FastQCFastQC Report
Thu 26 May 2016
SRR1545223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545223_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1820136
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66130.36332449882865897No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC64850.3562920572968174No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62710.34453469411076976No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT32400.1780086762747399No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19640.1079040247541942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG207.0343725E-445.00000425
TACCCCG600.045.0000046
TTAGCTA207.0343725E-445.00000418
CGACGGT1850.045.00000428
TCTTCGA207.0343725E-445.00000425
CGCATTA207.0343725E-445.00000438
GGCCGTA207.0343725E-445.0000048
CCTACGG406.8193913E-945.0000042
TATATCG302.1662036E-645.00000411
CGCGAGG600.045.0000042
ATATCGA302.1662036E-645.00000412
ACGTGTG207.0343725E-445.0000042
GTCCGCG207.0343725E-445.00000440
TCGTACC207.0343725E-445.00000430
ACGTCGT207.0343725E-445.00000429
TAAGCGA207.0343725E-445.0000041
ATACCGG406.8193913E-945.0000042
AAATCGG950.045.02
CGACGTC453.8562575E-1045.018
TCACACG253.8917067E-545.015