##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545220_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3201584
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18402047236618	31.0	31.0	33.0	30.0	34.0
2	31.44444093923508	31.0	31.0	34.0	30.0	34.0
3	31.54732220051075	31.0	31.0	34.0	30.0	34.0
4	35.299450209646224	37.0	35.0	37.0	33.0	37.0
5	35.153189171360175	37.0	35.0	37.0	32.0	37.0
6	35.14860800153924	37.0	35.0	37.0	32.0	37.0
7	35.803042493965485	37.0	35.0	37.0	35.0	37.0
8	35.887326398432776	37.0	35.0	37.0	35.0	37.0
9	37.502377885446705	39.0	37.0	39.0	35.0	39.0
10	36.74697774601572	39.0	37.0	39.0	32.0	39.0
11	36.40352369327183	38.0	35.0	39.0	32.0	39.0
12	35.45459903597719	37.0	35.0	39.0	30.0	39.0
13	35.01814882882973	37.0	34.0	39.0	29.0	39.0
14	35.794038825781236	38.0	34.0	40.0	28.0	41.0
15	36.23955360846381	38.0	35.0	40.0	30.0	41.0
16	36.1821545210121	38.0	35.0	40.0	31.0	41.0
17	36.25961586514675	37.0	35.0	40.0	31.0	41.0
18	36.34406343859789	37.0	35.0	40.0	31.0	41.0
19	36.25675353200166	37.0	35.0	40.0	31.0	41.0
20	36.11066990589658	37.0	34.0	40.0	31.0	41.0
21	35.9768920634286	37.0	34.0	40.0	30.0	41.0
22	35.85404537254059	37.0	34.0	40.0	30.0	41.0
23	35.79595100425289	36.0	34.0	40.0	30.0	41.0
24	35.62322150535485	36.0	34.0	40.0	30.0	41.0
25	35.582272087816534	36.0	34.0	40.0	30.0	41.0
26	35.43256837865257	36.0	34.0	40.0	29.0	41.0
27	35.38948970259722	36.0	34.0	40.0	29.0	41.0
28	35.42364623261486	36.0	34.0	40.0	29.0	41.0
29	35.415679238776804	36.0	34.0	40.0	29.0	41.0
30	35.33074877935422	36.0	34.0	40.0	29.0	41.0
31	35.18635275538608	36.0	34.0	40.0	29.0	41.0
32	35.04502146437513	36.0	34.0	40.0	29.0	41.0
33	34.874892240840786	36.0	34.0	40.0	27.0	41.0
34	34.7330465169741	35.0	34.0	40.0	27.0	41.0
35	34.586700520742234	35.0	33.0	40.0	26.0	41.0
36	34.35444642402011	35.0	33.0	40.0	24.0	41.0
37	34.18110878864962	35.0	33.0	40.0	24.0	41.0
38	34.259086127366956	35.0	33.0	40.0	25.0	41.0
39	34.25038918235474	35.0	33.0	40.0	24.0	41.0
40	34.110912285918474	35.0	33.0	40.0	24.0	41.0
41	34.11255803377328	35.0	33.0	40.0	24.0	41.0
42	34.049150982763535	35.0	33.0	40.0	24.0	41.0
43	33.902169676010374	35.0	33.0	40.0	23.0	41.0
44	33.8657242789819	35.0	33.0	39.0	23.0	41.0
45	33.81986666600033	35.0	33.0	39.0	23.0	41.0
46	33.74187464704971	35.0	33.0	39.0	23.0	41.0
47	33.76075967396139	35.0	33.0	39.0	23.0	41.0
48	33.72401348832328	35.0	33.0	39.0	23.0	41.0
49	33.692287942468475	35.0	33.0	39.0	24.0	41.0
50	33.49258523280976	35.0	32.0	39.0	24.0	41.0
51	33.313622256982796	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	7.0
13	16.0
14	39.0
15	119.0
16	307.0
17	856.0
18	1965.0
19	3731.0
20	6380.0
21	10257.0
22	15470.0
23	21854.0
24	30680.0
25	41612.0
26	54063.0
27	65430.0
28	74270.0
29	86621.0
30	103069.0
31	126614.0
32	157410.0
33	204617.0
34	336765.0
35	484130.0
36	229567.0
37	272271.0
38	364002.0
39	509065.0
40	391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.53349467013828	24.241406753656943	27.792680123338947	14.43241845286583
2	30.541631892213356	26.5795931014148	29.65731962678474	13.221455379587105
3	29.594631907205933	26.260750928290495	30.487158856366097	13.657458308137471
4	26.641468722982125	28.85952703411811	29.728003388322783	14.771000854576984
5	22.769853922308457	32.923327952663435	29.078106337363007	15.228711787665105
6	21.12354384579633	41.81626969650023	26.646216372895417	10.413970084808021
7	85.86768299691653	5.5272015352400565	6.591268572056832	2.0138468957865854
8	87.37178221780219	4.2664818414884635	6.367504335354	1.994231605355349
9	82.56987791043433	6.193434250046227	8.10842383020405	3.1282640093153886
10	52.86464450097201	22.636982193814063	13.529646574945403	10.968726730268518
11	46.2205583236298	21.501825346453504	18.94908895096927	13.328527378947422
12	41.60471816450857	22.398631427443416	23.432494665140755	12.56415574290726
13	20.489982458682952	41.75886061399608	23.10412595765096	14.647030969670013
14	14.590402750638434	43.40973093318807	27.141533690823046	14.858332625350451
15	13.690691857530524	23.720196002978525	48.1998598193894	14.38925232010155
16	15.464844901773622	18.71236238062159	46.22889794551697	19.593894772087815
17	15.11451831343485	19.527771253229652	29.204606219921136	36.15310421341436
18	19.800105197927024	23.688805291380767	36.76764376633566	19.743445744356546
19	31.083332500412297	23.560618743721857	25.41660627989145	19.939442475974392
20	32.14146497483746	23.07182944442501	25.942970729488902	18.84373485124863
21	21.766444360041778	30.227349961768923	27.584939205093477	20.421266473095816
22	21.526188286798035	25.390744081679568	23.87433845246603	29.20872917905637
23	18.693777829974163	31.61897360806401	25.7560944832308	23.93115407873103
24	17.839763067281694	24.05696680143329	41.2929662317153	16.81030389956971
25	17.124211015547306	26.441630143079177	37.42516204478783	19.008996796585688
26	16.314112014552794	36.616249956271645	26.3541734341501	20.715464595025463
27	16.983436948710388	37.80225663296668	26.940039680358225	18.274266737964705
28	14.969777460157221	30.384615865146753	37.6666050305099	16.979001644186127
29	15.637603136447458	25.898961264174236	36.741156877345716	21.722278722032595
30	18.37031294509218	34.53787250311096	29.32045512471327	17.771359427083596
31	30.675534360491557	28.09581132339492	23.54803122454385	17.68062309156967
32	31.826714526309473	25.761591762077774	25.439251320596306	16.972442391016447
33	29.43461736440462	26.68054313115008	23.98353440047177	19.90130510397353
34	19.17984972438643	27.1854182179821	26.73892048436024	26.89581157327123
35	20.6638339022184	25.400957775900928	31.039822787720077	22.89538553416059
36	31.298194893527704	26.552387818030077	24.141018945621916	18.008398342820303
37	19.595050450027237	36.28794371785966	26.672953138196593	17.44405269391651
38	19.430381960929342	36.31205678189296	23.57101984517664	20.68654141200106
39	20.260002548738374	34.53012633746296	25.249314089525686	19.960557024272983
40	25.617163254189173	24.931658828879705	26.35632861733442	23.0948492995967
41	16.681992413755193	24.8246805331361	27.074566839414487	31.41876021369422
42	18.789261815401375	25.914672237242563	25.09257917330921	30.203486774046844
43	19.511216947610933	25.566563301165925	27.26138061659479	27.660839134628358
44	17.434401221395408	29.9921226492886	31.040915996581692	21.532560132734297
45	16.504923812712708	39.647062204208915	23.110497803587222	20.737516179491152
46	20.462589768064806	35.84697449762368	24.607725425914172	19.082710308397345
47	20.51953033248542	28.36489687604636	26.3908115482836	24.724761243184624
48	20.99629433430452	25.78989025432411	32.48410786660603	20.72970754476534
49	20.59015162494565	26.728269506594238	33.5375239256568	19.14405494280331
50	19.559568013833154	36.07323749743877	25.4006766650508	18.96651782367728
51	17.9004830109096	34.67446114173484	25.04697674651048	22.378079100845085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8732.0
1	8526.0
2	8320.0
3	16409.5
4	24499.0
5	18203.5
6	11908.0
7	11937.5
8	11967.0
9	12785.0
10	13603.0
11	13811.0
12	14019.0
13	14016.0
14	14013.0
15	13891.0
16	13769.0
17	13309.5
18	12850.0
19	13078.5
20	13307.0
21	15152.5
22	16998.0
23	16967.0
24	16936.0
25	19270.5
26	27233.0
27	32861.0
28	39384.5
29	45908.0
30	51547.0
31	57186.0
32	69044.0
33	80902.0
34	84626.0
35	88350.0
36	93325.5
37	98301.0
38	122927.5
39	147554.0
40	203587.5
41	259621.0
42	300363.0
43	341105.0
44	340460.0
45	339815.0
46	315306.5
47	290798.0
48	258924.5
49	227051.0
50	212588.5
51	198126.0
52	173211.5
53	148297.0
54	132323.5
55	116350.0
56	107937.0
57	99524.0
58	99749.5
59	99975.0
60	93640.0
61	87305.0
62	77563.0
63	67821.0
64	58891.0
65	49961.0
66	44176.0
67	38391.0
68	33234.0
69	28077.0
70	23953.5
71	19830.0
72	17163.5
73	14497.0
74	12392.5
75	7998.5
76	5709.0
77	4137.5
78	2566.0
79	2136.0
80	1706.0
81	1246.0
82	786.0
83	525.0
84	264.0
85	168.5
86	73.0
87	56.5
88	40.0
89	24.5
90	9.0
91	7.0
92	5.0
93	3.0
94	1.0
95	3.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3201584.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.1912591329443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9800678133824	12.787867443039879
2	7.989472309438139	2.5871923299519173
3	2.922240140587671	1.4194424209483965
4	1.4770241300976141	0.9565952174408843
5	1.0061300454971016	0.8145256144042304
6	0.6601844923784381	0.641353091499035
7	0.49435897118353617	0.5603005944989864
8	0.3526708574185751	0.4568148192881441
9	0.29444342388010275	0.4290668798430693
>10	3.125682671544187	12.181430375498316
>50	1.2929112628723731	15.211889640322463
>100	1.3644594521547966	45.917986000238145
>500	0.03316255102873022	3.4918468094459096
>1k	0.006592555324988538	1.691339544237929
>5k	3.9954880757506287E-4	0.4176190949464687
>10k+	1.9977440378753143E-4	0.434730124396293
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13440	0.4197922028595845	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7225	0.22566954357592992	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5686	0.1775995882038391	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4146	0.12949839829284504	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3981	0.12434469937380997	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3206	0.10013793172379672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04176057851363575	0.0
2	0.0	0.0	0.0	0.12428223029600348	0.0
3	0.0	0.0	0.0	0.17422625800228886	0.0
4	0.0	0.0	0.0	0.23394669638528928	0.0
5	3.123453890324289E-5	0.0	0.0	0.4133891223844197	0.0
6	3.123453890324289E-5	0.0	0.0	0.5792757584995427	0.0
7	3.123453890324289E-5	0.0	0.0	0.6983418207987047	0.0
8	6.246907780648578E-5	0.0	0.0	0.9787967456109226	0.0
9	6.246907780648578E-5	0.0	0.0	1.094989230330986	0.0
10	6.246907780648578E-5	0.0	0.0	1.2930786760553525	0.0
11	9.370361670972869E-5	0.0	0.0	1.499007991044433	0.0
12	9.370361670972869E-5	0.0	0.0	1.6872273224753747	0.0
13	1.2493815561297157E-4	0.0	0.0	1.7655010769669013	0.0
14	1.2493815561297157E-4	0.0	0.0	1.7932998165907876	0.0
15	1.2493815561297157E-4	0.0	0.0	1.8407138466459103	0.0
16	1.2493815561297157E-4	0.0	0.0	1.9436628868709989	0.0
17	1.2493815561297157E-4	0.0	0.0	2.0601364824411914	0.0
18	1.2493815561297157E-4	0.0	0.0	2.2532908710188457	0.0
19	1.5617269451621447E-4	0.0	0.0	2.330908700193404	0.0
20	1.5617269451621447E-4	0.0	0.0	2.417428372955387	0.0
21	1.5617269451621447E-4	0.0	0.0	2.5288107386843515	0.0
22	1.5617269451621447E-4	0.0	0.0	2.6497508733177075	0.0
23	1.5617269451621447E-4	0.0	0.0	2.789900249376559	0.0
24	1.8740723341945738E-4	0.0	0.0	2.899408542771328	0.0
25	1.8740723341945738E-4	0.0	0.0	2.986709079005892	0.0
26	2.1864177232270026E-4	0.0	0.0	3.0752589967965855	0.0
27	2.1864177232270026E-4	0.0	0.0	3.15899879559618	0.0
28	2.4987631122594313E-4	0.0	0.0	3.254170435634361	0.0
29	2.4987631122594313E-4	0.0	0.0	3.359087251810354	0.0
30	2.4987631122594313E-4	0.0	0.0	3.5005172439642376	0.0
31	2.4987631122594313E-4	0.0	0.0	3.6229254019260466	0.0
32	2.4987631122594313E-4	0.0	0.0	3.7433033148591446	0.0
33	2.4987631122594313E-4	0.0	0.0	3.861994562691468	0.0
34	2.8111085012918607E-4	0.0	0.0	3.993117157007281	0.0
35	3.1234538903242894E-4	0.0	0.0	4.159784656594986	0.0
36	3.1234538903242894E-4	0.0	0.0	4.286409477308732	0.0
37	3.435799279356718E-4	0.0	0.0	4.421592561681967	0.0
38	3.435799279356718E-4	0.0	0.0	4.5472178771508105	0.0
39	3.435799279356718E-4	0.0	0.0	4.683962688469208	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	20	7.035176E-4	45.000004	31
CGTTACA	20	7.035176E-4	45.000004	32
GTCGTAC	20	7.035176E-4	45.000004	41
CTATTCG	20	7.035176E-4	45.000004	9
ACCGTAT	25	3.8923732E-5	45.0	4
ATTACGC	30	2.166722E-6	44.999996	31
ACTACGG	130	0.0	41.53846	2
CGTTAGG	555	0.0	41.351353	2
CGTAAGG	350	0.0	41.14286	2
TACGCGC	285	0.0	41.05263	34
TATTACG	170	0.0	41.029415	1
ACGACGG	270	0.0	40.833332	27
TATACGG	310	0.0	40.64516	2
AGCGGGA	1210	0.0	40.165287	4
GTATGCG	185	0.0	40.135136	1
CGAACGC	45	1.9301297E-8	40.000004	17
CGTATTA	45	1.9301297E-8	40.000004	35
GTATACG	90	0.0	40.000004	1
GCGTATG	180	0.0	40.000004	1
CAAATCG	85	0.0	39.705883	1
>>END_MODULE