FastQCFastQC Report
Thu 26 May 2016
SRR1545219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545219_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1400074
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67610.4829030465532536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36730.26234327614111824No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15760.11256547868184111No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15750.11249405388572319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG502.1827873E-1145.021
TATTACG351.2121745E-745.01
GCGATAC850.045.09
TCGAACG253.8912433E-545.01
CGGTCTA1100.042.95454431
TCGATAG850.042.352941
ATCGCCA556.184564E-1140.90909224
TCGATTG501.0822987E-940.51
ACGTATG900.040.01
ACCGGGT403.4589357E-739.3754
GACCGAT6750.039.3333329
GGGCGAT22000.039.0681847
AGGGACC7600.038.7828946
TAACGGG2850.038.684213
CGTTGAT3500.038.5714325
TAGGGTA5250.038.571435
TCGTTAG700.038.5714261
CGACCCG4550.038.57142632
TAGCCGT700.038.57142644
TTACGGG3150.038.5714263
Produced by FastQC (version 0.11.5)