##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545218_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	787816
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.313050255389584	31.0	31.0	34.0	30.0	34.0
2	31.552622693623892	31.0	31.0	34.0	30.0	34.0
3	31.637582887374716	31.0	31.0	34.0	30.0	34.0
4	35.37092798318389	37.0	35.0	37.0	33.0	37.0
5	35.22271063293967	37.0	35.0	37.0	32.0	37.0
6	35.21864369345126	37.0	35.0	37.0	32.0	37.0
7	35.87376113204098	37.0	35.0	37.0	35.0	37.0
8	36.000038079957754	37.0	35.0	37.0	35.0	37.0
9	37.709952577759275	39.0	38.0	39.0	35.0	39.0
10	36.93516125592778	39.0	37.0	39.0	32.0	39.0
11	36.47720533728688	39.0	35.0	39.0	32.0	39.0
12	35.02468342861785	35.0	35.0	39.0	30.0	39.0
13	34.40982031337266	35.0	33.0	39.0	26.0	39.0
14	35.02080815825015	35.0	33.0	40.0	25.0	41.0
15	35.625382068909495	35.0	33.0	40.0	30.0	41.0
16	35.69784949785229	35.0	34.0	40.0	31.0	41.0
17	35.81347548158453	35.0	34.0	40.0	31.0	41.0
18	35.923279039775785	36.0	35.0	40.0	31.0	41.0
19	35.773557531200176	36.0	35.0	40.0	31.0	41.0
20	35.58377590706459	35.0	34.0	40.0	30.0	41.0
21	35.465952709769795	35.0	34.0	40.0	30.0	41.0
22	35.32238492236766	35.0	34.0	40.0	30.0	41.0
23	35.3411139149243	35.0	34.0	40.0	30.0	41.0
24	35.157364156097366	35.0	34.0	40.0	30.0	41.0
25	35.15363866689684	35.0	34.0	39.0	30.0	41.0
26	35.01571052123846	35.0	34.0	39.0	29.0	41.0
27	34.9522388476497	35.0	34.0	39.0	29.0	41.0
28	35.09809524051301	35.0	34.0	39.0	29.0	41.0
29	35.18937670725144	36.0	34.0	39.0	30.0	41.0
30	35.12763894107253	36.0	34.0	39.0	30.0	41.0
31	34.89979259116342	35.0	34.0	39.0	29.0	41.0
32	34.687526021304464	35.0	34.0	39.0	29.0	41.0
33	34.49784340505905	35.0	33.0	39.0	27.0	41.0
34	34.385498644353504	35.0	34.0	39.0	27.0	41.0
35	34.24466118992252	35.0	33.0	39.0	27.0	41.0
36	33.93299577566335	35.0	33.0	39.0	24.0	41.0
37	33.70689094915564	35.0	33.0	39.0	23.0	41.0
38	33.87695984849254	35.0	33.0	39.0	25.0	41.0
39	33.91514516079897	35.0	33.0	39.0	25.0	41.0
40	33.71772215847355	35.0	33.0	39.0	23.0	41.0
41	33.856816820171204	35.0	33.0	39.0	24.0	41.0
42	33.828741736649164	35.0	33.0	39.0	24.0	41.0
43	33.69146095027265	35.0	33.0	39.0	24.0	41.0
44	33.68373198817998	35.0	33.0	38.0	24.0	41.0
45	33.606719081612965	35.0	33.0	38.0	24.0	41.0
46	33.5770522558567	35.0	33.0	38.0	23.0	41.0
47	33.612546584481656	35.0	33.0	38.0	24.0	41.0
48	33.66295810189181	35.0	33.0	38.0	24.0	41.0
49	33.70058617748307	35.0	33.0	38.0	24.0	40.0
50	33.46479127105822	35.0	33.0	38.0	24.0	40.0
51	33.27351945124242	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	4.0
15	8.0
16	62.0
17	177.0
18	417.0
19	832.0
20	1518.0
21	2465.0
22	3667.0
23	5517.0
24	7534.0
25	10558.0
26	13606.0
27	16302.0
28	18230.0
29	20877.0
30	25759.0
31	31939.0
32	40644.0
33	54617.0
34	98774.0
35	156201.0
36	46117.0
37	51484.0
38	70601.0
39	109797.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.368004711760104	24.43045076515328	30.84159245306011	14.359952070026505
2	28.932263371142504	26.659905358611656	31.14547051595804	13.262360754287803
3	27.108994993754887	26.04351777572428	33.38076403627243	13.466723194248404
4	24.881063598606783	28.913350325456705	31.575900971800525	14.629685104135993
5	21.660641571128284	33.06178092346436	30.915467571107975	14.36210993429938
6	19.677310437970288	39.691755435279305	28.870954639154323	11.759979487596087
7	87.14687185840349	5.103349005351503	6.164764361221402	1.5850147750236097
8	90.12028189323395	3.3252688445017617	5.261761629619099	1.2926876326451862
9	87.06817327903977	4.166582044538319	6.588086558282645	2.177158118139261
10	63.392848076200536	18.54925515602628	10.671400428526457	7.386496339246727
11	58.499573504473126	15.737811874854026	16.645384201387124	9.117230419285722
12	52.155579475410505	19.971795444621588	19.01928876793566	8.853336312032251
13	18.879027590198728	53.67446205713009	17.62416604892513	9.822344303746053
14	11.043187749423723	53.2124252363496	24.463707261594077	11.280679752632594
15	8.977857773896442	22.262685703260658	57.7233770322004	11.036079490642486
16	10.254044091513755	14.689978370583995	55.65081186469937	19.40516567320288
17	10.499405952658996	16.107822131055983	29.39671192258091	43.996059993704115
18	18.327756735075194	21.088680605623647	41.31865816383521	19.264904495465945
19	32.5413548341237	22.211404693481725	25.190526721975694	20.05671375041888
20	35.85723062237883	21.023690811052326	25.014978116717607	18.104100449851234
21	18.97232348670248	33.530418270256	27.325543020197607	20.171715222843915
22	20.008859936838043	25.761218355555105	20.74126445769063	33.48865724991622
23	15.434568477918702	34.62648638768444	23.25999979690689	26.678945337489974
24	14.689597571006427	21.447520740883657	49.94173766463235	13.921144023477563
25	11.213785960173443	25.86403424149801	44.12553185007667	18.796647948251877
26	12.200691532032861	41.131304771672575	27.62980188267311	19.038201813621455
27	12.215923515135515	45.042497232856405	26.38179473379571	16.359784518212376
28	9.72244788123115	31.8461417386801	43.592920174253884	14.838490205834864
29	10.195781756146104	23.510058186175453	42.80580744742427	23.48835261025417
30	14.715238075895895	38.16804939224387	31.577170303725744	15.539542228134486
31	33.374544309838846	29.434284147567453	22.75734435451933	14.433827188074371
32	36.532515206596464	23.472612894381427	25.427130192836906	14.567741706185199
33	34.56860485189435	27.27375427764859	20.78822973892381	17.36941113153325
34	17.80859997765976	25.762487687480323	25.714506940706965	30.714405394152948
35	18.374087350345768	24.586070859185394	34.49358733511379	22.546254455355058
36	35.95243051677041	24.925109416411953	24.18432730485291	14.938132761964724
37	18.103465783888623	40.64705464220072	27.417696517968665	13.831783055941996
38	16.40687673263808	40.91704154269525	23.070234674086336	19.605847050580337
39	18.569437533637295	39.75915696050855	25.054327406399462	16.617078099454694
40	26.81438305391107	24.154624937802733	24.53542451536907	24.49556749291713
41	13.902357910984293	22.96005666297714	26.424825086060704	36.71276033997786
42	17.374488459234136	22.81878001970003	23.743869126801183	36.06286239426465
43	17.837794611939845	23.09143251723753	27.42188531332189	31.64888755750074
44	14.416564273891366	30.492018440854206	32.903368299196764	22.188048986057655
45	12.347299369395898	48.35900768707414	20.595545152675243	18.698147790854716
46	18.5407506321273	40.77360703514527	23.824090904475156	16.861551428252284
47	17.934263838256648	29.05310377042355	26.115615829076837	26.89701656224296
48	19.781014856260853	22.752267026818444	37.53465276155853	19.932065355362166
49	19.806528427957797	24.37561562598373	37.55699300344243	18.260862942616043
50	17.241335540278442	40.91577221077003	25.237745869593915	16.605146379357617
51	15.034221188703961	39.18630746265626	24.176457446916537	21.603013901723244
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3175.0
1	2691.5
2	2208.0
3	3846.0
4	5484.0
5	3927.0
6	2370.0
7	2510.5
8	2651.0
9	2968.5
10	3286.0
11	3575.5
12	3865.0
13	3776.5
14	3688.0
15	3426.0
16	3164.0
17	2886.5
18	2609.0
19	2492.0
20	2375.0
21	2568.0
22	2761.0
23	2653.5
24	2546.0
25	3206.0
26	4679.0
27	5492.0
28	6919.5
29	8347.0
30	9483.5
31	10620.0
32	13652.0
33	16684.0
34	20236.5
35	23789.0
36	25595.0
37	27401.0
38	33420.5
39	39440.0
40	57897.0
41	76354.0
42	93062.5
43	109771.0
44	108650.0
45	107529.0
46	97779.5
47	88030.0
48	74622.0
49	61214.0
50	52809.5
51	44405.0
52	39152.5
53	33900.0
54	28637.0
55	23374.0
56	19734.5
57	16095.0
58	14652.5
59	13210.0
60	12750.5
61	12291.0
62	10653.5
63	9016.0
64	7891.0
65	6766.0
66	5292.5
67	3819.0
68	3194.0
69	2569.0
70	2052.5
71	1536.0
72	1193.0
73	850.0
74	711.0
75	449.0
76	326.0
77	257.5
78	189.0
79	163.5
80	138.0
81	83.5
82	29.0
83	18.0
84	7.0
85	5.0
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	787816.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.406532375619317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.77377835847433	16.27906190797686
2	7.429515194753411	3.032212847137823
3	2.372463700508043	1.4524127194319698
4	1.1444838995688196	0.9341979099970467
5	0.7168410441767685	0.7314119988082991
6	0.5244720839701411	0.6421593936987131
7	0.3874566960053216	0.5534653327828161
8	0.3709537432160443	0.6055903656636307
9	0.30751237301083034	0.564773507617413
>10	4.654069586002647	23.488533037855817
>50	1.2349518638687391	18.07138011949867
>100	1.0778220661664828	31.958504433398765
>500	0.0031552168213304526	0.44014976590453103
>1k	0.0025241734570643623	1.2461466602276614
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3214	0.40796328076606725	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2808	0.35642840460208985	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1851	0.23495333935842888	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1804	0.22898747930988964	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	986	0.1251561278268022	No Hit
GTGTATGGGAAGATAATTTTTTGTCTAATTGTTTTATGTTTATGTCTTTCT	808	0.1025620195578663	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05686607024990607	0.0
2	0.0	0.0	0.0	0.1693288788244971	0.0
3	0.0	0.0	0.0	0.23571493851356154	0.0
4	0.0	0.0	0.0	0.31415965149222663	0.0
5	0.0	0.0	0.0	0.5260111498116311	0.0
6	0.0	0.0	0.0	0.7231383977984707	0.0
7	0.0	0.0	0.0	0.8746966296698722	0.0
8	0.0	0.0	0.0	1.2614620672847467	0.0
9	0.0	0.0	0.0	1.4257136184083594	0.0
10	0.0	0.0	0.0	1.6937965210150594	0.0
11	0.0	0.0	0.0	1.9392853153528236	0.0
12	0.0	0.0	0.0	2.1597682707637316	0.0
13	1.2693319252211175E-4	0.0	0.0	2.2501447038394753	0.0
14	1.2693319252211175E-4	0.0	0.0	2.2811164028148703	0.0
15	1.2693319252211175E-4	0.0	0.0	2.319196360571504	0.0
16	1.2693319252211175E-4	0.0	0.0	2.4291205052956526	0.0
17	1.2693319252211175E-4	0.0	0.0	2.560115559978472	0.0
18	1.2693319252211175E-4	0.0	0.0	2.778948383886593	0.0
19	1.2693319252211175E-4	0.0	0.0	2.8599317607157	0.0
20	1.2693319252211175E-4	0.0	0.0	2.9532276572194522	0.0
21	1.2693319252211175E-4	0.0	0.0	3.08054164931913	0.0
22	1.2693319252211175E-4	0.0	0.0	3.201762848177747	0.0
23	1.2693319252211175E-4	0.0	0.0	3.358271474557511	0.0
24	1.2693319252211175E-4	0.0	0.0	3.473146013790022	0.0
25	1.2693319252211175E-4	0.0	0.0	3.5621261817480225	0.0
26	1.2693319252211175E-4	0.0	0.0	3.653645013556465	0.0
27	1.2693319252211175E-4	0.0	0.0	3.740213450856545	0.0
28	1.2693319252211175E-4	0.0	0.0	3.8291936188145455	0.0
29	1.2693319252211175E-4	0.0	0.0	3.922997248088386	0.0
30	1.2693319252211175E-4	0.0	0.0	4.071889882916823	0.0
31	1.2693319252211175E-4	0.0	0.0	4.206692933375306	0.0
32	1.2693319252211175E-4	0.0	0.0	4.30608162312012	0.0
33	1.2693319252211175E-4	0.0	0.0	4.411182306528428	0.0
34	1.2693319252211175E-4	0.0	0.0	4.526437645338506	0.0
35	1.2693319252211175E-4	0.0	0.0	4.717091300506717	0.0
36	1.2693319252211175E-4	0.0	0.0	4.851513551387634	0.0
37	1.2693319252211175E-4	0.0	0.0	4.971084618743463	0.0
38	1.2693319252211175E-4	0.0	0.0	5.090782619291814	0.0
39	1.2693319252211175E-4	0.0	0.0	5.205657158524326	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCCA	30	2.1646283E-6	45.000004	9
TCACATG	30	2.1646283E-6	45.000004	23
GCGCGAC	30	2.1646283E-6	45.000004	9
CGTTGAA	30	2.1646283E-6	45.000004	17
TCTAGCG	30	2.1646283E-6	45.000004	1
ACGCTAG	30	2.1646283E-6	45.000004	1
TCGTAAG	30	2.1646283E-6	45.000004	1
ATCGAAG	30	2.1646283E-6	45.000004	1
GCATAGC	30	2.1646283E-6	45.000004	9
GTTGAGC	25	3.8896847E-5	45.0	13
CGGAATA	20	7.0319336E-4	45.0	45
TAATACG	25	3.8896847E-5	45.0	1
CGGAAAG	20	7.0319336E-4	45.0	15
CATATAG	20	7.0319336E-4	45.0	1
TCCGATA	25	3.8896847E-5	45.0	14
CCTAGGT	20	7.0319336E-4	45.0	37
GGCGTAA	20	7.0319336E-4	45.0	22
TTCACGA	25	3.8896847E-5	45.0	39
ACCTGTT	35	1.2112832E-7	45.0	43
CGACCCG	25	3.8896847E-5	45.0	32
>>END_MODULE