##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545216_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1443228
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31430099748619	31.0	31.0	33.0	30.0	34.0
2	31.572617077828312	31.0	31.0	34.0	30.0	34.0
3	31.65051814404931	31.0	31.0	34.0	30.0	34.0
4	35.412125457654646	37.0	35.0	37.0	33.0	37.0
5	35.27003217786795	37.0	35.0	37.0	33.0	37.0
6	35.26588453106508	37.0	35.0	37.0	32.0	37.0
7	35.87331177055878	37.0	35.0	37.0	35.0	37.0
8	35.96576909538895	37.0	35.0	37.0	35.0	37.0
9	37.6146180645054	39.0	37.0	39.0	35.0	39.0
10	36.887022701887716	39.0	37.0	39.0	32.0	39.0
11	36.52502653773347	39.0	35.0	39.0	32.0	39.0
12	35.56758322316363	37.0	35.0	39.0	31.0	39.0
13	35.092338840432696	37.0	34.0	39.0	30.0	39.0
14	35.87519989911504	38.0	34.0	40.0	29.0	41.0
15	36.32443175991597	38.0	35.0	40.0	31.0	41.0
16	36.27623563290069	38.0	35.0	40.0	31.0	41.0
17	36.361284564878176	37.0	35.0	40.0	31.0	41.0
18	36.44654967891421	37.0	35.0	40.0	31.0	41.0
19	36.36905326116178	37.0	35.0	40.0	31.0	41.0
20	36.21770988367742	37.0	35.0	40.0	31.0	41.0
21	36.077513047141544	37.0	34.0	40.0	31.0	41.0
22	35.968366051656425	36.0	34.0	40.0	30.0	41.0
23	35.89333494084095	36.0	34.0	40.0	30.0	41.0
24	35.72296684931279	36.0	34.0	40.0	30.0	41.0
25	35.69951941065445	36.0	34.0	40.0	30.0	41.0
26	35.56701020213023	36.0	34.0	40.0	30.0	41.0
27	35.50992497373942	36.0	34.0	40.0	30.0	41.0
28	35.570118512113126	36.0	34.0	40.0	30.0	41.0
29	35.587960460855804	36.0	34.0	40.0	30.0	41.0
30	35.52666661123537	36.0	34.0	40.0	30.0	41.0
31	35.40079252896978	36.0	34.0	40.0	30.0	41.0
32	35.25267871743065	36.0	34.0	40.0	29.0	41.0
33	35.07247434223837	36.0	34.0	40.0	29.0	41.0
34	34.90649155919924	35.0	34.0	40.0	27.0	41.0
35	34.73667362329445	35.0	34.0	40.0	27.0	41.0
36	34.519334436416145	35.0	33.0	40.0	25.0	41.0
37	34.325726080702424	35.0	33.0	40.0	25.0	41.0
38	34.37905237426103	35.0	33.0	40.0	25.0	41.0
39	34.3808157823989	35.0	33.0	40.0	25.0	41.0
40	34.20386730301796	35.0	33.0	40.0	24.0	41.0
41	34.248648169242834	35.0	33.0	40.0	24.0	41.0
42	34.195703658742765	35.0	33.0	40.0	25.0	41.0
43	34.06861632396267	35.0	33.0	40.0	24.0	41.0
44	34.03263586903802	35.0	33.0	40.0	24.0	41.0
45	33.955962606047	35.0	33.0	39.0	24.0	41.0
46	33.88610531392129	35.0	33.0	39.0	23.0	41.0
47	33.920605753214325	35.0	33.0	39.0	24.0	41.0
48	33.88600345891294	35.0	33.0	39.0	24.0	41.0
49	33.86482870343425	35.0	33.0	39.0	24.0	41.0
50	33.64122508709642	35.0	33.0	39.0	24.0	41.0
51	33.47212429359741	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	4.0
13	8.0
14	7.0
15	50.0
16	107.0
17	308.0
18	671.0
19	1418.0
20	2500.0
21	4191.0
22	6430.0
23	9137.0
24	13125.0
25	17596.0
26	22907.0
27	27392.0
28	30939.0
29	35637.0
30	43887.0
31	54625.0
32	68784.0
33	91869.0
34	157074.0
35	226158.0
36	101154.0
37	121861.0
38	165756.0
39	239449.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.29300706471881	24.04159287375245	28.860166238459893	13.805233823068845
2	31.224241769145276	26.552630630780445	29.60433140155263	12.618796198521649
3	28.90568919117423	26.93579947173974	31.319445021853788	12.839066315232243
4	27.11913848678102	27.207274249113794	31.290620747380178	14.382966516725007
5	23.910497856194585	32.13123636736538	29.514879145914573	14.443386630525461
6	21.649039514200112	41.67927728674887	26.831242187651572	9.840441011399447
7	86.09110965142028	5.523659463369613	6.620644832278753	1.7645860529313457
8	87.94182208216581	4.491459422904766	6.072845039037491	1.4938734558919313
9	83.71768008935526	5.775040395557736	7.391624885326505	3.115654629760509
10	53.824274473610544	22.325994229601974	12.907939701835053	10.941791594952425
11	47.67611215968648	20.70975618543986	19.209715997749488	12.40441565712417
12	42.99043532969149	21.854966782795234	22.578345209488727	12.576252678024538
13	21.34624605398454	42.8448588857755	21.949962168139752	13.85893289210021
14	14.837225996169698	43.994850432502695	26.567735659230557	14.600187912097049
15	13.805164533947512	24.318402913468972	48.12219552281414	13.754237029769378
16	15.208823553866749	19.62212484791038	46.244876069477584	18.924175528745284
17	15.601207847963039	20.085253334885408	28.93264265937191	35.380896157779645
18	19.576532606074714	24.45226949588007	36.38468765849886	19.58651023954635
19	31.850615425975658	22.90615204250472	25.84033846349988	19.402894068019744
20	32.7255291610196	22.49589115510508	25.810821297812957	18.967758386062357
21	21.50644250250134	30.66036689975527	28.0577982134493	19.775392384294097
22	21.761911492847975	25.399382495350697	23.735334957470336	29.103371054330985
23	19.13654668562417	32.34388468072959	25.42730601124701	23.092262622399232
24	17.847907607114053	23.960316734431427	41.51887297086808	16.67290268758644
25	16.985396624788322	26.352454359255777	37.92540056041042	18.736748455545484
26	15.719276510710712	37.41293821904786	26.543484466764777	20.32430080347665
27	16.729234743228375	38.810430507168654	27.478679737366512	16.98165501223646
28	14.952869539670793	30.78474087254405	38.05670344533227	16.20568614245289
29	15.339987860545943	26.030259945067584	37.16065652828243	21.46909566610404
30	17.828991676990746	35.07387606116289	29.734525660533194	17.362606601313168
31	30.040367842087324	27.766853192981287	24.61114945109158	17.581629513839808
32	32.1461335284515	25.67944912376977	26.345040423273385	15.829376924505345
33	30.62288148511531	27.31328660474991	23.13307391486307	18.930757995271712
34	19.56128899938194	27.375231079219635	26.813296305226896	26.250183616171523
35	21.5457294342959	24.61544537661409	31.601521034791453	22.23730415429856
36	31.528975324758115	27.437660577538686	23.932601085899112	17.100763011804094
37	20.218149869597873	37.123448270127795	26.58436504834995	16.07403681192438
38	19.581867868417188	37.825554936572736	22.70167984545754	19.89089734955253
39	21.562774558143275	35.14108650885376	25.07254571003334	18.22359322296962
40	25.443450376517085	26.33714146344167	26.020005155110624	22.199403004930616
41	17.028078723528093	25.582860088634646	27.15225868677714	30.236802501060122
42	19.798327083454588	25.747560330037945	24.498970363657023	29.955142222850444
43	20.292704964149806	26.14236974338081	26.267713763868215	27.29721152860116
44	18.090488820893164	30.613596742856984	30.490331396009502	20.80558304024035
45	16.693550845743015	40.56995845424285	22.74165966846541	19.994831031548724
46	19.80186082864246	37.21567209061909	24.758111677434197	18.22435540330426
47	21.493000412963163	28.177806971594233	26.182003120782028	24.14718949466058
48	21.788934250166985	25.959654330431505	32.93519804216658	19.31621337723492
49	21.06382359544022	26.849118780954917	33.54217074502435	18.544886878580517
50	19.24276690862428	36.86797927978115	25.983836233775953	17.90541757781861
51	18.55770536602671	35.76385713137494	24.880199109219056	20.798238393379286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4190.0
1	4158.5
2	4127.0
3	7475.5
4	10824.0
5	8156.0
6	5488.0
7	5594.5
8	5701.0
9	5963.5
10	6226.0
11	6537.0
12	6848.0
13	6805.5
14	6763.0
15	6606.0
16	6449.0
17	6133.0
18	5817.0
19	6192.5
20	6568.0
21	6450.5
22	6333.0
23	7094.5
24	7856.0
25	8319.5
26	10952.5
27	13122.0
28	18186.5
29	23251.0
30	25129.0
31	27007.0
32	32932.5
33	38858.0
34	41831.5
35	44805.0
36	46971.0
37	49137.0
38	57439.5
39	65742.0
40	89335.0
41	112928.0
42	134942.0
43	156956.0
44	157232.0
45	157508.0
46	144114.5
47	130721.0
48	120421.5
49	110122.0
50	95166.0
51	80210.0
52	72446.0
53	64682.0
54	58592.0
55	52502.0
56	47999.0
57	43496.0
58	42013.0
59	40530.0
60	37756.0
61	34982.0
62	32591.0
63	30200.0
64	25368.0
65	20536.0
66	18228.5
67	15921.0
68	13855.0
69	11789.0
70	10437.5
71	9086.0
72	8090.5
73	7095.0
74	5579.5
75	3383.0
76	2702.0
77	2213.0
78	1724.0
79	1372.5
80	1021.0
81	653.0
82	285.0
83	230.0
84	175.0
85	129.0
86	83.0
87	46.0
88	9.0
89	5.0
90	1.0
91	1.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1443228.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.520551989818774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.34166517948748	13.725892177461855
2	7.0155128387800065	2.458313148541724
3	2.468590041806423	1.2975318050705498
4	1.3233644960501219	0.9274430581810591
5	0.8357766162999476	0.7321633828879024
6	0.6152482022553544	0.6467692868554482
7	0.4827009255734256	0.5920030663429993
8	0.40674668217026066	0.5701141113320275
9	0.3590818648535092	0.5662181233590288
>10	5.39633572963728	23.764434773685352
>50	1.5213384443666778	18.45200896379128
>100	1.2226207013706776	33.923883499948126
>500	0.007753602578786006	0.8221627469147688
>1k	0.0028565904237632654	1.0932140534243688
>5k	4.08084346251895E-4	0.42784780220349394
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5984	0.4146261020434748	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3636	0.2519352451587691	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2967	0.20558082298846753	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2722	0.18860498826242283	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1715	0.1188308430823127	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1605	0.1112090397359253	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1595	0.11051614852261736	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014412137236805272	0.0
2	0.0	0.0	0.0	0.05848001840319063	0.0
3	0.0	0.0	0.0	0.08758144936212435	0.0
4	0.0	0.0	0.0	0.11966231253828225	0.0
5	0.0	0.0	0.0	0.21403409579082447	0.0
6	0.0	0.0	0.0	0.2926772485012763	0.0
7	0.0	0.0	0.0	0.3493557497498663	0.0
8	0.0	0.0	0.0	0.4858553187715316	0.0
9	0.0	0.0	0.0	0.5514721166717941	0.0
10	0.0	0.0	0.0	0.6944155739772233	0.0
11	0.0	0.0	0.0	0.8248177003217787	0.0
12	0.0	0.0	0.0	0.9489145166252317	0.0
13	6.928912133079458E-5	0.0	0.0	0.9953382279168641	0.0
14	6.928912133079458E-5	0.0	0.0	1.015570651345456	0.0
15	6.928912133079458E-5	0.0	0.0	1.046335021216329	0.0
16	6.928912133079458E-5	0.0	0.0	1.1129911559365533	0.0
17	6.928912133079458E-5	0.0	0.0	1.1896249241284123	0.0
18	6.928912133079458E-5	0.0	0.0	1.3130288492185573	0.0
19	6.928912133079458E-5	0.0	0.0	1.371162422015094	0.0
20	6.928912133079458E-5	0.0	0.0	1.4303353316315925	0.0
21	6.928912133079458E-5	0.0	0.0	1.506761232459459	0.0
22	6.928912133079458E-5	0.0	0.0	1.585404385169911	0.0
23	6.928912133079458E-5	0.0	0.0	1.6821319985477001	0.0
24	6.928912133079458E-5	0.0	0.0	1.7595279470741976	0.0
25	6.928912133079458E-5	0.0	0.0	1.8205716629666275	0.0
26	6.928912133079458E-5	0.0	0.0	1.8783587901565102	0.0
27	6.928912133079458E-5	0.0	0.0	1.940857577596887	0.0
28	6.928912133079458E-5	0.0	0.0	2.0060586407691647	0.0
29	6.928912133079458E-5	0.0	0.0	2.077079990133229	0.0
30	6.928912133079458E-5	0.0	0.0	2.1790735767321587	0.0
31	6.928912133079458E-5	0.0	0.0	2.263051991785082	0.0
32	6.928912133079458E-5	0.0	0.0	2.34924765872059	0.0
33	6.928912133079458E-5	0.0	0.0	2.426920763732411	0.0
34	6.928912133079458E-5	0.0	0.0	2.5084740595387562	0.0
35	6.928912133079458E-5	0.0	0.0	2.6263348549224377	0.0
36	6.928912133079458E-5	0.0	0.0	2.720152325204334	0.0
37	6.928912133079458E-5	0.0	0.0	2.8129997477875985	0.0
38	6.928912133079458E-5	0.0	0.0	2.9027291599109772	0.0
39	6.928912133079458E-5	0.0	0.0	2.994606534795611	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGT	25	3.891304E-5	45.0	34
ACGTTAG	25	3.891304E-5	45.0	1
TAGACCC	20	7.0338877E-4	45.0	1
CTCGCAC	20	7.0338877E-4	45.0	43
CGTCGAA	25	3.891304E-5	45.0	42
CACCGTT	20	7.0338877E-4	45.0	36
GTGCGAT	25	3.891304E-5	45.0	8
CTCGATG	60	0.0	44.999996	1
TATAGCG	60	0.0	44.999996	1
CGGTCTA	60	0.0	44.999996	31
TCACGAC	70	0.0	41.785713	25
GCGATAC	65	0.0	41.538464	9
CGTTAGG	195	0.0	41.53846	2
ACGGGTA	120	0.0	41.249996	5
TATACCC	55	6.184564E-11	40.909092	15
CTACGAA	320	0.0	40.78125	11
TACGAAT	320	0.0	40.78125	12
CGAATAT	320	0.0	40.78125	14
GCTACGA	320	0.0	40.78125	10
ACGGGAT	255	0.0	40.588234	5
>>END_MODULE