##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545221_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1933303
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.111710890636388	31.0	31.0	33.0	30.0	34.0
2	31.37514398932811	31.0	31.0	34.0	30.0	34.0
3	31.476504200324523	31.0	31.0	34.0	30.0	34.0
4	35.251076525511	37.0	35.0	37.0	33.0	37.0
5	35.11069449537915	37.0	35.0	37.0	32.0	37.0
6	35.11303091134706	37.0	35.0	37.0	32.0	37.0
7	35.77962947349691	37.0	35.0	37.0	35.0	37.0
8	35.851281459760834	37.0	35.0	37.0	35.0	37.0
9	37.460563088145	39.0	37.0	39.0	35.0	39.0
10	36.66526819644929	39.0	35.0	39.0	32.0	39.0
11	36.362836554849395	38.0	35.0	39.0	32.0	39.0
12	35.61588431818499	37.0	35.0	39.0	31.0	39.0
13	35.282745643078194	37.0	35.0	39.0	30.0	39.0
14	36.08056781580539	38.0	35.0	40.0	30.0	41.0
15	36.454950931126675	38.0	35.0	40.0	31.0	41.0
16	36.34982928180425	38.0	35.0	40.0	31.0	41.0
17	36.419401407849676	38.0	35.0	40.0	31.0	41.0
18	36.472043957931064	38.0	35.0	40.0	31.0	41.0
19	36.40975780826906	38.0	35.0	40.0	31.0	41.0
20	36.2799923240175	38.0	35.0	40.0	31.0	41.0
21	36.16669606367962	38.0	34.0	40.0	30.0	41.0
22	36.065749135029535	38.0	34.0	40.0	30.0	41.0
23	35.97354475734016	37.0	34.0	40.0	30.0	41.0
24	35.79315554778532	37.0	34.0	40.0	30.0	41.0
25	35.73132406042922	37.0	34.0	40.0	30.0	41.0
26	35.586751274890695	37.0	34.0	40.0	29.0	41.0
27	35.55390903546935	37.0	34.0	40.0	29.0	41.0
28	35.55831858741232	37.0	34.0	40.0	29.0	41.0
29	35.5113337123048	37.0	34.0	40.0	29.0	41.0
30	35.42840206630828	36.0	34.0	40.0	29.0	41.0
31	35.33366782133996	37.0	34.0	40.0	29.0	41.0
32	35.18482307222406	37.0	34.0	40.0	28.0	41.0
33	35.02387934017585	36.0	34.0	40.0	27.0	41.0
34	34.855177900204986	36.0	34.0	40.0	26.0	41.0
35	34.6794868678112	36.0	33.0	40.0	26.0	41.0
36	34.48188256057121	36.0	33.0	40.0	24.0	41.0
37	34.3158982321964	36.0	33.0	40.0	24.0	41.0
38	34.35795216787022	36.0	33.0	40.0	24.0	41.0
39	34.339739813159134	36.0	33.0	40.0	24.0	41.0
40	34.174137214911475	35.0	33.0	40.0	23.0	41.0
41	34.133866755495646	35.0	33.0	40.0	23.0	41.0
42	34.05249099597942	35.0	33.0	40.0	23.0	41.0
43	33.91402020273077	35.0	33.0	40.0	23.0	41.0
44	33.85655792185705	35.0	33.0	40.0	23.0	41.0
45	33.80573660724677	35.0	33.0	39.0	23.0	41.0
46	33.7112144345713	35.0	33.0	39.0	23.0	41.0
47	33.73256494196719	35.0	33.0	39.0	23.0	41.0
48	33.697319561393115	35.0	33.0	39.0	23.0	41.0
49	33.647830164231884	35.0	32.0	39.0	24.0	41.0
50	33.435051825813126	35.0	32.0	39.0	23.0	41.0
51	33.26197652411443	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	4.0
13	6.0
14	13.0
15	51.0
16	193.0
17	500.0
18	1117.0
19	2249.0
20	3886.0
21	6237.0
22	9100.0
23	13362.0
24	18458.0
25	25316.0
26	32776.0
27	39375.0
28	45329.0
29	52444.0
30	62685.0
31	77493.0
32	95237.0
33	121434.0
34	187992.0
35	251248.0
36	153177.0
37	183645.0
38	239374.0
39	310437.0
40	161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.985824777595646	24.25713920683928	27.335239225305084	13.421796790259984
2	32.35302484918298	26.153168954892227	28.351065508096767	13.142740687828033
3	30.458236499917497	25.70698954069797	30.39492516175685	13.439848797627688
4	26.797092850939556	28.351582757591544	29.681172583914677	15.170151807554221
5	23.05220650875729	32.365852636653436	29.09993932663426	15.482001527955008
6	21.124262466876637	41.204612003395226	27.16723658940166	10.503888940326478
7	86.36261362031715	4.9116977524992205	6.784399548337741	1.941289078845892
8	87.1851954918603	4.163651533153365	6.733864272698072	1.91728870228826
9	82.16208219818621	5.842022693804334	8.7391888389973	3.2567062690121515
10	48.33086174283079	24.585902985719258	14.800370143738462	12.282865127711487
11	41.28147527831902	23.16481172377015	21.05376136073859	14.499951637172238
12	37.132565355766786	22.21214160429069	25.401967513628232	15.253325526314294
13	21.90887822550319	35.817820589943736	25.80004272480827	16.473258459744798
14	16.77331489166468	38.27822126174738	28.099320178989018	16.849143667598923
15	15.886852707516619	24.041394442567977	43.54283834453265	16.528914505382758
16	18.59491243741928	19.502426676004745	41.82406999833963	20.07859088823635
17	18.49006596482807	20.594133459680144	29.56556732183212	31.35023325365967
18	21.519441080885922	23.981548676022328	34.14265637616039	20.35635386693136
19	30.40666672528828	24.764560961215082	25.587556632354058	19.241215681142585
20	31.139195459790837	23.423901995703726	26.164444993878348	19.272457550627088
21	23.11520749722108	29.330011901910876	27.708331285887418	19.84644931498063
22	22.58011289487473	24.69555987861189	24.859062443910755	27.865264782602623
23	20.537598089901067	30.189008137886304	26.541571600519937	22.731822171692695
24	19.470357207328597	24.56355780754491	38.211806426618075	17.754278558508418
25	19.66913618817123	26.38634502713749	34.10681098617237	19.83770779851891
26	18.205164943105142	33.56483696554549	26.715315705815385	21.51468238553398
27	18.405392222533145	33.89779046533316	28.544775443890586	19.152041868243106
28	17.50424015273343	28.55320661065544	35.98215075443425	17.960402482176878
29	18.220320353302093	26.086081695419704	34.42776429768122	21.26583365359698
30	20.620202834216883	31.529615378448177	29.426944457232	18.423237330102936
31	29.57156741597153	26.834800339108767	25.365242799499093	18.22838944542061
32	29.932193763729742	25.59122910376697	26.44220797257336	18.034369159929923
33	29.504273256701097	26.52682998991881	24.265622098553617	19.70327465482648
34	20.964690997738067	26.751626620348702	27.211771770901922	25.07191061101131
35	21.99148296981901	25.41132972948369	30.595411065932243	22.001776234765064
36	30.070920078228813	27.12859805214185	24.39565862154044	18.404823248088892
37	20.49797677860118	34.71933783788677	26.875714774145592	17.906970609366457
38	20.911983274220336	33.95680863268717	24.717698156988323	20.41350993610417
39	21.774238182012855	32.24166103295759	25.35562195889625	20.628478826133307
40	25.955838272634967	25.749714348966506	26.71609158005755	21.578355798340972
41	19.03591935666577	24.350761365393836	28.24637421035399	28.366945067586407
42	20.39545792873647	25.905509896793212	25.46843407370702	28.230598100763306
43	20.448062202355246	26.003321776255454	27.04832093055253	26.50029509083677
44	18.95802158275242	28.85936658661369	30.84524257190932	21.337369258724575
45	18.806777830479753	35.838096770138975	24.531177989171898	20.823947410209367
46	20.693290187828808	33.633010448957045	25.673575223335398	20.000124139878746
47	20.93225945441558	27.79388435232346	27.20044400696632	24.073412186294647
48	20.904224531798686	27.336790973789416	31.77215366654891	19.98683082786299
49	21.787272869281225	27.62810588924757	31.2753872517655	19.309233989705703
50	19.87562218648603	34.03946510195246	26.700987894810073	19.383924816751435
51	19.81624194448568	33.112243657616006	25.70579986685998	21.365714531038332
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4577.0
1	4382.0
2	4187.0
3	9587.0
4	14987.0
5	11252.0
6	7517.0
7	7514.5
8	7512.0
9	7913.0
10	8314.0
11	8505.0
12	8696.0
13	8803.5
14	8911.0
15	8763.0
16	8615.0
17	8409.0
18	8203.0
19	8154.0
20	8105.0
21	8392.0
22	8679.0
23	9635.0
24	10591.0
25	11558.5
26	16943.5
27	21361.0
28	24250.5
29	27140.0
30	33127.0
31	39114.0
32	43342.5
33	47571.0
34	58566.0
35	69561.0
36	70798.0
37	72035.0
38	80590.0
39	89145.0
40	109244.0
41	129343.0
42	153520.5
43	177698.0
44	174305.5
45	170913.0
46	160831.0
47	150749.0
48	141688.0
49	132627.0
50	124032.5
51	115438.0
52	104017.5
53	92597.0
54	84426.0
55	76255.0
56	74451.5
57	72648.0
58	70892.0
59	69136.0
60	66028.0
61	62920.0
62	57564.0
63	52208.0
64	46647.5
65	41087.0
66	35580.5
67	30074.0
68	27115.5
69	24157.0
70	21509.5
71	18862.0
72	16126.0
73	13390.0
74	10642.0
75	6022.0
76	4150.0
77	3098.5
78	2047.0
79	1510.5
80	974.0
81	683.5
82	393.0
83	326.5
84	260.0
85	169.5
86	79.0
87	58.0
88	37.0
89	22.0
90	7.0
91	9.0
92	11.0
93	5.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1933303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.396228585937283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.67067790647995	15.06518223098794
2	6.496600675806705	2.52019103479003
3	2.7088440552708515	1.5762407549907227
4	1.5329799596020919	1.189361188564123
5	1.0466882199256855	1.0150901985943197
6	0.7266711043070929	0.8456807307561509
7	0.5818831301353107	0.7900436741683623
8	0.5055194595898641	0.7844136794275591
9	0.4115639525446353	0.7184509651158832
>10	6.137443397856109	28.97967179785212
>50	1.269956568696991	17.067617376634267
>100	0.8993773562158573	27.006407547964656
>500	0.008777089167569236	1.0520982038863602
>1k	0.002742840364865386	0.9678282021187633
>5k	2.7428403648653864E-4	0.4217224141488257
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7927	0.41002367450937593	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3965	0.20508942467890445	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3252	0.168209535701336	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2014	0.10417404824799836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016034734338073234	0.0
2	0.0	0.0	0.0	0.04981112634698234	0.0
3	0.0	0.0	0.0	0.07686327492379622	0.0
4	0.0	0.0	0.0	0.1081051444083002	0.0
5	5.17249494776556E-5	0.0	0.0	0.19795138165098797	0.0
6	5.17249494776556E-5	0.0	0.0	0.30657377555406473	0.0
7	5.17249494776556E-5	0.0	0.0	0.3790404297722602	0.0
8	5.17249494776556E-5	0.0	0.0	0.5575949553691274	0.0
9	5.17249494776556E-5	0.0	0.0	0.6304236842336665	0.0
10	5.17249494776556E-5	0.0	0.0	0.7502186672239168	0.0
11	5.17249494776556E-5	0.0	0.0	0.869134326073047	0.0
12	5.17249494776556E-5	0.0	0.0	0.9668427556363385	0.0
13	5.17249494776556E-5	0.0	0.0	1.0099296385512255	0.0
14	5.17249494776556E-5	0.0	0.0	1.0294299445043018	0.0
15	1.034498989553112E-4	0.0	0.0	1.054154470354621	0.0
16	1.034498989553112E-4	0.0	0.0	1.1180865079090034	0.0
17	1.551748484329668E-4	0.0	0.0	1.1896738379860787	0.0
18	1.551748484329668E-4	0.0	0.0	1.3050204753212507	0.0
19	1.551748484329668E-4	0.0	0.0	1.3516246548006183	0.0
20	1.551748484329668E-4	0.0	0.0	1.4058324018532016	0.0
21	1.551748484329668E-4	0.0	0.0	1.47514383415326	0.0
22	1.551748484329668E-4	0.0	0.0	1.5537657573592965	0.0
23	1.551748484329668E-4	0.0	0.0	1.6389567491489954	0.0
24	1.551748484329668E-4	0.0	0.0	1.715147599729582	0.0
25	2.58624747388278E-4	0.0	0.0	1.781510709909414	0.0
26	3.103496968659336E-4	0.0	0.0	1.8445634233226762	0.0
27	3.103496968659336E-4	0.0	0.0	1.9139265805722123	0.0
28	3.103496968659336E-4	0.0	0.0	1.9870656591336175	0.0
29	3.103496968659336E-4	0.0	0.0	2.071222151933763	0.0
30	3.103496968659336E-4	0.0	0.0	2.1752410253333285	0.0
31	3.103496968659336E-4	0.0	0.0	2.2668459108582564	0.0
32	3.103496968659336E-4	0.0	0.0	2.355554199212436	0.0
33	3.103496968659336E-4	0.0	0.0	2.451866055139831	0.0
34	3.103496968659336E-4	0.0	0.0	2.5582125512658904	0.0
35	3.103496968659336E-4	0.0	0.0	2.6890766734443594	0.0
36	3.103496968659336E-4	0.0	0.0	2.7931472717934023	0.0
37	3.103496968659336E-4	0.0	0.0	2.90042481701006	0.0
38	3.103496968659336E-4	0.0	0.0	3.0187197764654585	0.0
39	3.103496968659336E-4	0.0	0.0	3.2035330209491217	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	50	2.1827873E-11	45.0	21
TCGCTAA	25	3.8917977E-5	45.0	10
TTACGCG	25	3.8917977E-5	45.0	1
TTACGAG	135	0.0	41.666664	1
TCTACGG	60	3.6379788E-12	41.250004	2
TCGATAG	50	1.0822987E-9	40.5	1
CGGTCTA	185	0.0	40.135136	31
GACCGTT	45	1.929402E-8	40.000004	9
GCGATCG	45	1.929402E-8	40.000004	9
TTTACGG	175	0.0	39.857143	2
TCACGAC	205	0.0	39.512196	25
ATAGGGC	805	0.0	39.130432	4
TACGATA	35	6.2505023E-6	38.571426	29
TCTAACG	35	6.2505023E-6	38.571426	1
TCGTAAG	140	0.0	38.571426	1
AGGGATC	1345	0.0	38.475838	6
AGGGTAC	790	0.0	38.449368	6
CGTTTTT	6170	0.0	38.32658	1
ACCCGCT	1155	0.0	38.18182	34
GCGCGAC	1185	0.0	38.16456	9
>>END_MODULE