##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545219_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1400074
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16308066573624	31.0	31.0	33.0	30.0	34.0
2	31.427855956185173	31.0	31.0	34.0	30.0	34.0
3	31.5161962867677	31.0	31.0	34.0	30.0	34.0
4	35.2861734451179	37.0	35.0	37.0	33.0	37.0
5	35.138945512880035	37.0	35.0	37.0	32.0	37.0
6	35.14306815211196	37.0	35.0	37.0	32.0	37.0
7	35.80262686115162	37.0	35.0	37.0	35.0	37.0
8	35.88803520385351	37.0	35.0	37.0	35.0	37.0
9	37.496108777107494	39.0	37.0	39.0	35.0	39.0
10	36.712231639184786	39.0	37.0	39.0	32.0	39.0
11	36.393202073604684	38.0	35.0	39.0	32.0	39.0
12	35.626851866401346	37.0	35.0	39.0	31.0	39.0
13	35.293583053467174	37.0	35.0	39.0	30.0	39.0
14	36.11897371138954	38.0	35.0	40.0	30.0	41.0
15	36.48270519986801	38.0	35.0	40.0	31.0	41.0
16	36.36769842165486	38.0	35.0	40.0	31.0	41.0
17	36.43257427821672	38.0	35.0	40.0	31.0	41.0
18	36.4757248545434	38.0	35.0	40.0	31.0	41.0
19	36.42082918474309	38.0	35.0	40.0	31.0	41.0
20	36.30967077454478	38.0	35.0	40.0	31.0	41.0
21	36.18308317988906	37.0	34.0	40.0	30.0	41.0
22	36.07022985927887	37.0	34.0	40.0	30.0	41.0
23	35.98578503707661	37.0	34.0	40.0	30.0	41.0
24	35.79500797814973	37.0	34.0	40.0	30.0	41.0
25	35.73360408092715	37.0	34.0	40.0	30.0	41.0
26	35.58876459387147	37.0	34.0	40.0	29.0	41.0
27	35.55057232689129	37.0	34.0	40.0	29.0	41.0
28	35.57587170392422	37.0	34.0	40.0	29.0	41.0
29	35.55287077683037	36.0	34.0	40.0	29.0	41.0
30	35.45443812255638	36.0	34.0	40.0	29.0	41.0
31	35.35922815508323	36.0	34.0	40.0	29.0	41.0
32	35.22583377735748	36.0	34.0	40.0	29.0	41.0
33	35.04751963110521	36.0	34.0	40.0	27.0	41.0
34	34.882929759427	36.0	34.0	40.0	27.0	41.0
35	34.7183598866917	36.0	33.0	40.0	26.0	41.0
36	34.51568345673157	36.0	33.0	40.0	24.0	41.0
37	34.330951792548106	36.0	33.0	40.0	24.0	41.0
38	34.39420059225441	35.0	33.0	40.0	24.0	41.0
39	34.3803263256085	36.0	33.0	40.0	24.0	41.0
40	34.2262437556872	35.0	33.0	40.0	24.0	41.0
41	34.205991968995924	35.0	33.0	40.0	24.0	41.0
42	34.12719113418291	35.0	33.0	40.0	24.0	41.0
43	33.98570147006515	35.0	33.0	40.0	23.0	41.0
44	33.93252999484313	35.0	33.0	40.0	23.0	41.0
45	33.873706675504295	35.0	33.0	40.0	23.0	41.0
46	33.78206437659723	35.0	33.0	39.0	23.0	41.0
47	33.795753653021194	35.0	33.0	39.0	23.0	41.0
48	33.739282352218524	35.0	33.0	39.0	23.0	41.0
49	33.71054458550048	35.0	33.0	39.0	24.0	41.0
50	33.499650018499025	35.0	32.0	39.0	23.0	41.0
51	33.331551046587535	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	4.0
14	16.0
15	47.0
16	130.0
17	421.0
18	829.0
19	1626.0
20	2894.0
21	4516.0
22	6771.0
23	9678.0
24	13350.0
25	18452.0
26	23538.0
27	28423.0
28	32139.0
29	37537.0
30	44670.0
31	53841.0
32	67006.0
33	86283.0
34	137251.0
35	189528.0
36	107095.0
37	126844.0
38	169462.0
39	237553.0
40	166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.69616605979398	24.238075987412095	26.77622754225848	14.289530410535441
2	32.25800921951269	25.66428631629471	28.91768577946594	13.160018684726666
3	29.724000302841137	26.162759968401673	30.538100128993186	13.575139599764013
4	27.17999191471308	28.392356404018642	30.228116513841414	14.199535167426863
5	23.239843036868052	32.86826267754419	29.318664584871946	14.573229700715821
6	21.159952973914237	42.12027364267888	26.849223683890994	9.870549699515884
7	86.33136534211762	5.129228883616152	6.651434138481252	1.8879716357849659
8	87.53123049210257	4.119639390489359	6.558010505159014	1.7911196122490667
9	82.93633050824457	5.918615730311398	8.193852610647722	2.951201150796315
10	49.202685000935666	25.049675945700013	14.04997164435594	11.69766740900838
11	41.48887844499648	22.94321585859033	20.9545352602791	14.61337043613409
12	37.839428487351384	22.073118992281838	25.905059304008216	14.182393216358562
13	21.675997125866203	38.00706248384014	24.633555083517013	15.683385306776643
14	15.72838292833093	40.346367406294235	27.645395886217443	16.27985377915739
15	15.119629391017902	24.38921085599761	45.093045081902815	15.398114671081672
16	17.15423613323296	20.395064832287435	42.70995675942843	19.740742275051176
17	16.924176864937138	20.158363057952652	29.9566308637972	32.96082921331301
18	20.075867418436456	24.38306832353147	35.85646187272958	19.68460238530249
19	29.692787666937605	24.605270864254315	26.204043500557827	19.49789796825025
20	31.296845738153838	22.62537551586559	26.489742685029505	19.588036060951065
21	22.15304333913779	28.963897622554235	28.359500997804403	20.523558040503573
22	22.18704154208992	25.16588408898387	24.79061821018032	27.856456158745896
23	20.17900482403073	30.396964731864173	25.88134627169707	23.54268417240803
24	18.808362986527854	24.3333566654334	39.3513485715755	17.506931776463244
25	18.682726770156435	26.346964517589782	35.20913894551288	19.761169766740903
26	17.830271828489067	34.15690884910369	26.982859477427624	21.029959844979622
27	17.79713072309035	35.15249908219137	27.60025541507092	19.45011477964736
28	16.378277148207882	29.767283729288597	35.49105261579031	18.363386506713216
29	16.834967294585855	26.417675065746526	34.62616975959842	22.121187880069197
30	18.698797349282966	32.67013029311308	30.051911541818505	18.57916081578545
31	28.1542261337615	27.917524359426714	25.572076904506474	18.356172602305307
32	29.69700172990856	25.71814061256762	26.693446203557812	17.891411453966004
33	28.331002504153354	27.59782697200291	24.358855317647496	19.712315206196244
34	19.942303049695944	27.1904199349463	27.192134130053127	25.675142885304638
35	20.9469642318906	26.11019131845888	30.989861964439026	21.952982485211496
36	29.450657608097856	27.90873910950421	24.510061611029133	18.1305416713688
37	20.184718807720163	35.40034312472055	27.18692011993652	17.228017947622767
38	19.91437595441384	34.45196468186681	25.201810761431183	20.431848602288163
39	20.69833451660412	33.70350424334714	25.74256789283995	19.855593347208792
40	24.99996428760194	25.726997287286242	27.699464456878708	21.57357396823311
41	17.685065217981336	26.45053047196077	27.72646302981128	28.137941280246615
42	19.613034739592337	27.010215174340786	25.826849152259097	27.549900933807788
43	20.293141648227163	25.789708258277777	27.628468209537495	26.28868188395756
44	18.211608814962638	29.847279500940665	30.17047670337425	21.77063498072245
45	17.744133524370856	37.80664450593326	23.479401803047555	20.969820166648333
46	20.423920449919073	34.5866004225491	25.39244354226991	19.59703558526192
47	20.69383475444869	28.712482340219157	26.92114845358174	23.67253445175041
48	20.85261207621883	26.699803010412303	32.08537548729567	20.362209426073193
49	21.24809117232375	26.972788581174996	32.12430200117994	19.65481824532132
50	19.208698968768793	35.044076241684365	26.537597298428512	19.20962749111833
51	18.636014953495312	33.51422853363465	26.58530906223528	21.26444745063475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4317.0
1	4160.0
2	4003.0
3	8179.5
4	12356.0
5	9093.5
6	5831.0
7	5861.5
8	5892.0
9	6124.5
10	6357.0
11	6714.0
12	7071.0
13	7079.5
14	7088.0
15	6943.5
16	6799.0
17	6226.0
18	5653.0
19	5291.0
20	4929.0
21	5320.5
22	5712.0
23	6277.5
24	6843.0
25	8522.0
26	11872.5
27	13544.0
28	17292.5
29	21041.0
30	25351.0
31	29661.0
32	33860.5
33	38060.0
34	41365.0
35	44670.0
36	47582.5
37	50495.0
38	57496.0
39	64497.0
40	80898.0
41	97299.0
42	116014.0
43	134729.0
44	135684.0
45	136639.0
46	128867.5
47	121096.0
48	110449.5
49	99803.0
50	92138.5
51	84474.0
52	74112.0
53	63750.0
54	58225.0
55	52700.0
56	50316.0
57	47932.0
58	47625.5
59	47319.0
60	44713.0
61	42107.0
62	37539.0
63	32971.0
64	29438.0
65	25905.0
66	22158.0
67	18411.0
68	15750.5
69	13090.0
70	11468.5
71	9847.0
72	8776.0
73	7705.0
74	6087.0
75	3405.0
76	2341.0
77	1792.5
78	1244.0
79	956.5
80	669.0
81	495.0
82	321.0
83	228.0
84	135.0
85	105.5
86	76.0
87	42.5
88	9.0
89	8.0
90	7.0
91	4.0
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1400074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.76508163332681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.72866825770822	15.932770599877626
2	6.794105776824244	2.8216032216242537
3	2.641353639159161	1.6454377181887445
4	1.537768625839061	1.2772756419486755
5	1.0725279215665329	1.1135564922675694
6	0.7857918670668481	0.9790219359868432
7	0.6558218717318913	0.9532736292403733
8	0.5419187542062023	0.9002389735778044
9	0.47703864071722923	0.8915171685070069
>10	6.768712531272501	33.08822531341657
>50	1.3211747338269135	19.503079527806776
>100	0.6680295335712176	19.062716218644347
>500	0.004607100231525263	0.5742952338260163
>1k	0.0021263539530116597	0.7594473107489094
>5k	3.543923255019433E-4	0.4975410143385055
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6761	0.4829030465532536	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3673	0.26234327614111824	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1576	0.11256547868184111	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1575	0.11249405388572319	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.142479611791949E-5	0.0	0.0	0.025141528233507657	0.0
2	7.142479611791949E-5	0.0	0.0	0.08235278992396117	0.0
3	7.142479611791949E-5	0.0	0.0	0.1235648972840007	0.0
4	7.142479611791949E-5	0.0	0.0	0.16334850872168186	0.0
5	7.142479611791949E-5	0.0	0.0	0.28662770682121086	0.0
6	7.142479611791949E-5	0.0	0.0	0.41776363249371107	0.0
7	7.142479611791949E-5	0.0	0.0	0.5084016987673509	0.0
8	7.142479611791949E-5	0.0	0.0	0.7203904936453359	0.0
9	7.142479611791949E-5	0.0	0.0	0.8168853932006451	0.0
10	7.142479611791949E-5	0.0	0.0	0.9737342454755963	0.0
11	7.142479611791949E-5	0.0	0.0	1.130083124177722	0.0
12	7.142479611791949E-5	0.0	0.0	1.2669330335396558	0.0
13	7.142479611791949E-5	0.0	0.0	1.327929809424359	0.0
14	7.142479611791949E-5	0.0	0.0	1.3531427624539847	0.0
15	7.142479611791949E-5	0.0	0.0	1.3910693291925997	0.0
16	7.142479611791949E-5	0.0	0.0	1.4717793488058488	0.0
17	7.142479611791949E-5	0.0	0.0	1.568274248361158	0.0
18	7.142479611791949E-5	0.0	0.0	1.714695080402893	0.0
19	7.142479611791949E-5	0.0	0.0	1.7771917770060726	0.0
20	1.4284959223583897E-4	0.0	0.0	1.8496879450657608	0.0
21	1.4284959223583897E-4	0.0	0.0	1.9413973832811695	0.0
22	1.4284959223583897E-4	0.0	0.0	2.0362495125257665	0.0
23	1.4284959223583897E-4	0.0	0.0	2.1544575501009233	0.0
24	1.4284959223583897E-4	0.0	0.0	2.248881130568813	0.0
25	1.4284959223583897E-4	0.0	0.0	2.318877430764374	0.0
26	1.4284959223583897E-4	0.0	0.0	2.3926592451541846	0.0
27	1.4284959223583897E-4	0.0	0.0	2.467798130670236	0.0
28	1.4284959223583897E-4	0.0	0.0	2.546722530380537	0.0
29	1.4284959223583897E-4	0.0	0.0	2.6394319157415964	0.0
30	1.4284959223583897E-4	0.0	0.0	2.7571399797439278	0.0
31	1.4284959223583897E-4	0.0	0.0	2.8596345621731425	0.0
32	1.4284959223583897E-4	0.0	0.0	2.9559866121362157	0.0
33	1.4284959223583897E-4	0.0	0.0	3.053267184448822	0.0
34	1.4284959223583897E-4	0.0	0.0	3.1607615026062907	0.0
35	1.4284959223583897E-4	0.0	0.0	3.3024682981042432	0.0
36	1.4284959223583897E-4	0.0	0.0	3.416105148727853	0.0
37	1.4284959223583897E-4	0.0	0.0	3.5336703631379485	0.0
38	1.4284959223583897E-4	0.0	0.0	3.6380934150623467	0.0
39	1.4284959223583897E-4	0.0	0.0	3.7604440908123427	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	50	2.1827873E-11	45.0	21
TATTACG	35	1.2121745E-7	45.0	1
GCGATAC	85	0.0	45.0	9
TCGAACG	25	3.8912433E-5	45.0	1
CGGTCTA	110	0.0	42.954544	31
TCGATAG	85	0.0	42.35294	1
ATCGCCA	55	6.184564E-11	40.909092	24
TCGATTG	50	1.0822987E-9	40.5	1
ACGTATG	90	0.0	40.0	1
ACCGGGT	40	3.4589357E-7	39.375	4
GACCGAT	675	0.0	39.333332	9
GGGCGAT	2200	0.0	39.068184	7
AGGGACC	760	0.0	38.782894	6
TAACGGG	285	0.0	38.68421	3
CGTTGAT	350	0.0	38.57143	25
TAGGGTA	525	0.0	38.57143	5
TCGTTAG	70	0.0	38.571426	1
CGACCCG	455	0.0	38.571426	32
TAGCCGT	70	0.0	38.571426	44
TTACGGG	315	0.0	38.571426	3
>>END_MODULE