080508267E-4	0.0	0.0	3.049501828157338	0.0
33	3.3021582080508267E-4	0.0	0.0	3.145966124810023	0.0
34	3.71492798405718E-4	0.0	0.0	3.248374306237199	0.0
35	3.71492798405718E-4	0.0	0.0	3.3740627030311336	0.0
36	4.1276977600635336E-4	0.0	0.0	3.4795666577783573	0.0
37	4.1276977600635336E-4	0.0	0.0	3.592830684314501	0.0
38	4.953237312076241E-4	0.0	0.0	3.721945070249288	0.0
39	4.953237312076241E-4	0.0	0.0	3.9068659299001345	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12645	0.0	41.04982	1
GCGATCG	115	0.0	39.130432	9
CGAATAT	505	0.0	38.76238	14
GCGATAT	235	0.0	38.29787	9
AGGGCGA	2170	0.0	38.26037	6
GGGCGAT	3825	0.0	38.235294	7
TAGGGAC	1440	0.0	38.125004	5
TTACGGG	575	0.0	37.565216	3
CTATACG	30	1.1402954E-4	37.499996	1
CGTAGCA	30	1.1402954E-4	37.499996	39
TACGGGA	485	0.0	37.113403	4
ACGTATA	85	0.0	37.058826	13
CCTATGC	510	0.0	37.058823	35
GTTGATC	825	0.0	36.81818	16
CGGTCTA	220	0.0	36.81818	31
GGCGATA	1090	0.0	36.74312	8
ATAGGGA	2190	0.0	36.267124	4
ATAGGGC	1135	0.0	36.079296	4
GTCGAAC	25	0.0021075679	36.000004	32
TAGGGCG	950	0.0	36.0	5
>>END_MODULE