FastQCFastQC Report
Thu 26 May 2016
SRR1545218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545218_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences787816
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.40796328076606725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28080.35642840460208985No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18510.23495333935842888No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18040.22898747930988964No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9860.1251561278268022No Hit
GTGTATGGGAAGATAATTTTTTGTCTAATTGTTTTATGTTTATGTCTTTCT8080.1025620195578663No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCCA302.1646283E-645.0000049
TCACATG302.1646283E-645.00000423
GCGCGAC302.1646283E-645.0000049
CGTTGAA302.1646283E-645.00000417
TCTAGCG302.1646283E-645.0000041
ACGCTAG302.1646283E-645.0000041
TCGTAAG302.1646283E-645.0000041
ATCGAAG302.1646283E-645.0000041
GCATAGC302.1646283E-645.0000049
GTTGAGC253.8896847E-545.013
CGGAATA207.0319336E-445.045
TAATACG253.8896847E-545.01
CGGAAAG207.0319336E-445.015
CATATAG207.0319336E-445.01
TCCGATA253.8896847E-545.014
CCTAGGT207.0319336E-445.037
GGCGTAA207.0319336E-445.022
TTCACGA253.8896847E-545.039
ACCTGTT351.2112832E-745.043
CGACCCG253.8896847E-545.032