FastQCFastQC Report
Thu 26 May 2016
SRR1545221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545221_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1933303
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79270.41002367450937593No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39650.20508942467890445No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT32520.168209535701336No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20140.10417404824799836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG502.1827873E-1145.021
TCGCTAA253.8917977E-545.010
TTACGCG253.8917977E-545.01
TTACGAG1350.041.6666641
TCTACGG603.6379788E-1241.2500042
TCGATAG501.0822987E-940.51
CGGTCTA1850.040.13513631
GACCGTT451.929402E-840.0000049
GCGATCG451.929402E-840.0000049
TTTACGG1750.039.8571432
TCACGAC2050.039.51219625
ATAGGGC8050.039.1304324
TACGATA356.2505023E-638.57142629
TCTAACG356.2505023E-638.5714261
TCGTAAG1400.038.5714261
AGGGATC13450.038.4758386
AGGGTAC7900.038.4493686
CGTTTTT61700.038.326581
ACCCGCT11550.038.1818234
GCGCGAC11850.038.164569
Produced by FastQC (version 0.11.5)