FastQCFastQC Report
Thu 26 May 2016
SRR1545216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545216_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1443228
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59840.4146261020434748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36360.2519352451587691No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT29670.20558082298846753No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27220.18860498826242283No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17150.1188308430823127No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16050.1112090397359253No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15950.11051614852261736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT253.891304E-545.034
ACGTTAG253.891304E-545.01
TAGACCC207.0338877E-445.01
CTCGCAC207.0338877E-445.043
CGTCGAA253.891304E-545.042
CACCGTT207.0338877E-445.036
GTGCGAT253.891304E-545.08
CTCGATG600.044.9999961
TATAGCG600.044.9999961
CGGTCTA600.044.99999631
TCACGAC700.041.78571325
GCGATAC650.041.5384649
CGTTAGG1950.041.538462
ACGGGTA1200.041.2499965
TATACCC556.184564E-1140.90909215
CTACGAA3200.040.7812511
TACGAAT3200.040.7812512
CGAATAT3200.040.7812514
GCTACGA3200.040.7812510
ACGGGAT2550.040.5882345