FastQCFastQC Report
Thu 26 May 2016
SRR1545222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545222_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4729754
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147420.3116864006034986No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC85430.1806225017199626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83200.17590766877093397No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT53770.11368455949294615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA600.045.00000441
CAATACG700.045.00000412
CGACCGT207.035518E-445.035
TATCGTG2350.043.0851061
CTACGTC650.041.5384641
ACGGGTA6850.041.386865
ACGCTAG1200.041.2500041
GCTACGA7100.040.56337710
ACGACTT3500.040.50000417
TCGACTA501.0822987E-940.49999637
GCGCTAA2350.040.21276514
CGAACTA900.040.024
ACGGGAT6750.039.6666685
ACGATAT1200.039.37500439
TACGATA1200.039.37500438
AATCGTC403.461264E-739.37540
TATGTCG2000.039.3749961
CGACGGT3000.039.028
CGCGACC11650.038.81974410
ATTGGGC9350.038.7433174
Produced by FastQC (version 0.11.5)