FastQCFastQC Report
Thu 26 May 2016
SRR1545224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545224_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3125453
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341661.0931535364633542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65540.20969760223557996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG2050.041.7073171
CGTTTTT229000.041.6200871
AGGGTAC10800.038.3333326
CGTTGAT4000.037.687525
GCCCTAC24900.037.59036326
TTGATCC15850.037.4763417
GGGCGAT51750.037.3043487
ATCCTAG25400.037.2933114
AATCCTA25550.037.16242613
GTTGATC15900.037.07547416
CTAATCC25500.037.05882311
GGGTACC18450.036.951227
ACGGGTA2450.036.7346955
ACGGGCT9800.036.7346955
ACGATAG1600.036.56251
ATATGCT15700.036.54458634
TGATCCT16250.036.41538218
TACGGGA6450.036.279074
CGTAAGG3550.036.1267622
GTAGCAT16150.036.0835929
Produced by FastQC (version 0.11.5)