FastQCFastQC Report
Thu 26 May 2016
SRR1545220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545220_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3201584
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134400.4197922028595845No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72250.22566954357592992No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC56860.1775995882038391No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG41460.12949839829284504No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG39810.12434469937380997No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT32060.10013793172379672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC207.035176E-445.00000431
CGTTACA207.035176E-445.00000432
GTCGTAC207.035176E-445.00000441
CTATTCG207.035176E-445.0000049
ACCGTAT253.8923732E-545.04
ATTACGC302.166722E-644.99999631
ACTACGG1300.041.538462
CGTTAGG5550.041.3513532
CGTAAGG3500.041.142862
TACGCGC2850.041.0526334
TATTACG1700.041.0294151
ACGACGG2700.040.83333227
TATACGG3100.040.645162
AGCGGGA12100.040.1652874
GTATGCG1850.040.1351361
CGAACGC451.9301297E-840.00000417
CGTATTA451.9301297E-840.00000435
GTATACG900.040.0000041
GCGTATG1800.040.0000041
CAAATCG850.039.7058831