##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545222_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4729754
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.366270000511655	31.0	31.0	34.0	30.0	34.0
2	31.61905989190981	31.0	31.0	34.0	30.0	34.0
3	31.700168127137268	31.0	31.0	34.0	30.0	34.0
4	35.45193280665337	37.0	35.0	37.0	33.0	37.0
5	35.31683888844959	37.0	35.0	37.0	33.0	37.0
6	35.31254458477122	37.0	35.0	37.0	33.0	37.0
7	35.92363640899717	37.0	35.0	37.0	35.0	37.0
8	36.01666069736397	37.0	35.0	37.0	35.0	37.0
9	37.688733917239674	39.0	38.0	39.0	35.0	39.0
10	36.93190026373465	39.0	37.0	39.0	32.0	39.0
11	36.5635373425341	39.0	35.0	39.0	32.0	39.0
12	35.53057283740338	37.0	35.0	39.0	31.0	39.0
13	35.0391995017077	37.0	34.0	39.0	30.0	39.0
14	35.81669384919385	38.0	34.0	40.0	29.0	41.0
15	36.303680487399554	38.0	35.0	40.0	31.0	41.0
16	36.25969511310736	37.0	35.0	40.0	31.0	41.0
17	36.34859656548734	37.0	35.0	40.0	31.0	41.0
18	36.45429593167002	37.0	35.0	40.0	31.0	41.0
19	36.380280665759784	37.0	35.0	40.0	31.0	41.0
20	36.24823236049909	37.0	35.0	40.0	31.0	41.0
21	36.089268701924034	36.0	34.0	40.0	31.0	41.0
22	35.97925663787165	36.0	34.0	40.0	30.0	41.0
23	35.95374748877003	36.0	34.0	40.0	31.0	41.0
24	35.783744355414676	36.0	34.0	40.0	30.0	41.0
25	35.76094105528533	36.0	34.0	40.0	30.0	41.0
26	35.631640038784255	36.0	34.0	40.0	30.0	41.0
27	35.58116933777106	36.0	34.0	40.0	30.0	41.0
28	35.65647346563902	36.0	34.0	40.0	30.0	41.0
29	35.69914058955286	36.0	34.0	40.0	30.0	41.0
30	35.62708356502262	36.0	34.0	40.0	30.0	41.0
31	35.49921623830753	36.0	34.0	40.0	30.0	41.0
32	35.349574417612416	36.0	34.0	40.0	29.0	41.0
33	35.170560879064745	36.0	34.0	40.0	29.0	41.0
34	35.0299349606766	35.0	34.0	40.0	28.0	41.0
35	34.87896389537384	35.0	34.0	40.0	27.0	41.0
36	34.64165472453747	35.0	33.0	40.0	26.0	41.0
37	34.466972278050825	35.0	33.0	40.0	26.0	41.0
38	34.55215450951572	35.0	33.0	40.0	27.0	41.0
39	34.54988906399783	35.0	33.0	40.0	27.0	41.0
40	34.39153960227107	35.0	33.0	40.0	25.0	41.0
41	34.43965817249692	35.0	34.0	40.0	26.0	41.0
42	34.38467730034162	35.0	33.0	40.0	26.0	41.0
43	34.2599921687259	35.0	33.0	40.0	26.0	41.0
44	34.22932334324364	35.0	33.0	40.0	26.0	41.0
45	34.156747264234035	35.0	33.0	39.0	26.0	41.0
46	34.11240563462709	35.0	33.0	39.0	25.0	41.0
47	34.13975018573905	35.0	33.0	39.0	26.0	41.0
48	34.13191468308923	35.0	33.0	39.0	26.0	41.0
49	34.11114975535725	35.0	33.0	39.0	26.0	41.0
50	33.89282930993874	35.0	33.0	39.0	24.0	41.0
51	33.709900557196	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	7.0
12	5.0
13	10.0
14	37.0
15	110.0
16	312.0
17	818.0
18	1913.0
19	3949.0
20	7158.0
21	11887.0
22	18426.0
23	26805.0
24	38183.0
25	52728.0
26	70278.0
27	84620.0
28	97272.0
29	113534.0
30	139995.0
31	174243.0
32	222868.0
33	300458.0
34	523463.0
35	778559.0
36	327571.0
37	392479.0
38	543566.0
39	797904.0
40	592.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.15166074176374	24.512522215743143	29.029120753426074	13.306696289067045
2	30.692822501973676	26.57848590011235	29.67257916585091	13.056112432063063
3	29.060264022188047	26.514888512172092	30.922263610327306	13.502583855312558
4	26.37765093068265	28.3409242848571	29.833306341090886	15.44811844336936
5	23.325103166042037	33.269256709757	28.74623077648436	14.659409347716604
6	21.582623536023227	41.57463580558312	26.88645117695339	9.956289481440262
7	87.75600168634563	4.76409555338396	6.009149735905927	1.4707530243644806
8	89.5592244332369	3.600969521882111	5.365522181491891	1.4742838633890896
9	85.35209230754919	5.081765351855508	6.921903337890301	2.644239002705003
10	54.947149471198706	22.20713804565734	12.698102269166641	10.147610213977302
11	49.10741658022806	19.474226355112762	19.10674424082098	12.311612823838196
12	43.70077175261123	22.261305767699547	22.471147548054297	11.566774931634923
13	20.75247465301578	43.85185783446666	21.907418440789943	13.48824907172762
14	13.764246512609324	45.255123205139206	26.78046257796917	14.200167704282293
15	13.214788760683959	23.757028378220095	49.798911317586494	13.229271543509451
16	14.830665611784461	18.267567404139832	47.7036014980906	19.198165485985104
17	15.197809442097835	18.684777263257242	28.8087287414948	37.30868455315012
18	20.270018271563384	22.82141946494469	37.29432862681653	19.614233636675397
19	31.626443996875945	22.510198204811495	25.66175746138171	20.20160033693084
20	33.218894682471856	22.30585353910584	25.881494048104827	18.593757730317474
21	21.48978572669953	31.32680896300315	27.38544118785036	19.79796412244696
22	21.53255750721919	25.22676655064936	23.13023045173174	30.110445490399712
23	18.067366717169644	32.28643603874535	25.317003801889058	24.329193442195937
24	17.350289253944283	23.354893298890385	42.92176717858899	16.373050268576335
25	15.92184286962916	25.990083205173036	39.385198469095855	18.702875456101946
26	15.87503282411728	37.09051252982714	26.800463618192406	20.233991027863183
27	16.233106415259652	38.95583152950449	27.53773240637885	17.273329648857004
28	13.943008452448055	30.499302923576998	38.74400233077661	16.813686293198334
29	14.687486917924272	25.16171031305222	38.50052243731915	21.650280331704355
30	17.895729883626082	34.669688952110405	29.780491754962306	17.654089409301204
31	30.89076091483828	27.670445439657115	24.32037691600874	17.11841672949587
32	33.124090597523676	24.603076608212604	26.49425318948935	15.77857960477437
33	31.72731605068678	26.48399895639393	22.78539222124449	19.003292771674808
34	19.655631138532787	26.292339939878477	26.996562611924425	27.055466309664304
35	21.239053870454995	24.220160287406067	32.76464272771903	21.776143114419906
36	32.620914322393936	26.385579461426534	23.99649114943399	16.997015066745544
37	20.33630078858224	37.05319557845926	26.639715300203775	15.970788332754726
38	19.584887501548707	37.574596902925606	23.015594468549526	19.82492112697616
39	20.78427334698591	35.38285500683545	25.606194317928587	18.22667732825005
40	26.6773916782987	25.52361074170031	25.04836403753768	22.75063354246331
41	16.98003743957931	24.49133718159549	26.87993921036908	31.64868616845612
42	18.931090285033854	25.203805525615074	24.740906186664255	31.12419800268682
43	19.540868298858673	25.04764518408357	27.064705690824514	28.346780826233246
44	17.176665002027587	29.860284488368737	31.768523267806316	21.194527241797353
45	16.23729267949242	40.95270071128435	23.363604111334332	19.446402497888897
46	20.30811750463132	36.25414767871648	25.050034314681056	18.38770050197114
47	20.354166411191787	27.78933111531805	26.59580181125699	25.26070066223317
48	21.160994842437894	24.923727534243852	33.91150998550876	20.003767637809496
49	20.47294637310947	26.123620805648667	34.97860565264071	18.42482716860116
50	18.760996872141764	37.35310546806451	26.117299123802212	17.76859853599151
51	17.74439008878686	36.05229785735157	25.293556493635823	20.909755560225754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9390.0
1	9687.0
2	9984.0
3	18959.0
4	27934.0
5	21276.5
6	14619.0
7	14991.5
8	15364.0
9	16487.5
10	17611.0
11	18888.5
12	20166.0
13	19987.0
14	19808.0
15	19174.0
16	18540.0
17	18376.0
18	18212.0
19	18977.5
20	19743.0
21	19084.5
22	18426.0
23	19924.0
24	21422.0
25	23971.5
26	32164.0
27	37807.0
28	49762.5
29	61718.0
30	78459.0
31	95200.0
32	111072.0
33	126944.0
34	136667.0
35	146390.0
36	151087.5
37	155785.0
38	191858.0
39	227931.0
40	305129.0
41	382327.0
42	462210.0
43	542093.0
44	543145.0
45	544197.0
46	485593.5
47	426990.0
48	390381.5
49	353773.0
50	320099.5
51	286426.0
52	258600.0
53	230774.0
54	201071.0
55	171368.0
56	158071.0
57	144774.0
58	134757.0
59	124740.0
60	119128.5
61	113517.0
62	101215.0
63	88913.0
64	79447.0
65	69981.0
66	57951.5
67	45922.0
68	39392.5
69	32863.0
70	27533.0
71	22203.0
72	19259.0
73	16315.0
74	13712.0
75	8648.0
76	6187.0
77	4498.5
78	2810.0
79	2350.0
80	1890.0
81	1207.5
82	525.0
83	465.0
84	405.0
85	254.0
86	103.0
87	62.0
88	21.0
89	12.5
90	4.0
91	3.5
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4729754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.970929010534896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.74757817037198	10.214292461994424
2	8.909621372553856	2.3113213266828105
3	3.1822504102652895	1.2383023249588987
4	1.5767870555800014	0.8180957185063416
5	0.9748430275664383	0.632230985348959
6	0.6153328539716132	0.47888632600293773
7	0.43700840377314903	0.396788348764408
8	0.326920828188937	0.3392373483603181
9	0.26488546644571204	0.3092229523070778
>10	2.245869651498655	6.713209022533011
>50	0.8770837090567721	8.277672577119427
>100	1.5840864689044258	44.3669507990608
>500	0.2386350200225981	19.715026740915835
>1k	0.018421541913925775	3.3780616033447064
>5k	5.070149150621773E-4	0.487534491417061
>10k+	1.690049716873924E-4	0.3231669726830177
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14742	0.3116864006034986	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8543	0.1806225017199626	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8320	0.17590766877093397	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5377	0.11368455949294615	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.114274865035264E-5	0.0	0.0	0.016618200439177173	0.0
2	2.114274865035264E-5	0.0	0.0	0.06583851929719813	0.0
3	2.114274865035264E-5	0.0	0.0	0.0941486597400203	0.0
4	2.114274865035264E-5	0.0	0.0	0.12801934307788523	0.0
5	2.114274865035264E-5	0.0	0.0	0.23007539081313744	0.0
6	2.114274865035264E-5	0.0	0.0	0.32449890628561234	0.0
7	2.114274865035264E-5	0.0	0.0	0.392049988223489	0.0
8	2.114274865035264E-5	0.0	0.0	0.5539400146392391	0.0
9	2.114274865035264E-5	0.0	0.0	0.6254236478260814	0.0
10	4.228549730070528E-5	0.0	0.0	0.775283450259781	0.0
11	4.228549730070528E-5	0.0	0.0	0.9094553331949188	0.0
12	4.228549730070528E-5	0.0	0.0	1.0431409329110986	0.0
13	1.057137432517632E-4	0.0	0.0	1.0925303937583224	0.0
14	1.057137432517632E-4	0.0	0.0	1.110565158357073	0.0
15	1.057137432517632E-4	0.0	0.0	1.1409895736649305	0.0
16	1.057137432517632E-4	0.0	0.0	1.2116063541571085	0.0
17	1.057137432517632E-4	0.0	0.0	1.2962619197531204	0.0
18	1.057137432517632E-4	0.0	0.0	1.4400326105755183	0.0
19	1.2685649190211583E-4	0.0	0.0	1.4980483128720858	0.0
20	1.2685649190211583E-4	0.0	0.0	1.5627239809935147	0.0
21	1.2685649190211583E-4	0.0	0.0	1.6469989771138203	0.0
22	1.2685649190211583E-4	0.0	0.0	1.7328596793829023	0.0
23	1.2685649190211583E-4	0.0	0.0	1.8382774241535607	0.0
24	1.6914198920282112E-4	0.0	0.0	1.9200364331844744	0.0
25	2.3257023515387904E-4	0.0	0.0	1.9843738173274974	0.0
26	2.3257023515387904E-4	0.0	0.0	2.0498529098976395	0.0
27	2.3257023515387904E-4	0.0	0.0	2.116241140659747	0.0
28	2.3257023515387904E-4	0.0	0.0	2.183052226394861	0.0
29	2.3257023515387904E-4	0.0	0.0	2.2613226818984664	0.0
30	2.3257023515387904E-4	0.0	0.0	2.369721554228825	0.0
31	2.5371298380423167E-4	0.0	0.0	2.4642084979472507	0.0
32	2.5371298380423167E-4	0.0	0.0	2.5477223551161434	0.0
33	2.5371298380423167E-4	0.0	0.0	2.633435058144673	0.0
34	2.5371298380423167E-4	0.0	0.0	2.726949435425183	0.0
35	2.5371298380423167E-4	0.0	0.0	2.861205889354922	0.0
36	2.748557324545843E-4	0.0	0.0	2.960788235498083	0.0
37	2.748557324545843E-4	2.114274865035264E-5	0.0	3.0688065383527348	0.0
38	2.95998481104937E-4	2.114274865035264E-5	0.0	3.169551735671665	0.0
39	2.95998481104937E-4	2.114274865035264E-5	0.0	3.273278060550295	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCGA	60	0.0	45.000004	41
CAATACG	70	0.0	45.000004	12
CGACCGT	20	7.035518E-4	45.0	35
TATCGTG	235	0.0	43.085106	1
CTACGTC	65	0.0	41.53846	41
ACGGGTA	685	0.0	41.38686	5
ACGCTAG	120	0.0	41.250004	1
GCTACGA	710	0.0	40.563377	10
ACGACTT	350	0.0	40.500004	17
TCGACTA	50	1.0822987E-9	40.499996	37
GCGCTAA	235	0.0	40.212765	14
CGAACTA	90	0.0	40.0	24
ACGGGAT	675	0.0	39.666668	5
ACGATAT	120	0.0	39.375004	39
TACGATA	120	0.0	39.375004	38
AATCGTC	40	3.461264E-7	39.375	40
TATGTCG	200	0.0	39.374996	1
CGACGGT	300	0.0	39.0	28
CGCGACC	1165	0.0	38.819744	10
ATTGGGC	935	0.0	38.743317	4
>>END_MODULE