##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545223_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1820136
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268217320024437	31.0	31.0	34.0	30.0	34.0
2	31.50589571328736	31.0	31.0	34.0	30.0	34.0
3	31.58789233332015	31.0	31.0	34.0	30.0	34.0
4	35.32279346158749	37.0	35.0	37.0	33.0	37.0
5	35.16937360724693	37.0	35.0	37.0	32.0	37.0
6	35.159853988932696	37.0	35.0	37.0	32.0	37.0
7	35.8537147773573	37.0	35.0	37.0	35.0	37.0
8	35.97908123349024	37.0	35.0	37.0	35.0	37.0
9	37.696250170316944	39.0	37.0	39.0	35.0	39.0
10	36.90583450906965	39.0	37.0	39.0	32.0	39.0
11	36.402892421225665	38.0	35.0	39.0	32.0	39.0
12	34.88736665831564	35.0	34.0	39.0	30.0	39.0
13	34.138837427532884	35.0	33.0	39.0	25.0	39.0
14	34.75977344550078	35.0	33.0	40.0	25.0	41.0
15	35.457684480720125	35.0	33.0	40.0	30.0	41.0
16	35.5436236632867	35.0	34.0	40.0	31.0	41.0
17	35.67098942057077	35.0	34.0	40.0	31.0	41.0
18	35.78655935600417	36.0	34.0	40.0	31.0	41.0
19	35.661111037856514	36.0	34.0	40.0	31.0	41.0
20	35.4574592228273	35.0	34.0	40.0	30.0	41.0
21	35.27465475107355	35.0	34.0	40.0	30.0	41.0
22	35.14364146415433	35.0	34.0	40.0	30.0	41.0
23	35.16743474114022	35.0	34.0	39.0	30.0	41.0
24	35.025717309036246	35.0	34.0	39.0	29.0	41.0
25	35.00790820026636	35.0	34.0	39.0	29.0	41.0
26	34.8236071370491	35.0	34.0	39.0	29.0	41.0
27	34.743402141378446	35.0	34.0	39.0	29.0	41.0
28	34.897924660574816	35.0	34.0	39.0	29.0	41.0
29	34.99119186698137	35.0	34.0	39.0	29.0	41.0
30	34.941286804942045	35.0	34.0	39.0	29.0	41.0
31	34.71263356144816	35.0	34.0	39.0	29.0	41.0
32	34.5206149430592	35.0	34.0	39.0	29.0	41.0
33	34.295141132310995	35.0	33.0	39.0	27.0	41.0
34	34.17247117797791	35.0	34.0	39.0	27.0	41.0
35	33.98516484482478	35.0	33.0	39.0	25.0	41.0
36	33.65879692506494	35.0	33.0	39.0	23.0	41.0
37	33.476514392331126	35.0	33.0	39.0	23.0	41.0
38	33.62617134104265	35.0	33.0	39.0	24.0	41.0
39	33.674246869464696	35.0	33.0	39.0	24.0	41.0
40	33.479848758554304	35.0	33.0	39.0	23.0	41.0
41	33.60538333399263	35.0	33.0	38.0	23.0	41.0
42	33.57846831225798	35.0	33.0	38.0	23.0	41.0
43	33.44548484289086	35.0	33.0	38.0	23.0	41.0
44	33.40831948821407	35.0	33.0	38.0	23.0	41.0
45	33.34513629750744	35.0	33.0	38.0	23.0	40.0
46	33.31703894654026	35.0	33.0	38.0	23.0	40.0
47	33.37501648228484	35.0	33.0	38.0	23.0	40.0
48	33.413736665831564	35.0	33.0	38.0	23.0	40.0
49	33.442261457385605	35.0	33.0	38.0	24.0	40.0
50	33.1794162634001	35.0	32.0	38.0	24.0	40.0
51	32.93318411371458	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	8.0
14	10.0
15	54.0
16	160.0
17	534.0
18	1094.0
19	2186.0
20	4043.0
21	6405.0
22	9427.0
23	13634.0
24	19436.0
25	26898.0
26	34355.0
27	40134.0
28	44959.0
29	51057.0
30	61736.0
31	76413.0
32	95857.0
33	128832.0
34	232915.0
35	359932.0
36	101497.0
37	113996.0
38	156840.0
39	237505.0
40	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.425583582765242	25.080598372868838	30.05753416228238	14.436283882083536
2	28.903829164414084	27.346033483212246	31.554125625777413	12.196011726596254
3	26.76085743043377	26.872607321650687	32.55058962626969	13.815945621645856
4	24.11484636312891	29.264901084314577	32.88188355155878	13.738369000997727
5	21.588606565663227	33.184882887872114	31.285079796235003	13.941430750229653
6	20.73202222251524	40.39890425770382	28.27332682832492	10.595746691456023
7	87.10398563623816	5.260321206766967	5.775447548974362	1.8602456080204994
8	90.155076323967	3.3357946878694777	5.352237415226115	1.156891572937407
9	87.45209149206433	4.326819534364465	6.287442257062109	1.933646716509096
10	63.94230980542114	19.425196798480993	9.935521301704927	6.696972094392946
11	58.75434582910288	15.514554956332931	16.985543937376107	8.745555277188078
12	51.60795676806569	21.322967074987805	18.4664772302729	8.602598926673611
13	18.67717577148081	54.08579358905049	17.858335860617007	9.37869477885169
14	10.696947920375182	53.24618599928797	25.45348259690485	10.603383483432008
15	8.621333790442034	21.910395706694445	59.135031667963275	10.33323883490025
16	10.181766637218317	15.134473467916685	55.74561461341351	18.938145281451497
17	11.25327997468321	15.47378877182804	29.837275895867123	43.43565535762163
18	18.995558573645045	21.128970582418017	41.176153869820716	18.699316974116222
19	34.3600697969822	20.71521029197818	25.169273065309405	19.75544684573021
20	34.97551831291727	21.324285657775025	25.423924366091327	18.276271663216377
21	18.33577271148969	32.93819802476298	28.69417450124606	20.031854762501265
22	20.21925834113495	25.845870858001817	21.129574932862162	32.805295868001075
23	14.58726161122026	34.703176026406815	24.444876646580255	26.26468571579267
24	14.765819696989677	20.909536430244774	49.99313238131656	14.331511491448989
25	11.447166585354061	25.71340822883565	44.92400567869654	17.915419507113754
26	11.504305172800274	41.4542100150758	27.739685386146967	19.30179942597696
27	12.283972186693742	44.96977148960297	27.5382169244496	15.208039399253682
28	9.743557624265438	31.716860718100186	44.445195304087164	14.09438635354721
29	10.135396475867738	24.48789541001332	42.841798634827285	22.534909479291656
30	15.273583951968424	38.11572322068241	31.203877072922026	15.406815754427141
31	33.62435554266275	28.344750062632684	23.882006619285594	14.14888777541898
32	37.51324076882167	22.602926374732437	25.88910938523275	13.994723471213142
33	34.03646760461855	26.213315928040544	21.954348466268456	17.79586800107245
34	18.308741764351673	25.695387597410303	26.996444221750465	28.999426416487562
35	19.638972032859083	24.024578383153784	33.97553809165908	22.360911492328047
36	36.71538830065445	24.37076130574858	24.004689759446546	14.909160634150414
37	18.564052356527206	39.93619158128843	27.797812910683596	13.701943151500767
38	17.01636581002738	40.97501505382015	22.576280014240695	19.432339121911767
39	19.02044682375383	38.98494398220792	25.731264037412586	16.263345156625657
40	27.660845123661087	24.024908028850593	23.75970806577091	24.55453878171741
41	14.198279688990272	22.290477195110693	26.351107829305064	37.16013528659397
42	17.917122676547248	22.672151971061503	24.39729778434139	35.01342756804986
43	18.204408901312867	23.387428192179048	27.98807341868959	30.42008948781849
44	14.726976445716144	30.91708531670161	33.66231973874479	20.69361849883745
45	13.03622366680292	48.15118210946874	20.60038370759108	18.212210516137255
46	19.746711234764874	40.04766676775801	23.276722179002007	16.928899818475102
47	18.79447469859395	27.1005573209914	27.108798463411528	26.99616951700313
48	20.72273720205523	23.544064839110924	37.13096164242672	18.602236316407126
49	20.583461895155086	24.464600447439093	37.0777238623927	17.87421379501312
50	18.990449065344567	41.32075844881921	23.772344484148437	15.916448001687785
51	15.528235252750344	39.376178483366076	23.134370178931686	21.961216084951893
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6914.0
1	5508.0
2	4102.0
3	7546.0
4	10990.0
5	8187.0
6	5384.0
7	5735.0
8	6086.0
9	7051.0
10	8016.0
11	8596.0
12	9176.0
13	9131.5
14	9087.0
15	8542.0
16	7997.0
17	7706.0
18	7415.0
19	6804.0
20	6193.0
21	6539.0
22	6885.0
23	6265.5
24	5646.0
25	5837.5
26	9033.5
27	12038.0
28	15017.0
29	17996.0
30	23040.5
31	28085.0
32	32340.5
33	36596.0
34	43652.0
35	50708.0
36	52628.0
37	54548.0
38	70469.5
39	86391.0
40	135660.5
41	184930.0
42	229514.5
43	274099.0
44	264906.5
45	255714.0
46	226189.5
47	196665.0
48	171401.0
49	146137.0
50	129145.5
51	112154.0
52	95719.5
53	79285.0
54	66970.0
55	54655.0
56	45120.0
57	35585.0
58	31040.5
59	26496.0
60	23891.0
61	21286.0
62	19006.0
63	16726.0
64	13696.0
65	10666.0
66	9114.5
67	7563.0
68	6010.5
69	4458.0
70	3942.0
71	3426.0
72	2690.0
73	1954.0
74	1325.5
75	684.5
76	672.0
77	507.0
78	342.0
79	260.0
80	178.0
81	112.5
82	47.0
83	74.0
84	101.0
85	56.5
86	12.0
87	7.0
88	2.0
89	1.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1820136.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.091371785110965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.16768767138583	12.739166956878153
2	8.493747201046482	2.7335208812156915
3	3.020133626124566	1.4579427905605713
4	1.5335878985643336	0.9871013216378295
5	0.8535761931939225	0.6867605935801555
6	0.5892027622745364	0.5688648422744349
7	0.4053264128688855	0.4565580602659035
8	0.3105502784042494	0.3997743990217995
9	0.26177671104096756	0.3791111743839394
>10	2.745482908448892	10.644840644329825
>50	1.162442181654793	13.788535125062317
>100	1.4092982573211434	48.66497428015333
>500	0.04120137333997341	4.309526338104913
>1k	0.0049300788611934	1.0857680818969686
>5k	0.0010564454702557285	1.0975545106342248
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6613	0.36332449882865897	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6485	0.3562920572968174	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6271	0.34453469411076976	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3240	0.1780086762747399	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1964	0.1079040247541942	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04098594830276419	0.0
2	0.0	0.0	0.0	0.11740880901207382	0.0
3	0.0	0.0	0.0	0.1681742463200552	0.0
4	5.4940949467512315E-5	0.0	0.0	0.22729070794709846	0.0
5	5.4940949467512315E-5	0.0	0.0	0.3920586154001679	0.0
6	5.4940949467512315E-5	0.0	0.0	0.5350149659146349	0.0
7	5.4940949467512315E-5	0.0	0.0	0.63561184438965	0.0
8	5.4940949467512315E-5	0.0	0.0	0.8962517086635284	0.0
9	5.4940949467512315E-5	0.0	0.0	1.0161878013511079	0.0
10	5.4940949467512315E-5	0.0	0.0	1.239907347582818	0.0
11	5.4940949467512315E-5	0.0	0.0	1.451045416386468	0.0
12	5.4940949467512315E-5	0.0	0.0	1.6456462594003964	0.0
13	5.4940949467512315E-5	0.0	0.0	1.7194319545352654	0.0
14	5.4940949467512315E-5	0.0	0.0	1.7452542007849963	0.0
15	5.4940949467512315E-5	0.0	0.0	1.7851413300984103	0.0
16	5.4940949467512315E-5	0.0	0.0	1.8837053934431274	0.0
17	5.4940949467512315E-5	0.0	0.0	2.011168396207756	0.0
18	5.4940949467512315E-5	0.0	0.0	2.214724613984889	0.0
19	5.4940949467512315E-5	0.0	0.0	2.2949933411569243	0.0
20	5.4940949467512315E-5	0.0	0.0	2.38608543537406	0.0
21	5.4940949467512315E-5	0.0	0.0	2.505856705213237	0.0
22	5.4940949467512315E-5	0.0	0.0	2.625902679799751	0.0
23	5.4940949467512315E-5	0.0	0.0	2.7766057041891377	0.0
24	5.4940949467512315E-5	0.0	0.0	2.8836306737518513	0.0
25	5.4940949467512315E-5	0.0	0.0	2.964229046620692	0.0
26	5.4940949467512315E-5	0.0	0.0	3.0475744669629083	0.0
27	1.0988189893502463E-4	0.0	0.0	3.1317989424966046	0.0
28	1.0988189893502463E-4	0.0	0.0	3.2188803474026115	0.0
29	1.0988189893502463E-4	0.0	0.0	3.3152467727686283	0.0
30	1.0988189893502463E-4	0.0	0.0	3.4693561360250005	0.0
31	1.6482284840253696E-4	0.0	0.0	3.5969839616380312	0.0
32	1.6482284840253696E-4	0.0	0.0	3.6924163908631003	0.0
33	1.6482284840253696E-4	0.0	0.0	3.800155592768892	0.0
34	1.6482284840253696E-4	0.0	0.0	3.9173995789325633	0.0
35	1.6482284840253696E-4	0.0	0.0	4.096836719893458	0.0
36	1.6482284840253696E-4	0.0	0.0	4.221552675184712	0.0
37	2.1976379787004926E-4	0.0	0.0	4.344345697244601	0.0
38	2.1976379787004926E-4	0.0	0.0	4.466864014557154	0.0
39	2.1976379787004926E-4	0.0	0.0	4.5932831392819	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	20	7.0343725E-4	45.000004	25
TACCCCG	60	0.0	45.000004	6
TTAGCTA	20	7.0343725E-4	45.000004	18
CGACGGT	185	0.0	45.000004	28
TCTTCGA	20	7.0343725E-4	45.000004	25
CGCATTA	20	7.0343725E-4	45.000004	38
GGCCGTA	20	7.0343725E-4	45.000004	8
CCTACGG	40	6.8193913E-9	45.000004	2
TATATCG	30	2.1662036E-6	45.000004	11
CGCGAGG	60	0.0	45.000004	2
ATATCGA	30	2.1662036E-6	45.000004	12
ACGTGTG	20	7.0343725E-4	45.000004	2
GTCCGCG	20	7.0343725E-4	45.000004	40
TCGTACC	20	7.0343725E-4	45.000004	30
ACGTCGT	20	7.0343725E-4	45.000004	29
TAAGCGA	20	7.0343725E-4	45.000004	1
ATACCGG	40	6.8193913E-9	45.000004	2
AAATCGG	95	0.0	45.0	2
CGACGTC	45	3.8562575E-10	45.0	18
TCACACG	25	3.8917067E-5	45.0	15
>>END_MODULE