##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545217_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2523290
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.193443480535333	31.0	31.0	33.0	30.0	34.0
2	31.473803248932942	31.0	31.0	34.0	30.0	34.0
3	31.532574931934104	31.0	31.0	34.0	30.0	34.0
4	35.31773200860781	37.0	35.0	37.0	33.0	37.0
5	35.1804275370647	37.0	35.0	37.0	32.0	37.0
6	35.17721823492345	37.0	35.0	37.0	32.0	37.0
7	35.85877009776918	37.0	35.0	37.0	35.0	37.0
8	35.958836677512295	37.0	35.0	37.0	35.0	37.0
9	37.60597196517245	39.0	37.0	39.0	35.0	39.0
10	36.77741797415279	39.0	37.0	39.0	32.0	39.0
11	36.41572946431048	39.0	35.0	39.0	32.0	39.0
12	36.0809534377737	38.0	35.0	39.0	31.0	39.0
13	35.91381648561996	38.0	35.0	39.0	31.0	39.0
14	36.806195879189474	39.0	35.0	41.0	31.0	41.0
15	37.086948388809844	39.0	35.0	41.0	32.0	41.0
16	36.87012234027797	39.0	35.0	41.0	31.0	41.0
17	36.90690725203999	38.0	35.0	41.0	31.0	41.0
18	36.88555932928835	38.0	35.0	40.0	31.0	41.0
19	36.80920940518133	38.0	35.0	40.0	31.0	41.0
20	36.67236583983609	38.0	35.0	40.0	31.0	41.0
21	36.59985534758193	38.0	35.0	40.0	31.0	41.0
22	36.50701385889057	38.0	35.0	40.0	31.0	41.0
23	36.3669443464683	38.0	35.0	40.0	30.0	41.0
24	36.200749022110024	38.0	35.0	40.0	30.0	41.0
25	36.205274463101745	38.0	35.0	40.0	30.0	41.0
26	36.07312912903392	38.0	35.0	40.0	30.0	41.0
27	36.03244454660384	38.0	34.0	40.0	30.0	41.0
28	36.018702170578884	38.0	35.0	40.0	30.0	41.0
29	35.99798675538682	38.0	35.0	40.0	30.0	41.0
30	35.875185174910534	38.0	35.0	40.0	30.0	41.0
31	35.74761521664176	38.0	34.0	40.0	29.0	41.0
32	35.52532566609466	38.0	34.0	40.0	28.0	41.0
33	35.27305264159094	38.0	34.0	40.0	27.0	41.0
34	35.02922533676272	38.0	34.0	40.0	25.0	41.0
35	34.806374217787095	38.0	34.0	40.0	24.0	41.0
36	34.660867755985244	38.0	33.0	40.0	24.0	41.0
37	34.49810683670922	37.0	33.0	40.0	23.0	41.0
38	34.499342921344756	37.0	33.0	40.0	23.0	41.0
39	34.46581407606735	37.0	33.0	40.0	23.0	41.0
40	34.39116510587368	37.0	33.0	40.0	23.0	41.0
41	34.35573041544967	37.0	33.0	40.0	23.0	41.0
42	34.266446979934926	37.0	33.0	40.0	23.0	41.0
43	34.13850726630708	37.0	33.0	40.0	23.0	41.0
44	34.108014536577244	37.0	33.0	40.0	23.0	41.0
45	34.048228305109596	37.0	33.0	40.0	23.0	41.0
46	33.94681744864839	37.0	33.0	40.0	23.0	41.0
47	33.94098419127409	37.0	33.0	40.0	23.0	41.0
48	33.9001276111743	36.0	33.0	40.0	23.0	41.0
49	33.90099235521878	36.0	33.0	40.0	23.0	41.0
50	33.727998367211065	36.0	33.0	40.0	22.0	41.0
51	33.58118012594668	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	3.0
13	6.0
14	17.0
15	61.0
16	228.0
17	637.0
18	1521.0
19	2897.0
20	5036.0
21	8328.0
22	12686.0
23	18930.0
24	26359.0
25	36827.0
26	47406.0
27	52847.0
28	56577.0
29	62048.0
30	72153.0
31	87557.0
32	108013.0
33	136167.0
34	200312.0
35	270856.0
36	200877.0
37	247780.0
38	351445.0
39	515507.0
40	203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.23157068747548	23.6210661477674	26.611764799131294	17.53559836562583
2	34.0841520395991	25.180656999393648	28.614705404452124	12.120485556555133
3	27.457604952264703	25.903126473770357	34.071866491762734	12.567402082202204
4	24.35764418675618	28.202624351541044	33.66232181001788	13.777409651684902
5	22.341348002013245	32.3475304067309	31.428254382175652	13.882867209080207
6	20.344074601016928	41.037256914583736	28.844682933788825	9.773985550610513
7	85.91212266525052	3.4197020556495685	9.347003317097915	1.3211719620019895
8	87.31869900011493	3.151401543223331	8.351873942353038	1.1780255143087002
9	82.46372791078315	5.339774659274202	9.579953156395025	2.61654427354763
10	39.961716647709935	36.65044445941608	14.684677544000094	8.703161348873891
11	29.0437088087378	23.826591473829804	32.37860095351704	14.751098763915365
12	27.779367413178825	23.843513825204397	32.78743228087141	15.589686480745376
13	20.493522345826282	31.532919323581517	32.84335133892656	15.130206991665643
14	15.957103622651381	35.60347799896167	33.21869464072699	15.220723737659961
15	15.766162430794717	26.757526879589744	42.25764775352813	15.21866293608741
16	19.1416365142334	24.0599772519211	39.60103674171419	17.197349492131305
17	18.690756908639116	24.263481407210428	33.07475557704426	23.9710061071062
18	20.053501579287357	25.4405557823318	36.09799111477476	18.407951523606087
19	25.080787384723912	25.725699384533684	31.470540445212404	17.722972785530004
20	26.27014730768164	26.60114374487276	30.49475090060992	16.63395804683568
21	22.108358531916664	28.36479358298095	32.55158939321283	16.97525849188956
22	20.76911492535539	25.095807457723847	32.16479279036496	21.970284826555805
23	18.176983224282584	29.47905314093901	32.25530953635928	20.088654098419127
24	17.40010066223066	27.21371701231329	38.81975516092086	16.56642716453519
25	17.878008473064927	28.646647828826648	35.8399549794118	17.635388718696625
26	17.913993238985608	32.364928327699154	31.625259086351548	18.095819346963687
27	17.856845626146818	31.95859374071153	32.8535364543909	17.33102417875076
28	16.22508708868184	29.10846553507524	37.460656523824056	17.205790852418865
29	17.126212207078854	26.44404725576529	37.62750218960167	18.802238347554184
30	18.652552817947996	30.08778222083074	33.803724502534386	17.45594045868687
31	23.817872697945937	27.98719132561061	30.591806728517135	17.60312924792632
32	25.04103769285338	27.41995569276619	30.75544229953751	16.783564314842923
33	24.297880941152226	27.61513738016637	29.663455250882777	18.423526427798627
34	19.216182048040455	27.915459578566075	31.135144989279866	21.733213384113597
35	19.934926227266782	27.87111271395678	32.6165839043471	19.57737715442934
36	24.43944215686663	28.041723305684247	29.570917334115382	17.947917203333745
37	19.689968255729625	32.50355686425262	29.753971996877095	18.05250288314066
38	19.171795552631682	33.65265189494668	27.99202628314621	19.18352626927543
39	20.09186419317637	32.32799242259114	28.530450324774403	19.04969305945809
40	22.2721922569344	28.349496094384712	28.99004870625255	20.388262942428337
41	17.967098510278248	28.26623178469379	29.335946324045192	24.430723380982762
42	19.259855189058726	28.54459852018595	28.66856366093473	23.52698262982059
43	19.80652243697712	28.27035338783889	29.77917718534136	22.143946989842625
44	18.88803902841132	30.433085376631304	31.32624470433442	19.352630890622958
45	18.07865921079226	33.84712022795636	28.526566506426132	19.547654054825248
46	20.102841924630148	32.445577004624916	28.825065688050127	18.626515382694812
47	19.433160675150297	29.796575106309618	29.234253692599744	21.53601052594034
48	19.43744080149329	28.67280415647825	32.48433592650864	19.405419115519816
49	19.637219661632237	28.361187180228985	32.385219297028875	19.6163738611099
50	18.75947671492377	32.77637528781868	29.600957480115248	18.863190517142304
51	18.189665080113663	32.50359649505209	29.273646707275024	20.033091717559216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6350.0
1	5285.0
2	4220.0
3	20611.5
4	37003.0
5	26750.0
6	16497.0
7	16630.0
8	16763.0
9	17267.5
10	17772.0
11	18530.0
12	19288.0
13	19171.5
14	19055.0
15	18233.0
16	17411.0
17	17050.0
18	16689.0
19	16346.0
20	16003.0
21	17300.5
22	18598.0
23	20101.0
24	21604.0
25	24065.5
26	34116.0
27	41705.0
28	48450.5
29	55196.0
30	63642.0
31	72088.0
32	81368.0
33	90648.0
34	99022.0
35	107396.0
36	117261.0
37	127126.0
38	137756.0
39	148386.0
40	167181.0
41	185976.0
42	204102.5
43	222229.0
44	219709.5
45	217190.0
46	213620.0
47	210050.0
48	190939.0
49	171828.0
50	157866.5
51	143905.0
52	128739.5
53	113574.0
54	101073.0
55	88572.0
56	79559.5
57	70547.0
58	64351.0
59	58155.0
60	51191.0
61	44227.0
62	39740.5
63	35254.0
64	28667.5
65	22081.0
66	18391.0
67	14701.0
68	12534.5
69	10368.0
70	8717.5
71	7067.0
72	6111.0
73	5155.0
74	4117.5
75	2179.5
76	1279.0
77	1102.5
78	926.0
79	718.5
80	511.0
81	345.5
82	180.0
83	120.0
84	60.0
85	48.0
86	36.0
87	23.5
88	11.0
89	7.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2523290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.012436883886572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77728209719488	16.132777941836622
2	6.750394646350563	2.8368448289553405
3	2.7738224398710876	1.7485430683469847
4	1.7086865316729531	1.4361467160449992
5	1.2001302639682014	1.260883071203698
6	0.9047016281950102	1.1405991516718241
7	0.6715128742028189	0.9877085320132799
8	0.560089085136104	0.9415069240620926
9	0.45238324716624634	0.855510698556757
>10	6.052026159604233	30.352817876007656
>50	1.50306781931504	22.114374285991268
>100	0.6425958891804368	18.54448311334421
>500	0.0021400293862712896	0.2859098395148762
>1k	7.781925040986508E-4	0.21105983720550556
>5k	1.945481260246627E-4	0.23906215920546126
>10k+	1.945481260246627E-4	0.9117719560394336
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22304	0.8839253514261143	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5848	0.23176091531294463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017675336564564516	0.0
2	0.0	0.0	0.0	0.06356780235327687	0.0
3	3.963079947211775E-5	0.0	0.0	0.10763725136627181	0.0
4	3.963079947211775E-5	0.0	0.0	0.15899876748213643	0.0
5	3.963079947211775E-5	0.0	0.0	0.3225947077030385	0.0
6	3.963079947211775E-5	0.0	0.0	0.5099691276072112	0.0
7	3.963079947211775E-5	0.0	0.0	0.6459820313955194	0.0
8	7.92615989442355E-5	0.0	0.0	0.889552924951155	0.0
9	7.92615989442355E-5	0.0	0.0	1.0245750587526603	0.0
10	7.92615989442355E-5	0.0	0.0	1.2321215555881408	0.0
11	7.92615989442355E-5	0.0	0.0	1.368808182967475	0.0
12	7.92615989442355E-5	0.0	0.0	1.511835738262348	0.0
13	1.1889239841635325E-4	0.0	0.0	1.5771076649929259	0.0
14	1.1889239841635325E-4	0.0	0.0	1.6139246777025233	0.0
15	1.1889239841635325E-4	0.0	0.0	1.6502661208184553	0.0
16	1.1889239841635325E-4	0.0	0.0	1.7103067820187137	0.0
17	1.58523197888471E-4	0.0	0.0	1.785526039416793	0.0
18	1.58523197888471E-4	0.0	0.0	1.9021594822632357	0.0
19	1.58523197888471E-4	0.0	0.0	1.9741686449040736	0.0
20	1.9815399736058877E-4	0.0	0.0	2.041580634806146	0.0
21	1.9815399736058877E-4	0.0	0.0	2.1151353986263963	0.0
22	1.9815399736058877E-4	0.0	0.0	2.208188515786929	0.0
23	1.9815399736058877E-4	0.0	0.0	2.3075825608630005	0.0
24	1.9815399736058877E-4	0.0	0.0	2.387597144997206	0.0
25	1.9815399736058877E-4	0.0	0.0	2.45485061170139	0.0
26	1.9815399736058877E-4	0.0	0.0	2.521271831616659	0.0
27	1.9815399736058877E-4	0.0	0.0	2.598234844191512	0.0
28	1.9815399736058877E-4	0.0	0.0	2.6744845023758663	0.0
29	1.9815399736058877E-4	0.0	0.0	2.7630593391960496	0.0
30	1.9815399736058877E-4	0.0	0.0	2.894118393050343	0.0
31	1.9815399736058877E-4	0.0	0.0	2.993710592123775	0.0
32	1.9815399736058877E-4	0.0	0.0	3.0869222324821957	0.0
33	1.9815399736058877E-4	0.0	0.0	3.179182733653286	0.0
34	1.9815399736058877E-4	0.0	0.0	3.2842439830538703	0.0
35	1.9815399736058877E-4	0.0	0.0	3.4081694930031823	0.0
36	1.9815399736058877E-4	0.0	0.0	3.5152519131768445	0.0
37	1.9815399736058877E-4	0.0	0.0	3.6300227084480974	0.0
38	1.9815399736058877E-4	0.0	0.0	3.7545426803894917	0.0
39	1.9815399736058877E-4	0.0	0.0	3.914294433061598	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	7.034892E-4	45.0	31
CGTTTTT	15865	0.0	43.0145	1
CGACGGT	285	0.0	42.63158	28
GCGTAAG	80	0.0	42.1875	1
GTTAGCG	150	0.0	42.0	1
ACGGGAT	1555	0.0	40.948555	5
ACGGGAC	830	0.0	40.120483	5
CTAGCGG	230	0.0	40.1087	2
TACGGGA	1170	0.0	39.423077	4
TAAGGGA	4370	0.0	37.6373	4
AGGGCGA	2095	0.0	37.5895	6
GGGCGAT	3975	0.0	37.584908	7
AAGGGAC	3335	0.0	37.57871	5
AACGTCG	30	1.14030845E-4	37.500004	13
AAGGGAT	5415	0.0	37.47922	5
GTAGGGA	3965	0.0	37.45271	4
AGCGGGA	2050	0.0	37.42683	4
CGGGATT	1770	0.0	37.37288	6
TAGGGAC	2805	0.0	37.299465	5
TCACGAC	320	0.0	37.265625	25
>>END_MODULE